United States
Germany
Japan
United Kingdom
China
Trmt12 (NM_001122976) Rat Untagged Clone
CAT＃: RN202513
Trmt12 (untagged ORF) - Rat tRNA methyltransferase 12 homolog (S. cerevisiae) (Trmt12), (10 ug)
CNY 5,610.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | RGD1307127 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN202513 representing NM_001122976
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGAGAATGTGAGAAGTCTGTAGTTGTCGCAGTTGTGACTGAGCCTCGGTTTACCCAGAGATACA GAGACTATCTCGAGAAGCAGAAACTCTTGGATAGACAGTACCGTGTGGAAAAGTTGCGGGATGGCACGGT GGCGCTACCGGTGCTGGCAGAAACCCTGTCTGAACATCATCTGCAGGAGCTGAAGAATCGCGTGGCCCCC GGCAGCACCTGTAAGCTGACGCAGCTCCTGGATCCTCTTCCTTCTAAAAAGGCCCGGGTTTGTTCACCTG CCCAAAGACTGTGTCTTGAGGTGAGGCGTTGGGTAGAGGACCGCGGAGTGACGTGGTCCACTGAATTGGA GGCAGATTTGCCCCGGTCTTGGCAACGGCATGGTGACCTAATGCTGCTTAGTGAAGACTGTTTCCAAGCC ACGCAGTGGAAAAGGCTGGAACCAGAACTCTGGGAGACTGTTGCCTCAGCCCTTGGAGTTCAGCGTTTGG CAAAACGAGGGCGGGTGCTACCTGATGGTACTCGAACTCCGACAGTGACTCTGCTGCTCGGTGACCATGG CTGGGTAGAGCATGTAGATAATGGCATCCGGTATAAGTTTGATGTGACACAGTGCATGTTCTCCTTCGGA AACATCACTGAGAAACTTCGAGTGGCCTCACTCTCCTGTGCTGGAGAAGTGCTGGTGGATCTCTATGCAG GGATTGGTTATTTTACGTTGCCCTTCCTAGTTCATGCTGGCGCCGCTTTCGTCCATGCTTGTGAATGGAA TCCTCATGCAGTAGTTGCTCTAAGAAAGAATTTGGAGATCAATGGAGTGGCAGACCGGTGCCAAATACAT TTTGGGGACAACAGGAAACTGAAACTCTCAAACACTGCAGATAGGGTGAACCTGGGGCTCATTCCCAGTT CTGAAGAAGGCTGGCCCATTGCCTGCCAAGTGCTGCGAAAGGATGTTGGGGGTATTCTGCATATCCACCA AAATGTGGAATCTTTCTCGGGAAAGAATCCACAGCCACCTGGAAGCAGTAACATGGAAAAGAAGCATTGG CCTCATCCCCAGAAAATTACCACTGATAAACAGGGAAATAGAACTACTGGAAGTTGCATGGGGGAAATGT CATCAGCCAGCAAACCAGAGTGGCAAAGATGGGCAGAATCTGCAGAAAGTCAGATCGCCTCTCTTCTTCA TCAAGTACATGGGAAACCATGGAGGACACGAATCCTACATGTCCACCCAGTGAAATCTTACGCCCCCCAC GTGGATCACATAGTCCTAGATCTGGAATGCCGCCCCTGCCCTCTAGTTGGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001122976 |
| Insert Size | 1314 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001122976.2, NP_001116448.1 |
| RefSeq Size | 1655 bp |
| RefSeq ORF | 1314 bp |
| Locus ID | 314999 |
| UniProt ID | Q4V8B8 |
| Gene Summary | S-adenosyl-L-methionine-dependent transferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14) to produce wybutosine-86 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR202513 | Trmt12 (Myc-DDK-tagged ORF) - Rat tRNA methyltransferase 12 homolog (S. cerevisiae) (Trmt12), (10 ug) |
CNY 5,610.00 |
|
| RR202513L3 | Lenti ORF clone of Trmt12 (Myc-DDK-tagged ORF) - Rat tRNA methyltransferase 12 homolog (S. cerevisiae) (Trmt12), (10 ug) |
CNY 7,510.00 |
|
| RR202513L4 | Lenti ORF clone of Trmt12 (mGFP-tagged ORF) - Rat tRNA methyltransferase 12 homolog (S. cerevisiae) (Trmt12), (10 ug) |
CNY 8,360.00 |
