Capn9 (NM_001271140) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Ncl-4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217108 representing NM_001271140 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTTACCTGCATCGGTCCCTGAGACCCCAGCCACAGCCAGTCCCCAGGGATGCCAGGACTGTCCACT CCTCGGGCCACAGCTTTGAGCAGCTGAGGCAAAGCTGTCTGCAGTCGGGCACCTTGTTTGAGGATGCTGA CTTCCCTGCCAGCAATAGTTCACTGTTCTACAGCGAGAGGCCCCAGGTCCCCTTTGTGTGGAAGCGGCCA GGGGAAATTGTGGAAAACCCAGAATTCATTCTGGGAGGGGCCACCAGGACCGACATCTGCCAAGGGGAAC TGGGAGACTGCTGGCTCCTGGCCGCCATTGCCTCCCTAACCCTCAATCAGAAAGCACTGGCCAGAGTGGT ACCCCAGGACCAAGGATTTGGTTCTGGCTATGCTGGGATATTTCACTTCCAGTTCTGGCAGCACAGTGAG TGGCTGGACGTGGTGATTGATGACCGCCTGCCTACCTTTCGGGATCGCCTGGTCTTCCTCCACTCTGCCG ACCACAATGAGTTCTGGAGCGCACTGCTGGAGAAAGCCTATGCCAAGCTGAACGGGAGTTACGAGGCACT GAAGGGGGGCAGCGCCATCGAAGCCATGGAGGACTTCACCGGGGGTGTGGCTGAGAACTTCCAAATCCGA GAGGCACCAGAGGATTTCTACGAGATTCTGGAGAAGGCCCTGCGGCGTGGTTCCTTGCTGGGCTGCTCCA TAGATACCCTGAATGCCTCAGAATCTGAAGCCCGCACACCTTTGGGCCTCATTAAGGGCCATGCCTACAC TGTGACTGGACTCGACCAGGTAAACTTCCACGGCCAGAGAATCAAGCTCATCAGAGTCCGTAACCCTTGG GGCCAGGTGGAATGGAACGGGCCATGGAGCGACAGCTCTCCTGAGTGGCGCTCCATGAGCCTGGAGGAGC AGAAGCGCCTAGGTCACACCGCCCTTGACGATGGAGAATTCTGGATGGCATTTGAGGACTTCAAGACCCA TTTTGACAAAGTGGAGATCTGCAACCTCACGCCTGATGCCCTGGAGGACAACACCCTCCACAAGTGGGAG GTGACCATCCACCAAGGAAGCTGGGTTCGTGGCTCCACCGCCGGCGGCTGCCGCAACTTCCTGGACACCT TCTGGACCAACCCACAGATAAAGCTGTCTCTGACAGAGAGGGATGAGGGCCAGGAGGGCTGCACTTTCCT AGCAGCCTTGATGCAGAAAGACCGCAGGAGGCTCAAGAGGTTTGGTGCCAACATGCTGACCATTGGCTAT GCCATTTACCAGTGCCCAGACAAGGACGGACACCTGAATAGGGACTTTTTCAGGTACCATGCCTCTCTGG CCCGAAGTAAGACATTCATCAACTTGAGAGAAGTCTCAGGCCGCTTCCAGCTGCCCCCGGGAGACTATAT CCTCATCCCCAGCACCTTTGAGCCACACCAGGAGGCTGACTTCTGCCTGAGAATCTTCTCTGAGAAGAAA GCTGTTACTCAGGACCTGGATGAGAACATAGACATTGACCTTCCTGAGCTTCCAAAGCCGACACCACAGG AGGAGGAAACCGAAGAGGAGCGGCAGTTCCGGGCCCTGTTCCGTCGAATTGCTGGTGAGGACATGGAGGT GTCAGCAGAAGAGCTTGAATATGTTCTAAATGCTGTTCTGCAAAAGAAAACAGCCCTCAAGTTCAAGAGG CTGAGCCTGCTTTCCTGCAGAAACATTATCTCCCTGATGGACACCAGCGGCAACGGGAAGATGGAGTTCG AAGAGTTCCGGGTGTTCTGGGACAAGCTGAGGTACTGGATGGACCTGTTTCTCCAGTTCGATGTGGACAA GTCCGGCACCATGTCTTCCTATGAGTTGCGGACAGCACTGAAAGCTGCAGGCTTTCAGCTGGGTAGCCAT CTCCTGCAGCTGATCGTCCTCAGGTATGCAGACGAGAACCTCCAGCTGGATTTCGATGACTACCTCAACT GCCTGGTGCGGCTGGAGAATGCGAGCCGGGTGTTCCAGTGTCTCAGTGTGAAGAACAAGGACTTCATCCA TCTCAACATAAACGAGTTCATCAACTTGACCATGAACATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271140
Insert Size	2073 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271140.1, NP_001258069.1
RefSeq Size	2434 bp
RefSeq ORF	2073 bp
Locus ID	116694
UniProt ID	O35920
Gene Summary	member of the calpain large subunit family; predicted to have calpain cysteine protease activity [RGD, Feb 2006]

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