Myh8 (NM_001100485) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217711 representing NM_001100485 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTACGGGTTCGGACGCGGAGATGGCTATTTTTGGCGAAGCTGCTCCTTACCTCCGAAAATCGGAGA AGGAGCGGATTGAGGCCCAAAACAAGCCCTTTGATGCCAAAACATCTGTCTTTGTGGCGGAGCCCAAGGA GTCCTATGTGAAGAGCGTCATACAAAGCAAGGAAGGAGGGAAAGTGACCGTTAAGACGGAAAGTGGAGCA ACTCTCACCGTCAAGGAAGACCAAGTCTTCCCCATGAACCCTCCCAAATATGACAAGATCGAGGACATGG CCATGATGACCCACCTTCATGAGCCCGGGGTGCTGTATAACCTCAAAGAGCGTTACGCAGCCTGGATGAT CTACACCTACTCAGGTCTTTTCTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAACCCTGAA GTGGTGGCGGCTTACAGAGGCAAAAAGCGCCAGGAGGCCCCGCCCCACATCTTCTCCATCTCTGACAACG CCTACCAGTTCATGTTGACGGATCGTGAGAACCAGTCCATCCTGATCACCGGAGAATCTGGGGCCGGGAA GACTGTGAACACCAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTCACTGGGGAGAAGAAGAAAGAG GAGTCTGGCAAAATGCAGGGGACCCTGGAGGATCAAATCATCAGTGCCAATCCCTTGCTGGAGGCCTTTG GGAATGCCAAGACTGTGAGAAATGACAACTCCTCTCGCTTTGGCAAGTTCATCAGAATCCACTTCGGTAC CACAGGGAAACTGGCATCTGCTGATATCGAAACATATCTTCTAGAGAAGTCTAGAGTCACTTTCCAGCTA AAGGCTGAGAGAAGTTACCACATTTTTTATCAGATTACCTCCAATAAGAAGCCAGAGCTAATTGAAATGC TCCTGATCACCACCAACCCATACGACTACGCCTTTGTCAGTCAGGGGGAGATCACAGTGCCCAGCATTGA TGATCAAGAAGAGTTGATGGCCACAGACAGTGCCATTGATATCCTGGGCTTCTCACCTGAAGAGAAAGTA TCCATCTATAAGCTCACGGGAGCTGTGATGCATTATGGGAACATGAAGTTCAAGCAAAAGCAGCGTGAGG AGCAGGCTGAGCCGGATGGCACTGAAGTGGCTGACAAGGCTGCCTACCTCCAGTCTCTGAACTCTGCTGA CCTGCTCAAAGCCCTCTGCTACCCCAGGGTCAAGGTTGGCAATGAGTACGTCACCAAAGGCCAGACGGTG CAACAGGTGTATAATGCAGTGGGCGCCCTGGCTAAAGCTGTCTATGAGAAGATGTTCCTGTGGATGGTCA CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATCGGGGTCTTGGACATCGCTGG CTTTGAGATCTTTGACTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAACGAGAAACTGCAACAG TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGACCTTCA TCGACTTCGGGATGGACCTGGCGGCCTGCATCGAGCTCATCGAGAAGCCACTGGGTATCTTCTCCATCCT GGAAGAGGAGTGCATGTTCCCCAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTATGACCAGCACCTG GGAAAATCCAACAACTTCCAGAAGCCCAAGCCTACCAAAGGCAAGGCGGAGGCCCACTTCTCCCTCATTC ACTATGCGGGCACCGTGGACTACAACATCACTGGTTGGCTGGACAAGAACAAGGACCCTTTGAATGACAC CGTGGTGGGGCTGTACCAGAAGTCAGCAATGAAAACTCTGGCCAGCCTCTTTTCCACATACGCCAGTGCT GAAGCAGATGGTGGTGCAAAGAAAGGAGCTAAGAAGAAGGGCTCTTCTTTCCAAACCGTGTCAGCCCTTT TCAGGGAAAACTTAAATAAACTGATGACCAACCTAAGAAGTACACACCCTCACTTTGTACGGTGTATCAT TCCCAACGAAACGAAAACTCCCGGTGCCATGGAGCACGAACTGGTCCTGCACCAGCTGAGGTGTAACGGG GTGCTGGAAGGCATCCGCATCTGCAGGAAGGGGTTCCCCAGCAGGATTCTGTATGGGGACTTTAAGCAGA GATACAAGGTGTTAAATGCAAGTGCTATTCCAGAGGGACAGTTCATCGACAGCAAGAAGGCCTCTGAGAA GCTTCTGGGCTCTATTGATATTGATCACACTCAGTATAAATTTGGACACACCAAGGTTTTCTTCAAAGCT GGTCTCCTGGGTCTCCTGGAAGAAATGAGAGATGAAAAGCTGGCCCAGATTATAACAAGAACACAGGCGG TCTGTAGGGGATACCTAATGAGAGTCGAGTACCAGAAAATGCTGCTGAGAAGGGAGTCCATCTTCTGCAT CCAGTACAACATCCGCGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTCTTCAAGATCAAG CCCCTGCTGAAGAGCGCTGAGACCGAGAAGGAGATGGCCACCATGAAGGAGGAGTTCCAGAAAACCAAGG ACGAGCTCGCCAAGTCGGAGGCAAAGAGGAAGGAGCTGGAGGAGAAAATGGTCACGCTTCTCAAAGAGAA GAACGACCTGCAGCTCCAGGTTCAATCTGAAGCAGACAGCTTGGCTGATGCTGAGGAAAGGTGTGAACAA TTGATTAAAAACAAAATCCAGCTGGAGGCCAAAATCAAAGAGGTGACTGAGAGAGCGGAGGATGAGGAGG AGATCAATGCCGAGCTCACAGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCAGAGCTGAAGAAGGACAT CGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACGGAGAACAAGGTGAAAAAC CTCACAGAGGAGATGGCGGGCCTGGACGAAACCATCGCCAAGCTGACCAAGGAGAAGAAGGCCTTGCAGG AGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCTGACCAAAGCCAA AACCAAGCTCGAACAGCAAGTGGATGATCTTGAAGGATCGCTAGAGCAAGAAAAGAAGCTGAGGATGGAT CTGGAAAGAGCCAAGAGGAAACTGGAGGGGGACCTGAAACTGGCCCAAGAGTCCACAATGGACATAGAAA ATGACAAACAGCAGCTGGATGAGAAACTCAAAAAGAAGGAGTTTGAAATCAGCAATCTAATAAGCAAAAT TGAAGATGAACAAGCTGTGGAAATCCAGTTGCAGAAGAAGATCAAAGAGCTGCAGGCCCGCATCGAGGAG CTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCAGGGCCAAAGCAGAGAAGCAGCGCTCTGACCTCTCCC GGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCCGGCGGGGCCACATCTGCTCAGGTGGAGTTGAA TAAGAAGCGGGAAACGGAGTTCCAGAAGCTTCGCAGGGACCTGGAGGAGGCCACCCTGCAGCATGAAGCC ACATCAGCCGCTCTTCGGAAGAAGCACGCGGACAGTATGGCGGAGCTCGGGGAGCAGATCGACAACCTGC AGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGCTGAAGATGGAGATCGACGACCTTAGCAGTAA TGCAGAGGCCATCGCCAAAGCCAAGGGAAACCTTGAGAAGATGTGCCGCACTCTGGAGGACCAGGTGAGC GAGCTGAAGAGCAAGGAGGAGGAGCAGCAGCGGCTCATCAATGAGCTGACTGCACAGAGAGCACGCCTGC AGACAGAAGCAGGAGAGTATTCCCGACAGCTGGATGAGAAAGATGCTTTAGTCTCTCAGTTGTCAAGGAG CAAACAGGCATCCACCCAGCAGATTGAGGAGCTGAAGCGACAGCTCGAGGAAGAAACCAAGGCCAAGAAC GCGCTGGCCCACGCCCTGCAGTCCTCCCGCCACGACTGTGACCTGCTTCGGGAACAGTACGAGGAGGAGC AGGAAGGCAAAGCCGAGCTGCAGAGGGCGCTGTCCAAGGCCAACAGCGAGGTGGCCCAGTGGAGGACCAA ATATGAGACGGATGCCATCCAGCGCACGGAGGAGCTGGAGGAGGCCAAGAAGAAGCTGGCTCAGCGTCTG CAGGCGGCTGAGGAGCACGTAGAAGCTGTGAACGCCAAGTGTGCTTCCCTGGAGAAGACAAAGCAGCGGC TGCAGAATGAGGTGGAGGACCTCATGCTGGACGTAGAGAGGACCAACGCCGCGTGTGCTGCCCTGGACAA GAAGCAGAGAAACTTCGACAAGGTTCTATCGGAATGGAGACAGAAGTATGAGGAAACTCAGGCTGAGCTT GAGTCCTGCCAGAAGGAGTCCCGTACTCTCAGCACAGAGCTGTTCAAGGTGAAGAACGCCTATGAGGAGT CTCTGGATCAACTGGAGACGCTAAGGAGAGAGAACAAGAATCTACAGCAGGAGATTTCTGACCTGACCGA GCAGATTGCAGAGGGAGGGAAGCATATCCACGAGCTGGAGAAAATAAAGAAGCAAGTGGAACAAGAGAAG TGTGAGATCCAGGCTGCTTTAGAGGAAGCAGAGGCATCTCTGGAGCACGAGGAGGGAAAGATCCTGCGCA TCCAGCTGGAGCTGAACCAAGTCAAGTCTGAGATCGACAGGAAGATTGCTGAGAAGGATGAGGAGATCGA CCAGCTGAAGAGAAACCACATTCGAGTCGTGGAGACGATGCAGAGCACGCTGGACGCCGAGATCAGGAGC AGGAACGACGCTCTGAGAGTCAAGAAGAAGATGGAGGGCGACCTCAATGAGATGGAAATCCAGCTGAACC ACGCCAACCGCTTGGCTGCCGAGAGCTTGAGGAACTACAGGAACACCCAAGGCATCCTCAAGGACACCCA GTTGCACCTGGATGACGCTCTCCGGGGCCAGGAGGACCTGAAGGAGCAGTTGGCGATCGTGGAGCGCAGA GCCAACCTGCTGCAGGCTGAGATCGAGGAGCTGCGGGCCACTCTGGAGCAGACGGAGAGGAGCAGGAAGA TTGCAGAGCAGGAGCTGCTGGACGCCAGTGAGCGCGTGCAGCTCCTCCACACCCAGAACACGAGCCTCAT CAACACCAAAAAGAAGTTGGAAAATGATGTTTCACAGCTGCAGAGTGAAGTGGAGGAAGTAATTCAAGAG TCACGCAATGCAGAAGAGAAGGCCAAGAAAGCCATTACTGACGCCGCCATGATGGCGGAGGAGCTGAAGA AGGAGCAGGACACCAGCGCCCACCTGGAGCGGATGAAGAAGAACCTGGAGCAGACGGTGAAGGACCTGCA GCACCGTCTGGACGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATCCAGAAACTGGAGGCC AGGGTACGTGAACTTGAAGGAGAGGTTGAGAATGAACAGAAACGTAATGCTGAGGCCGTTAAAGGGTTAA GGAAGCACGAAAGAAGAGTAAAGGAACTTACCTACCAGACCGAGGAGGACCGCAAGAACGTGCTGAGGCT GCAGGACCTGGTGGATAAATTACAGGCGAAGGTGAAATCTTACAAGAGACAAGCAGAGGAGGCTGAGGAA CAATCCAACGTCAACCTGGCCAAGTTCCGCAAGCTGCAGCACGAGCTGGAGGAAGCCGAGGAGCGGGCAG ACATCGCCGAGTCCCAGGTCAACAAGCTGCGGGTGAAGAGCCGCGAGGTTCACACCAAAATCAGTGCAGA GTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100485
Insert Size	5814 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100485.1, NP_001093955.1
RefSeq Size	6026 bp
RefSeq ORF	5814 bp
Locus ID	252942
Gene Summary	heavy chain of myosin; involved in muscle contraction [RGD, Feb 2006]

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