Slco1b2 (NM_001270586) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	OATP-4; Oatp4; rlst-1; Slc21a10; Slco1b3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217056 representing NM_001270586 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCACACTCAGCAGTCAAGGAAAGCTGCAGAGGCCCAACCTTCACGATCAAAGCAAACAAGGTTCT GCGATGGATTCAAGCTATTTTTGGCAGCCCTCTCCTTCAGCTACATATGCAAAGCACTAGGTGGAGTTGT TATGAAGAGTTCCATCACCCAAATCGAAAGAAGATTCGACATACCATCTTCCATTTCTGGTTTGATTGAT GGAGGCTTTGAAATTGGGAATTTATTAGTTATTGTATTTGTGAGTTACTTTGGATCCAAACTACACAGGC CAAAGCTGATTGGAATTGGCTGCTTCATCATGGGCATTGGGAGCATTTTGACAGCGTTGCCACATTTCTT CATGGGATATTACAAGTATGCAAAAGAAAACGACATTGGCTCTCTAGGCAACTCTACATTGACCTGTTTC ATCAATCAAATGACATCACCCACTGGACCTTCACCTGAGATAGTGGAGAAAGGTTGTGAAAAGGGGTTAA AGTCACACATGTGGATTTATGTCTTGATGGGGAACATGCTTCGTGGGATAGGGGAAACACCAATAGTGCC CCTGGGAATTTCCTACCTTGATGACTTTGCAAAAGAAGGGCACACTTCCATGCACCTAGGTACTCTGCAT ACTATAGCAATGATTGGACCAATCCTTGGCTTTATCATGTCATCTGTGTTTGCTAAGATATATGTAGACG TTGGATATGTAGACCTGAACAGTGTCCGAATAACTCCTAATGATGCTCGTTGGGTTGGTGCCTGGTGGCT CAGCTTCATTGTGAATGGACTATTATGCATTACTTCTTCCATACCCTTCTTTTTTCTGCCCAAAATTCCA AAGAGGTCACAGGAGGAAAGGAAAAATTCAGTATCTCTTCATGCACCCAAAACAGATGAGGAGAAGAAAC ACATGACTAATTTAACAAAGCAAGAGGAACAAGATCCTTCCAACATGACTGGTTTTCTAAGGTCTCTGAG AAGCATCCTTACCAATGAAATATATGTTATATTCTTGATACTGACGTTACTACAAGTCAGCGGCTTCATT GGCTCCTTTACTTACCTGTTCAAGTTCATAGAGCAGCAGTTCGGCCGGACAGCATCTCAGGCCAACTTCT TGTTAGGAATTATAACCATACCTACTATGGCAACTGCAATGTTTTTAGGAGGATATATTGTTAAAAAATT CAAATTGACATCGGTTGGAATCGCCAAAATGAACCCAGTGGACTCTCACATAGATGTACCACTTTCTTAT TGTAACTCGGACTGCAGTTGTGATAAAAATCAATGGGAACCCATCTGTGGGGAAAATGGAGTCACTTACA TTTCACCTTGTCTGGCAGGATGCAAATCTTTTCGTGGTGATAAGAAGCCGAATAACACAGAGTTCTATGA CTGCAGTTGTATCAGCAACTCTGGAAACAACTCAGCACATTTGGGTGAATGCCCAAGATACAAATGCAAA ACCAACTATTACTTTTATATAATTCTTCAAGTCACTGTGTCCTTTTTCACTGCAATGGGAAGCCCATCTT TAATCTTGATTCTGATGAAGAGCGTCCAACCTGAATTGAAATCACTTGCAATGGGTTTCCATTCACTGAT TATTCGAGCACTAGGAGGGATTCTAGCTCCAATCTATTATGGGGCATTCATTGACAGAACGTGTATTAAG TGGTCTGTCACCAGCTGTGGAAAACGTGGTGCATGTAGGCTATATAACTCCAGATTATTTGGATTCTCCT ACTTGGGTTTGAACTTAGCTTTAAAAACTCCACCACTTTTTTTATATGTTGTATTAATTTATTTCACAAA GAGAAAATATAAAAGAAATGATAACAAGACATTGGAAAATGGAAGACAGTTCACAGATGAAGGAAACCCA GATTCTGTAAATAAAAATGGATACTATTGTGTACCTTATGATGAACAAAGCAATGAAACACCTCTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270586
Insert Size	1959 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270586.1, NP_001257515.1
RefSeq Size	3113 bp
RefSeq ORF	1959 bp
Locus ID	58978
UniProt ID	Q9QZX8
Gene Summary	mediated uptake of a variety of organic anions including taurocholate, bromosulfophthalein and steroid conjugates [RGD, Feb 2006] Transcript Variant: This variant (2, also known as rlst-1b) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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