Sephs2 (NM_001079889) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209287 representing NM_001079889 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAAGCGGCGGCAGGCGCCAGCGGAGAAGCCATGGCGGCACTAGTGGCCGCGGAAGGTTCCTTGG GCCCGGCGGGCTGGTCTGCTGGCCGGAGTTTCTCCAACTACCGGCCGTTCGAGCCCCAGACACTGGGCTT CAGCCCGAGCTGGCGGCTGACGAGCTTCTCCGGCATGAAAGGCTGAGGCTGCAAGGTCCCCCAGGAGACC CTGCTCAAACTCCTGGAGGGACTGACGCGGCCCGCGCTGCAGCCCCCGCTTACCTCGGGTCTGGTCGGGG GCCAGGAAGAGACGGTGCAGGAAGGGGGCCTGACCACCAGGCCCGGCCCGGGCTCAGCCTTCCCCTCGCT GAGCATTGGGATGGACTCCTGCGTCATCCCCCTGAGGCACGGAGGCCTGTCGCTGGTGCAGACCACCGAC TTCTTTTACCCCTTGGTGGAAGATCCCTATATGATGGGGCGCATAGCTTGTGCCAATGTGCTCAGTGACC TCTATGCCATGGGCATTACCGAGTGTGACAACATGTTGATGTTACTCAGTGTGAGCCAGAGCATGAGTGA AAAGGAACGAGAGAAGGTAACGCCGCTCATGATCAAAGGCTTTCGTGACGCTGCGGAAGAGGGAGGAACT GCAGTGACTGGTGGACAGACAGTGGTCAACCCTTGGATTATCATCGGTGGGGTTGCCACTGTGGTGTGTC AGCAAAATGAATTCATAATGCCTGACAGCGCTGTGGTAGGAGATGTGCTGGTATTAACCAAGCCTTTAGG AACCCAGGTTGCTGCCAATGCCCACCAATGGCTGGATAATCCTGAGAAATGGAATAAAATCAAGATGGTG GTTTCCAGAGAGGAAGTAGAGCTAGCCTATCAGGAAGCTATGTTCAACATGGCTACTCTGAACAGGACTG CTGCTAGCTTGATGCACACTTTTAATGCTCACGCAGCCACGGATATCACAGGCTTTGGCATATTAGGACA CTCCCAGAACCTCGCAAAACAGCAAAAAAATGAAGTGTCCTTTGTCATTCACAATCTGCCAATCATTGCC AAGATGGCTGCGATCAGCAAAGCCAGTGGGCGCTTTGGCCTCCTCCAAGGAACATCAGCTGAAACCTCTG GGGGATTACTGATCTGTCTGCCAAGAGAACAGGCGGCCCGCTTTTGTTCGGAAATCAAATCTTCCAAGTA CGGAGAGGGTCACCAAGCTTGGATTGTTGGCATCGTGGAGAAGGGAAACCGGACAGCCCGGATCATTGAC AAGCCTCGCGTTATTGAAGTTCTACCTCGGGGAGCCTCTGCTTCTGCTGCTGCTGCTCCTGACAATTCCA ATGCAGCCTCTGAGCCTAGTTCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001079889
Insert Size	1356 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001079889.2, NP_001073358.2
RefSeq Size	2342 bp
RefSeq ORF	1356 bp
Locus ID	308993
Gene Summary	This gene encodes an enzyme that catalyzes the production of monoselenophosphate (MSP) from selenide and ATP. MSP is the selenium donor required for synthesis of selenocysteine (Sec), which is co-translationally incorporated into selenoproteins at in-frame UGA codons that normally signal translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is itself a selenoprotein containing a Sec residue at its active site, suggesting the existence of an autoregulatory mechanism. It is preferentially expressed in tissues implicated in the synthesis of selenoproteins and in sites of blood cell development. [provided by RefSeq, May 2017]

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