Agl (NM_001108564) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209001 representing NM_001108564 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACACAGTAAGCAGATTCGAATTTTACTATTGAACGAAATGGAGAAGCTAGAGAAGACCCTCTTCA GACTTGAGCAAGGATTTGAACTACAGTTCCGATTAGGCCCAACTTTACAAGGAAAAGCTGTTACTGTTTA TACAAATTACCCATTTCCTGGAGAAGCATTTAATCGAGAAAAATTCCGTTCCTTGGATTGGGAAAACCCA ACAGAGAGAGAAGATGATTCGGACAAATACTGTAAAGTTCATCTGCAGCAGTCTGGGTCTTTTCAGTACT ACTTCCTTCAAGGACAGGAGAAAAGTGGCGGAGGTTACATAGTTGTGGATCCTATTCTGCGAGTTGGAGC TGATAATCACGTGCTACCCTTGGATTGTGTTACTCTGCAGACATTTTTAGCAAAGTGTTTAGGACCCTTT GATGAGTGGGAAAGCCGACTACGGGTGGCAAAGGAGTCAGGCTACAACATGATTCACTTCACCCCGCTGC AGGCTCTCGGACTGTCCAGGTCATGCTATTCCCTTGCCGATCAGTTAGAATTAAATCCTGACTTCTCAAG GCCCAATAGAAGATACACCTGGGATGATGTTGGGCAGCTAGTAGAGAAGCTGAAGAGGGAGTGGAACATT CTTTGTATTACCGATGTGGTCTACAATCATACTGCTGCTAATAGTAAGTGGATCCATGAGCATCCAGAAT CTGCGTACAATCTTGTAAATTCTCCACACTTGAAACCTGCCTGGGTCCTAGACAGAGCGCTCTGGCATTT GTCCTGTGATGTTGCAGATGGGAAGTACAGAGAAAAAGGAGTCCCTGCTCTAATTGAAAATGACCATCAC ATGAACTGCATTCGGAAAATAATTTGGGAGGACATTTTCCCAAGGATTCAGCTCTGGGAGTTTTTCCAAG TAGATGTTCAGAAAGCGGTTGAACAATTTAGAAGACTGCTCTCTCAAGAAAACAGAAGAGTAACTAAGTC TGAACCGAAAGAACATCTTAAAATTATTCAGGACCCCGAATTCAGGCGGCTGGGCTGTGCTGTAGATATG AACATTGCTCTGGCGACCTTTATACCACATGACAATGGGCCAGCTGCAATTGAAGAGTGCTGTAATTGGT TCCGTAAGAGACTCGAGGAGTTAAATTCAGAGAAACACCACCTCACCAGCTGTCATCAGGAACAGGCCGT TAATTGTCTTTTGGGAAACGTGTTTTATGAACGGCTGGCGGGCCATGGCCCTAAACTAGGACCCGTCACC AGGAAGTACCCTTTAGTTACCAGGTATTTTACTTTCCCCTTTGAAGAAATGGCCTTGTCTGCAGAAGAGT CCCTGCTCCACCGCCCAGATAAAGCTTGCTTTCTGATGGCACACAATGGCTGGGTGATGGGCGATGACCC GCTTCGGAACTTTGCTGAGCCAGGTTCCAACGTTTATTTAAGGAGAGAACTTATTTGCTGGGGTGACAGT GTGAAATTGCGCTATGGGAACAAACCGGAGGACTGCCCTTACCTGTGGGCACACATGAAGAAATACACTG AGATCACTGCCACTCACTTCCAGGGAGTGCGCCTTGACAACTGCCACTCCACGCCTCTCCACGTGGCTGA GTACATGCTGGATGCTGCTAGGAAATTACAGCCCAATCTTTATGTAGTGGCTGAGCTGTTCACAGGAAGC GAAGATCTGGACAATATCTTCGTTACTAGACTGGGCATCAGTTCCTTAATAAGAGAGGCAATGAGCGCAT ATAACAGTCACGAAGAGGGCAGATTGGTCTACCGGTACGGAGGGGAGCCCGTCGGATCCTTCGTTCAGCC CTGTTTGAGGCCTCTTATGCCAGCTATCGCGCATGCCCTGTTTATGGACATCACCCACGATAATGAGTGT CCAATTGTGCACAGATCAGCCTATGATGCGCTTCCAAGTACCACAGTTGTCTCCATGGCGTGTTGTGCTA GTGGAAGTACAAGAGGCTACGACGAGCTAGTGCCTCATCAGATTTCAGTGGTTGCTGAAGAACGGTTTTA CACTAAGTGGAATCCTGGGGCATCCCCATCGGATACAGGAGATGTTAGCATCCACAGTGGGATTATTGCA GCCAGATGTGCCATCAACAGACTCCATCAAGAGCTTGGAGCCAAGGGTTTCATTCAAGTGTATGTGGATC AAGTGGATGAAGACATAGTGGCAGTAACCAGGCACTCACCAAGCATCCATCAGTCTGTTGTGGCTGTATC GAGAACGGCTTTTAGGAATCCCAAGACTTCATTTTACAGCAAAGAAGTGCCTCAAATGTGCATCCCTGGC AAAATTGAAGAGGTAGTTCTGGAAGCTAGAACTATTGAGAGGAATACAAAACCTTATAGGAAAGATGAGA ATTCAATCAATGGAATGCCGAACATGACAGTGGAACTTAGAGAACGTATCCAGCTTCATGAAAGTAAAAT TGTCAAACAAGCTGGAGTTGCCACAAAGGGTCCCAATGAATATATTCAGGAAATAGAATTTGAAAACCTA TCTCCAGGAAGTGTTATTATATTCAGAGTTAGCCTTGATCCACATGCGCAAGTTGCTGTTGGAATTCTTC GAAATCACCTGACCCAATTCAGCTCCCATTTTAAATCTGGAAGCCTTGCTGTTGACAACTCGGATCCTAT ATTAAAAATCCCCTTCGCTTCCATTGCCTCTAAATTGACTTTGGCTGAGCTAAATCAGGTACTTTACCGA TGTGAATCAGAAGAACAAGAAGACGGTGGAGGTTGCTATGATATACCAAACTGGTCGTCCCTTAAGTATG CAGGTCTGCAAGGATTAATGTCCGTGCTGGCAGAAATAAGACCAAAGAATGACTTGGGGCATCCCTTCTG TGAAAACTTGAGGTCTGGAGACTGGATGATCGACTACGTCAGTGGCCGGCTAATCTCACGATCAGGGAGT ATTGCTGAAGTTGGAAAATGGTTGCAGGCTATGTTCTTCTACCTGAAGCAGATCCCGCGATATCTCATCC CATGTTACTTTGATGCTATATTAATCGGTGCATATACCACTCTGCTGGATGTAGCCTGGAAACAGATGTC AAGCTTTGTTCAGAATGGCTCGACCTTCGTGAAGCACCTGTCCCTGGGTTCTGTCCAGATGTGTGGAGTC GGGAAGTGTCCTTGTCTGCCGCTGCTTTCACCCTCACTCCTGGATGTGCCGTGCAGGTTAAATGAGATCA CGAAGGAGAAAGAGCAGTGCTGTGCTTCCTTGGCTGCAGGCTTACCTCACTTTTCTTCTGGTCTTTTTCG CTGCTGGGGGAGGGATACTTTCATTGCACTTCGAGGGATGCTGCTGGTTACTGGTCGCTACCTAGAAGCC AGGAATATTATTTTAGCATTTGCTTGTACCCTGAGGCATGGTCTCATTCCTAACCTCCTTGGCGAAGGAA CGTATGCCAGGTACAACTGTCGTGATGCCGTGTGGTGGTGGCTGCAGTGCATTCAGGACTATTGCAAGAT GGTTCCAAATGGTCTAGACATCCTCAAATGCCCAGTTTCCAGAATGTATCCAACAGATGACTCTGCACCT TTGCCTGCTGGGACTCTGGATCAGCCTTTGTTTGAAGTCATACAGGAAGCTATGCAGAGGCACATGCAAG GCATTCAGTTCCGAGAGCGGAATGCCGGTCCACAGATCGATCGAAACATGAAGGATGAGGGTTTCAACAT AACCGTGGGGGTTGATGAAGAAACAGGATTTGTTTATGGAGGAAATCGCTTCAACTGTGGCACATGGATG GATAAAATGGGAGAGAGTGACAGAGCTAGAAACAGAGGGATCCCAGCCACCCCAAGAGATGGGTCTGCTG TGGAAATCGTGGGCTTGTGTAAATCTGCTGTTCGTTGGTTGCTGGAATTGTCCAAGAAAAACATTTTTCC TTATCATGAAGTCAGAGTAAGAAGACATGGAAAGGTTGTAGCAGTCTCCTATGATGAGTGGAACAGAAAA ATACAAAACAGTTTTGAAAAGCTCTTTCATGTTTCCGAAGACCCTTCAGATCCTAACGAGAAGCATCCGA ATCTGGTTCACAAGCGGGGCATCTACAAGGACAGCTACGGTGCGTCAAGTCCTTGGTGTGACTATCAGCT CAGGCCCAACTTTACCATAGCAATGGTCGTGGCCCCTGAGCTCTTTACTGCTGAAAAGGCTTGGAAGGCT TTGGAAATTGCAGAAGGAAAATTGCTTGGTCCCCTTGGCATGAAGACTTTGGATCCAGATGACATGGTTT ACTGTGGAGTCTATGACAATGCCTTAGACAATGACAACTACAACCTTGCCAGAGGTTTCAATTACCACCA AGGACCTGAGTGGCTATGGCCGATTGGGTATTTTCTTCGTGCAAAGTTGTATTTTTCCAAAATGATGGGC CCAGAGACTGCTGCGAAGACTGTATTTTTGGTTAAAAATGTTCTTTCTAGACATTATGTTCATCTTGAGA GATCCCCATGGAAAGGACTTCCAGAGCTCACCAATGAGAATGGCCAGTACTGCCCTTTCAGCTGTGAGAC ACAGGCATGGTCGATGGCTGCTGTTCTCGAAACCCTCTATGATCTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108564
Insert Size	4599 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108564.1, NP_001102034.1
RefSeq Size	7567 bp
RefSeq ORF	4599 bp
Locus ID	362029

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