Dagla (NM_001005886) Rat Untagged Clone

CAT#: RN204355

Dagla (untagged ORF) - Rat diacylglycerol lipase, alpha (Dagla), (10 ug)



  "NM_001005886" in other vectors (3)

CNY 16,820.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Nsddr
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN204355 representing NM_001005886
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCGGGATCGTGGTGTTCCGGCGGCGCTGGTCTGTGGGCAGCGATGACCTCGTGCTGCCTGCTATCT
TCCTCTTCCTGCTGCACACCACCTGGTTTGTGATCCTGTCTGTGGTGCTCTTCGGCCTGGTCTACAACCC
GCACGAGGCCTGCTCCCTGAACCTGGTGGACCACGGCCGAGGCTACCTGGGCATCCTTCTGAGCTGTATG
ATTGCTGAGATGGCTATCATTTGGCTGAGCATGCGTGGGGGTATCCTCTACACAGAACCCCGGGACTCCA
TGCAGTACGTGCTCTACGTGCGCCTGGCCATTTTGGTGATCGAGTTCATCTACGCTATCGTGGGCATCGT
CTGGCTTACACAGTACTACACCTCCTGCAATGACCTCACTGCTAAGAATGTCACTCTCGGGATGGTCGTC
TGCAACTGGGTGGTCATCCTCAGCGTCTGCATCACTGTCCTCTGTGTCTTTGACCCCACTGGCCGCACCT
TCGTCAAGCTGAGAGCCACCAAGAGGAGGCAGCGCAACCTGCGGACTTACAACCTGCGGCATCGGTTAGA
GGAGGGTCAGGCCACCAGCTGGTCCCGCCGTCTAAAAGTGTTCCTCTGTTGCACCCGGACAAAGGATTCC
CAGTCAGATGCCTATTCAGAAATTGCCTACCTCTTTGCTGAATTTTTCCGTGACCTCGACATAGTGCCCT
CCGACATCATTGCTGGCCTGGTGCTGCTTAGGCAGCGGCAGCGAGCCAAGCGCAATGCGGTGCTGGATGA
GGCAAACAATGACATCTTGGCTTTCCTGTCTGGGATGCCAGTGACCAGAAACACCAAGTACCTCGACCTC
AAGAACTCGCACGAGATGCTACGCTACAAAGAAGTCTGCTACTACATGCTCTTTGCCCTCGCTGCCTATG
GCTGGCCCATGTACCTGATGAGGAAGCCCACCTGTGGCCTCTGCCAGCTGGCTCGGTCCTGCTCGTGTTG
TCTCTGCCCTGCACGCCCCCGATTCGCCCCTGGGGTCACCATTGAGGAAGACAACTGTTGTGGCTGCAAT
GCAATTGCCATCCGCCGTCACTTCCTGGATGAGAACATGACTGCTGTAGACATCGTCTATACCTCCTGTC
ATGATGCGGTCTATGAAACTCCCTTCTACGTAGCTGTGGACCATGACAAGAAGAAGGTGGTGATCAGTAT
CCGGGGAACACTGTCCCCCAAGGATGCCCTGACTGACCTGACAGGAGATGCTGAGCGTCTCCCTGTGGAG
GGACACCGAGGCACCTGGTTGGGCCACAAGGGCATGGTACTCTCAGCTGAGTACATCAAGAAGAAGCTGG
AGCAGGAGATGGTCCTGTCCCAGGCCTTTGGGCGAGACCTGGGCCGTGGAACCAAACACTATGGCCTGAT
TGTGGTAGGCCACTCCTTGGGAGCAGGCACAGCCGCCATTCTCTCCTTCCTCCTGCGCCCTCAGTACCCG
ACCCTCAAGTGCTTCGCCTACTCCCCTCCAGGAGGCCTGCTGAGTGAGGATGCCATGGAGTACTCCAAAG
AGTTTGTGACTGCTGTGGTTCTGGGCAAAGACCTTGTTCCCAGGATCGGCCTTTCCCAGCTCGAAGGTTT
CCGCCGGCAGCTTCTGGATGTCCTGCAGCGAAGCACCAAGCCCAAATGGCGGATCATCGTGGGGGCCACC
AAGTGTATCCCCAAGTCGGAGCTGCCTGAGGATCAGGTGGAAGTGACTGCCTTGGCCAGCACACGGCTCT
GGACACACCCCAGTGATCTGACCATCGCCCTTTCAGCCAGCACCCCACTCTACCCACCTGGCCGTATCAT
CCATGTGGTCCATAACCACCCTGCTGAACAGTGCTGCTGCTGTGAACAGGAAGAGCCCACATACTTTGCC
ATCTGGGGTGACAACAAAGCCTTTAATGAGGTGATCATCTCACCAGCCATGCTCCATGAGCACCTGCCCT
ACGTGGTCATGGAGGGGCTCAATAAGGTGCTGGAGAATTACAACAAGGGAAAGACAGCGCTGCTCTCTGC
TGCAAAGGTCATGGTGAGCCCCACTGAGGTAGACCTCACTCCTGAGCTCATCTTCCAGCAACAGCCGCTG
CCCACGGGGCCACCTCTGCCCACTGGGCTGGCTCTGGAGCTGCCTGCCACAGAGCATCGCAACAGCAGTG
TCAGGAGCAAGTCTCAGTCTGAGATGAGTCTAGAGGGCTTCTCTGAAGGACGGCTGCTGTCCCCAGTGGC
TGCTGCCTCAGCAGCCCGGCAAGACCCTGTAGAGCTGTTGCTGCTGTCCACCCAGGAACGACTGGCCGCG
GAGCTGCAGTCCCGGCGGGCACCACTGGCCACTATGGAGAGCCTCTCAGACACTGAGTCACTGTACAGCT
TTGACTCACGCCGTTCCTCAGGCTTCCGCAGCATCAGAGGCTCACCCAGCCTCCATGCAGTACTGGAGCG
TGATGAGGGCCACCTGTTTTACATCGACCCAGCCATTCCAGAGGAGAACCCATCCCTCAGCTCCCGCACA
GAGCTGCTGGCAGCTGACAGTCTGTCCAAGCACTCGCAGGACACCCAACCCCTGGAGGCAGCACTAGGCA
GCGGGGGTGTGACTCCTGAGCGACCACCAAGTGCAGCCAATGACGAGGAGGAGGCAGCCGGTGGCAGTGA
GGGTGGTGGGGTGGCCCCACGAGGAGAGCTGGCACTGCACAATGGGCGCCTGGGGGATTCGCCCAGCCCT
CAGGTGCTAGAATTCGCTGAGTTCATTGACAGCCTCTTCAACCTGGACAGCAAGAGCAGCTCCTTCCAGG
ACCTGTACTGCATGATGGTGCCTGAGAGCCCCACTAGTGACTACACAGAGGGCCCCAAGTCCCCCAGCCA
ACAGGAAATCTTGCTTCGGGCTCAGTTTGAGCCCAATCTGGTACCCAAGCCCCCCAGGCTCTTTGCAGGA
TCTGCCGAACCCTCCTCTGGCATCTCACTCTCACCCTCCTTCCCACTCAGCTCCTCAGGGGAGCTCATGG
ACCTGACACCCACGGGCCTCAGCAGCCAGGAGTGTCTGGCCACAGACAAGATCCGGACTTCCACCCCCAC
AGGCCATGGGGCCAGCCCTACCAAGCAGGATGACCTGGTCATCTCAGCACGCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001005886
Insert Size 3135 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001005886.1, NP_001005886.1
RefSeq Size 5568 bp
RefSeq ORF 3135 bp
Locus ID 309207
UniProt ID Q5YLM1
Gene Summary Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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