Ttc17 (NM_001107752) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214366 representing NM_001107752 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCAATAGGGGTCCGCGGCCGGTTCGAGCTGCTATCTTGCTCCGGCCCAGGTTGGCTTATCA GCCTTTCCGCTTTGCTGAGCGTGGTGGCTCGAGGGGCCTTGGCCACTACGCACTGGGTCGTCACGGAGGA CGGGAAGATCCAACAGCAGGTGGACTCACCAATGAACTTGAAGCATCCCCATGACTTAGTCATCTTAATG AGACAAGAAACAACCGTTAACTACCTCAAAGAACTAGAGAAACAATTAGTTGCTCAAAAAATTCACATAG AGGAGAATGAGGACAGAGACACAGGACTGGAACAGAGGCACAATAAAGAAGACCCCGACTGCATCAAGGC CAAAGTGCCTTTGGGAGACCTGGACCTTTATGACGGCACATATATAACTCTAGAGAGCAAGGATGTCAGG CCTGAGGATTTCATAGACACAGAATCTCCTGTCCCTCCAGACCCAGAGCAACCTGATTGTACTAAAATCC TAGAGCTTCCATATAGTATACATGCTTTTCAGCATTTACGGGGTGTACAAGAGAGAGTTAATCTCTCTGC CCCTCTCTTACCTAAAGAAGATCCAATCTTCACATATTTGTCGAAACGCTTAGGAAGGAGTATCGACGAC ATAGGTCACCTCATTCACGAAGGCCTACAGAAGAACTCTTCCTCCTGGGTACTGTATAACCTAGCTTCAT TTTACTGGAGAATAAAGAATGAGCCATATCAGGTAGTGGAGTGCACCATGCGAGCTCTTCACTTCTCTTC CAGGCACAATAAAGACATTGCCCTGGTCAATTTGGCCAATGTTCTACACAGAGCGCATTTCTCCGCGGAT GCTGCTGTCGTGGTCCATGCAGCTCTGGATGACAGTGCCTTCTTCACCAGCTATTACACACTGGGGAATA TATATGCAATGCTCGGGGAGTATAACCATTCGGTGCTCTGTTACGACCATGCTTTGCAAGCCAAACCTGG GTTTGAGCAAGCTATAAAGAGGAAGCATGCTGTCCTGTGTCAACAAAAACTGGAGCAGAAGCTAGAGGCT CAGCATAGGTCCCTTCAGAGAACACTGAATGAGTTGAAGGAGTACCAGAAGCAGCATGACCACTACCTGC GGCAGCAGGAGATCCTAGAGAAGCACAAACTGATTCAGGAGGAGCAGATCTTGCGCAACATCATCCACGA GACACAGATGGCCAAAGAGGCACAACTGGGAAATCATCAGATATGCAGGCTGGTCAACCAGCAGCATAGT TTGCACTGCCAGTGGGACCAACCTGTACGCTACCATCGAGGAGACATCTTTGAAAATGTGGACTATGTTC AGTTTGGTGATGATTCATCGACCTCCAGTATGATGTCCGTGAACTTCGATGTCCCTACAAATCAGAGTGA CGTCAGCGAATCTGTCAGGTCTTCTCCTGTAGCGCATTCTGTTCTCTGGGTCTGGGGCCGTGACTCTGAT GCATATAGGGACAAACAACATATTCTTTGGCCTAAAAGAGCAGATTGTACAGACAGCTATCCCAGAGTTC CCCTCGGTGGAGAACTGCCAACATACTTCCTGCCTCCGGAGAACAAAGGACTCAGGATCCACGAACTGAC CAGTGATGATTATTCTTCAGAAGAGGAGGCCCAAACCCCTGACTGTTCCATAACTGACTTCAGAAAAAGC CACACTCTGTCCTACTTAGTCAAAGAATTAGAGGTCCGCATGGATCTGAAAGCCAAAATCCCCGATGACC ATGCACGCAAAATTTTACTTTCCCGTATTAAAAACTATACTATCCCAGAAGAAGAAATTGGGTCTTTCTT GTTTCATGCTATTAACAAGCCAAATGCTCCTGTCTGGCTCATTCTGAATGAGGCGGGCCTGTACTGGAGA GCAGTAGGAAACAGCACTTTTGCTATCGCCTGCCTTCAGAGGGCTTTGAATTTAGCTCCAGTTCAATACC AAGACATTCCTCTTGTCAACCTGGCCAACCTTTTGATTCATTATGGCCTTCATCTCGATGCTACAAAGTT GCTCCTGCAAGCACTGGCTGTCAACAGCTCCGAGCCCCTGACCTTTTTGAGCCTGGGAAATGCTTACCTT GCTCTGAAGAATGTCAGCGGGGCACTTGAAGCCTTCAGACAGGCCTTGAAGTTAACCACCAGATGCCCAG AGTGTGAGAGCAGCCTGAAGCTGATCCGCTGCATGCAGTTTTACCCTTTTCTGTACAACATCACCTCTTC TGTTTGTAGTGGTCATTGTCATGAGAAAACCCTGGACAACAGCCATGACAAACAGAAATACTTTGCCAAG CCACAGTCATTGGATGCTGCTGAAGAAGAGCCCTCTAGGCACGGAGCAGACGAGGACCCTGTGCTGTCTG TTGAGAACGCAGGGAGGGACTCAGATGCCCTTAGACTTGAAAGCACAGTGGTTGAAGAGAGCAACGGTTC TGACGAGGTGGAGAAGTCAGAGGAAACAAAGATGTCAGAAGAGATACTGGCCTTGGTGGATGAGTTTCAG CAGGCCTGGCCTCTGGAAGGTTTGGGGGGAACATTAGAAATGAAGGGGCGGCGTCTGGACCTGCAGGGAA TACGGGTCCTGAAGAAAGGGCCCCAGGATGGAGTAGCCAAGAGCTCTTGCTATGGAGACTGCAGAAGTGA AGATGATGAAGCAACAGAATGGATCACATTCCAGGTCAAGCGTGTAAAGAAACCCAAAGGGGATCATAAA AAACCTCCTGGGAAAAAAGTAGAGGCAAGTCAAGCAGAAAATGGACAGCGTTACCAAGCAAACTTAGAGA TCACAGGCCCAAAGGTGGCATCTCCTGGGCCCCAGGAAAAAAAGCGTGACTACCAGAGTATGGGGTGGCC CAGTCCAGATGAGTGCCTCAAGCTCCGCTGGGTAGAGCTAACTGCCATTGTGAGCACCTGGCTAGCAGTG TCTTCAAAAAACATTGACATCACAGAACACATAGACTTTGCCACACCCATACAACAGCCAGCAATGGAAC CTCTGTGCAATGGCAATCTCCCCACAAGCATGCATACCCTGGACCACTTACATGGAGTGTCCAACAGAGC CAGCCTGCACTACACCGGTGAGAGCCAGCTAACAGAGGTACTGCAGAATCTTGGCAAGGACCAGTACCCT CAGCAGTCGCTTGAACAAATCGGCACCCGAATTGCCAAGGTTTTGGAAAAAAATCAGACATCATGGGTCC TTTCCAGCATGGCAGCCCTCTACTGGAGAGTGAAAGGTCAGGGGAAAAAAGCCATCGACTGCCTGCGCCA GGCCCTCCACTATGCTCCCCACCAGATGAAGGATGTGCCCCTCATCAGCCTGGCCAACATCCTACACAAT GCCAAGCTCTGGAATGATGCTGTCGTCGTGGCCACCATGGCCGTGGAGATCGCGCCACACTTCGCTGTGA ACCACTTCACTCTGGGCAATGTCTATGTGGCAATGGAAGAATTTGAGAAGGCCCTGGTGTGGTACGAGTC AACGCTGAAGCTGCAACCAGAGTTTGTACCTGCCAAGAATCGAATCCAGACAATCCAGTGCCACCTCATG CTGAAGAAGGGCCGGCGCTCTCCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107752
Insert Size	3597 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107752.2, NP_001101222.2
RefSeq Size	4540 bp
RefSeq ORF	3597 bp
Locus ID	311224
UniProt ID	B5DEL3
Gene Summary	Plays a role in primary ciliogenesis by modulating actin polymerization.[UniProtKB/Swiss-Prot Function]

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