United States
Germany
Japan
United Kingdom
China
Brsk1 (NM_001127337) Rat Untagged Clone
CAT＃: RN210211
Brsk1 (untagged ORF) - Rat BR serine/threonine kinase 1 (Brsk1), (10 ug)
CNY 9,980.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | RGD1563268; Sad-b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN210211 representing NM_001127337
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTCCGGGTCCAAGGAAGGTGGCGGGGGCTCCCCCGCCTACCACCTCCCACACCCACACCCACACC CACCCCAGCACGCCCAATATGTGGGCCCCTATCGGCTGGAGAAGACGCTGGGCAAAGGACAGACAGGTCT AGTTAAACTTGGGGTCCACTGCATCACGGGTCAGAAGGTCGCTGTCAAGATCGTGAACAGGGAGAAGCTG TCGGAATCTGTGCTGATGAAGGTGGAGAGGGAAATCGCCATTCTAAAGCTCATTGAACACCCGCATGTGC TCAAGCTCCACGACGTCTACGAGAACAAGAAATATTTATACTTGGTGCTTGAGCATGTTTCTGGTGGTGA GCTGTTCGACTACCTGGTAAAAAAAGGGAGACTGACACCCAAGGAGGCCCGAAAGTTCTTCCGCCAGATT GTGTCAGCGCTGGACTTCTGCCATAGCTACTCCATCTGTCACAGGGACCTGAAGCCAGAGAACCTGCTGC TGGATGAGAAAAACAACATCCGCATCGCAGACTTTGGCATGGCGTCCCTGCAGGTGGGGGACAGCCTCCT GGAGACCAGCTGTGGGTCCCCCCATTACGCATGTCCAGAGGTGATCAAGGGGGAGAAGTATGATGGCCGC CGGGCAGACATGTGGAGCTGTGGAGTCATCCTGTTTGCCCTGCTTGTGGGGGCGCTGCCCTTCGATGATG ACAACCTACGACAGCTACTAGAGAAGGTGAAACGTGGTGTCTTCCACATGCCGCACTTCATCCCTCCAGA CTGCCAGAGCCTCCTGAGAGGGATGATTGAAGTGGAGCCCGAGAAAAGGCTCAGTCTGGAGCAAATTCAG AAACATCCTTGGTATCTGGGCGGGAAACACGAACCAGACCCTTGCCTGGAGCCAGCCCCAGGCCGCAGAG TAGCTATGCGGAGCCTGCCTTCCAATGGCGAGCTGGACCCTGACGTTCTGGAAAGCATGGCGTCTCTGGG CTGCTTCAGAGACCGCGAGCGGCTACACAGAGAACTGCGAAGTGAGGAGGAAAACCAAGAAAAGATGATC TATTATTTGCTTTTGGATCGGAAAGAGCGGTACCCTAGCTGTGAAGACCAGGACCTGCCTCCTCGGAATG ATGTTGACCCACCTCGGAAGCGTGTGGATTCCCCCATGCTGAGCAGACACGGGAAACGGAGGCCAGAGCG GAAGTCCATGGAAGTCCTCAGCATCACAGATGCTGGGAGTGGTGGCTCTCCAGTGCCTACCAGAAGGGCC TTGGAGATGGCCCAGCACAGTCAGAGATCCCGCAGCGTCAGTGGAGCATCCACTGGTCTGTCCTCCAGCC CTCTAAGCAGCCCAAGGAGTCCGGTCTTTTCCTTCTCACCGGAGCCAGGCGTTGGAGATGAGGCTAGAGG TGGAGGCTCCCCAACTTCCAAAACACAGACGCTGCCTTCTCGGGGCCCCAGAGGTGGGGGCGCTGGAGAG CAGCCACCACCTCCCAGTGCCCGTTCCACGCCCTTGCCTGGACCACCAGGCTCCCCGCGCTCCTCCGGCG GAACCCCCTTGCACTCACCTCTGCACACGCCTCGAGCCAGCCCTACCGGGACTCCAGGAACTACACCACC TCCCAGCCCAGGCGGTGGCGTCGGGGGAGCCGCCTGGAGAAGTCGTCTCAACTCCATCCGCAACAGCTTC CTGGGCTCCCCTCGCTTCCACCGGCGCAAGATGCAGGTCCCTACCGCTGAGGAGATGTCCAGTTTGACAC CAGAATCCTCTCCAGAGCTGGCAAAACGCTCCTGGTTCGGGAATTTCATCTCCTTGGACAAAGAAGAACA AATATTCCTCGTGCTAAAGGACAAACCTCTCAGCAGCATCAAAGCGGACATTGTCCATGCCTTTCTGTCG ATCCCTAGCCTGAGTCACAGTGTGCTGTCACAGACCAGCTTCAGGGCCGAATACAAGGCCAGCGGTGGCC CCTCTGTCTTCCAGAAGCCTGTCCGCTTCCAGGTGGACATCAGCTCCTCTGAGGGTCCAGAACCCTCACC CCGAAGAGATGGTAGCAGTGGAGGTGGCATCTACTCAGTCACCTTCACTCTCATCTCCGGTCCTAGCCGT CGGTTCAAGCGTGTGGTAGAGACCATCCAGGCACAGTTGCTGAGTACTCATGACCAGCCCTCCGTGCAGG CCTTGGCAGATGAGAAGAATGGAGCCCAGACCCGGCCTGCTGGTACCCCACCCCGAAGCCTGCAGCCTCC ACCAGGCCGCCCTGACCCTGATCTGAGTAGCTCTCCCCGCCGAGGACCCTCTAAGGACAAGAAGCTCCTG GCCACCAATGGAACCCCTCTACCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001127337 |
| Insert Size | 2337 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001127337.1, NP_001120809.1 |
| RefSeq Size | 2442 bp |
| RefSeq ORF | 2337 bp |
| Locus ID | 499073 |
| UniProt ID | B2DD29 |
| Gene Summary | Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-523' and 'Ser-573'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C (By similarity). In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR210211 | Brsk1 (Myc-DDK-tagged ORF) - Rat BR serine/threonine kinase 1 (Brsk1), (10 ug) |
CNY 9,980.00 |
|
| RR210211L3 | Lenti ORF clone of Brsk1 (Myc-DDK-tagged ORF) - Rat BR serine/threonine kinase 1 (Brsk1), (10 ug) |
CNY 11,880.00 |
|
| RR210211L4 | Lenti ORF clone of Brsk1 (mGFP-tagged ORF) - Rat BR serine/threonine kinase 1 (Brsk1), (10 ug) |
CNY 13,020.00 |
