Emc8 (NM_001012165) Rat Untagged Clone

CAT#: RN209675

Cox4nb (untagged ORF) - Rat COX4 neighbor (Cox4nb), (10 ug)



  "NM_001012165" in other vectors (3)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Cox4nb; DD6A4-2(5); Noc4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN209675 representing NM_001012165
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTGGCGTGAAGCTGACCACCCAGGCCTACTGCAAGATGGTGCTACACGGCGCCAAGTACCCGCACT
GCGCCGTCAACGGGCTTCTGGTGGCAGAGAGGCAGAGGCCGCGCAAGGAACATCCTCCCGGCGCGGGCAA
CCACACTCTCTTCGTGGACTGCATCCCGCTCTTCCACGGCACGCTGGCTCTGACGCCCATGCTGGAGGTG
GCGCTCACCCTAATTGACTCATGGTGCAAAGACAACAGCTATGTGATCGCCGGCTATTACCAAGCTAATG
AACGTGTGAAGGATGCCAGCCCAAACCAGGTGGCAGAGAAGGTGGCCTCCAGGATCGCAGAGGGCTTCAG
TGATGCTGCACTCATCATGGTGGACAACGCCAAGTTCACGATGGACTGTGCAGCGCCCACGATCCACGTG
TATGAGCAACACGAGAACAGGTGGCGGTGCCGAGACCCACATCACGACTACTGTGAAGATTGGCCGGAGG
CTCAGAGGATCTCCGCTTCGCTCCTGGACAGCCGCTCCTATGAGACACTTGTAGATTTTGATAATCACCT
GGATGACATTCGGAGCGACTGGACAAACCCAGAGATCAACAAAGCAGTTCTACACCTGTGCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001012165
Insert Size 624 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001012165.1, NP_001012165.1
RefSeq Size 1029 bp
RefSeq ORF 624 bp
Locus ID 361425
UniProt ID Q5FVL2
Gene Summary Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors. By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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