Helq (NM_001014134) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Hel308
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217448 representing NM_001014134 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAAAGTTCTCCCCGCATCCGCCGGCGGGTGTCTCTCCGCAAAAGAAACCGCCGCAGCCTAGAGA GCCTCGGTGCTGCCCGGGCCCCAGCCGAGCTCCAGCCCGCCGAGGACACGGAGGACGAAGCTGCGGCTGG GAGCCGCCGGCGGAAAACCGGGAGCCCGGAGCCCGCCCAGGAAAATGACAGTGAAGAGGACATGTTTGGT GACTATAACAGCTTTTCAGAAAATTCCTTCTTAGCTCAAGTTGATGACCTGGAGCAGAAATATATACAAC TGCCTGACTGTGGGAGCCGAGGTACAGACTCCGGCACCAAAGACCTCTGTTCAGAAGGCCTCGGGAACAG CCTCAGTGTTCCTGCTGTAGATTTCACTGACTCTGAAACCAGAGAGTACATAAAGAAGCAGGGTGATCTA GATGTCCCTGTTGAGCCAGAGGTAGGAGGCGATCTTTCATTCGATATGCCTTCTTCTCAAATCTTATACT TTGAAAGTATGCAGAACTCCTCAAAAGATTTGGGTGATCAGTCCACCAAGGGAAGAGATGGGAAGTCATC GAATGAAGAATTGCATCATAGCCACAGAGAGCAACCCCAGCCAAAGAGCGACTTCTCTGACGTCGGCGGC TCCTCAGAGGCGAGCAGGAGGAGAAGTATCAAAGACTGCCTGAAAAGCGCCATGACTGGGAATGCCAGGG CCCAGACCCCAGCATTTCCCAGGAGTAAACAACTCAGGGAGACACTGCTGTCTGAGGAGATCAGCGTTGC TAGGAAAGCCATCGAGTCACCGTCAGATGATCTTGGTCCCTTTTATTCACTACCCAGCAAAGTGAGAGAC CTCTATGCTCAGTTGAAAGGAATCAAAAGACTATATGACTGGCAACATACTTGCTTAACGTTAAGTTCTG TGCAAGAAAGGAAAAATTTAATATACTCCTTGCCAACGAGTGGCGGAAAGACCCTTGTGGCCGAGATCTT AATGCTGCAGGAACTTCTCTGCCGACAGAAAGACGTTTTACTGATCCTTCCCTATGTGGCCATTGTGCAA GAAAAGATTTCCAGTTTGTCGAGTTTCGGTATAGAACTTGGTTTCTTTGTCGAAGAATATGCTGGCAGCA AAGGAAGATTTCCTCCAATTAAAAGGAGGGAAAAGAAGTCGCTGTACATCGCCACCATTGAGAAAGGGCA TAGCCTGGTGAACGCCTTGATTGAAACGGGAAGGATGGGCACTCTAGGCCTGGTTGTCGTCGATGAGTTG CACATGATTGGTGAGGGGAGCCGTGGTGCCACACTGGAGATGACCCTGGCCAAAGTCCTCTACACCAGCA AAACAACTCAGATTATCGGCATGAGTGCAACACTAAACAACGTAGAAGACCTACAGGCGTTCCTGAAAGC TGAGTATTACACCAGTCAGTTCAGACCAGTTGAATTAAAAGAATTCCTGAAAGTAAATGACACAATATAT GAGGTAGACAGCCGGGCTGTGGACGGCATGACCTTTTCACGGCTCCTGAATTATAAGTACTCCGATGCCC TGAAGAAAGTGGACCCTGACCGCTTGGTAGCATTGGTGACAGAAGTCATCCCTAACTACTCCTGCCTGGT TTTTTGTCCCAGTAAGAAGAACTGTGAAAATGTAGCAGAAATGTTGTGCAAGTTTTTAAGCAGGGACTAT CTAAACCACAGAGAGGAAGAAAAGGGGGAACTGATTAAAAGCCTGAGGAATGTCGGCCACGGTAAAGTGT GCCCTGTCCTGAAGCGCACCATCCCTTTCGGCGTCGCCTATCACCACAGCGGCTTAACTAGCGATGAGCG GAAGCTTCTGGAAGAGGCCTACTCCACTGGTGTGCTCTGCCTCTTCACCTGCACGTCCACCCTGGCAGCA GGGGTCAATCTGCCTGCACGGAGAGTCATCTTAAGAGCTCCCTACGTGGCTAACACGTTCTTGAAGAGGA ATCAGTATAAGCAGATGATTGGCAGGGCTGGCCGAGCTGGGATAGACACTGCCGGGGAGAGCATCCTTCT GCTGCAAGAGAAAGACAAGCAGCAGGTGCTGGGACTAATAAATGGACCGCTAGAAGACTGCCACAGCCAT CTTGTCGAGGAGTTCACCAAGGGAATCCAGAGTTTGTTCCTCTCCTTAATTGGCCTGAAGATTGCAGAGA GTCTCGGGGACATATATCAGTTCATGAATGGCACGCTTTTTGGCGTCCAGCAAAAGACTTTACTGAGAGA GAAAAGCCTCTGGGAAGTTACTGTCGGCGCACTTGAACACCTAACAGAGAAAGGGCTTCTACAGAAAGAC AGCCACGGCAGCCCCGAGGAGTCACGCTGTCACTTCCGTATTACCAAACTGGGTCAAGCTTCTTTTAAGG GGGCCATTGACTTGACCTATTGTGACACTCTGTACAGAGACTTGAAGAAAGGTCTGGAAGGACTCGTACT GGAAAGCCTTCTTCATCTGGTCTACCTAACCACACCCTATGACCTCGCCGCTCAGTCTGAGCCTGACTGG ATGGTGTACTTCAGGCAGTTTAGTCAACTCAGCCCAGCAGAGCAAAGTGTGGCTACTCTTCTTGGAATCT CCGAAAGCTTTATTGGGAGAAAGGCGTCAGGACAAGCCATCAGAAAGAAGGTGGACAAGAACGCTGTCAA CAGGCTCTATCTGTCTTTTGTGCTTTATTCCTTGCTGAAAGAGACCAACGTTTGGAGCGTGTCTGAGAGA TTTAACATGCCCCGGGGATACATACAGAACCTGCTTATGGGAGCTGCCTCGTTCTCATCCTGTGTGTTAC ATTTCTGTGAGGAGCTGGAGGAGTTTTGGGTTTACAAGGCCCTGTTGACGGAACTCACCAAGAAGCTGAC GTACTGCGTGAAGGCGGAGCTAATCCCTCTCATGGAGGTGACAGGGGTCTTAGAGGGTCGAGCAAAACAG TTATACAGCGCTGGTTACAGAAGTGTTGTGCACTTAGCCAATGCCAACCCAGAAGTGCTGGTGAAGACAA TTGATCATCTGTCCAGACGCCAAGCCAAGCAGATTGTTTCCTCAGCCAAGATGCTGCTGCATGAAAAGGC AGAGGCGTTGCAGGAAGAAGCAGAAGAGTTGCTGAGATTGCCTGCTGACCTCCCTGGGCTGGGGGGTCCC GGCTCTGAAAGGGCAGGAAGCCATGCTGGGGACCTGGCTCTGCGATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001014134
Insert Size	3198 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001014134.2, NP_001014156.2
RefSeq Size	3728 bp
RefSeq ORF	3198 bp
Locus ID	360912

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