Hr (NM_024364) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202331 representing NM_024364 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGTATGCCCAGCTTCCTGAAGGACACCCCAGCCTGGGAGAAGACTGCCCCTGTGAACGGCATTG TGGGACAGGAGCCTGGTACCTCCCCGCAGGATGGCTTGCACCATGGGGCACTGTGCCTAGGAGAACCTGT TCCCTTTTGGAGGGGTGTTCTGAGCGCCCCAGACTCCTGGCTCCCTCCTGGCTTCCTCCAAGGCCCCAAG GACACACTCTCAGTGGTGGAGGGTGAGGGTTCTCGGAATGGGGAGAGGAAAGCCAACTGGCTGGGTAGCA AGGAGGGACTACGCTGGAAGGAAGCGATGCTGGCCCATCCACTGGCCTTCTGTGGGCCAGCATGCCCACC TCGCTATGGCCCCCTGATACCTGAGCATAGCAGTGGCCATCCCAAGAGTGACCCTGTGGCCTTCCGGCCC TTGCACTGCCCTTTCCTGCTGGAGACCAAGATTCTAGAGCGAGCTCCCTTCTGGGTTCCTACCTGCTTGC CTCCCTACCTGATGTCCAGCCTGCCTCCAGAGCGTTCGTATGACTGGCCTTTGGCCCCAAGCCCCTGGGT ATACTCTGGGAGCCAGCCAAAAGTGCCCTCTGCCTTCAGCTTAGGCAGCAAGGGCTTTTACCACAAGGAT CCAAACATCCTCAGGCCAGCAAAGGAGCCCTTGGCAGCGTCAGAGTCTGGGATGTTGGGCTTAGCTCCTG GAGGGCATCTCCAGCAAGCCTGCGATGCAGAAGGCCCCTCGCTTCACCAGAGGGATGGGGAGACAGGAGC TGGCAGGCAGCAGAATCTTTGCCCAGTTTTCTTGGGGTACCCAGACACTGTTCCTCGAACCCCCTGGCCT TCTTGTCCCCCAGGCCTGGTTCACACTCTCGGTAACGTTTGGGCTGGGCCAGGAAGTAACAGCTTTGGGT ACCAGCTAGGCCCACCAGTCACACCAAGGTGCCCATCACCTGGGCCTCCCACCCCTCCAGGGGGCTGTTG CTCATCCCATCTGCCTGCCAGAGAGGGTGATCCTGGCCCTTGTAGGAAATGTCAGGATAGCCCAGAGGGA AGTAGCAGTGGGCCAGGGGAATCCAGTGAAGAAAGAAACAAGGCTGGCTCCAGGGCCTCTCCTCCAAGCC ATCACACCAAGCTGAAAAAGACCTGGCTCACACGCCACTCGGAACAGTTCGAGTGCCCAGGTGGCTGTCC TGGGAAGGGAGAGAGCCCAGCCACTGGGCTCCGGGCACTCAAGAGGGCAGGCAGTCCAGAGGTCCAGGGA GCAAGGGGCCCGGCTCCCAAACGCCCATCCCACACTTTCCCAGGTACTGGGAGGCAGGGGGCCAGGGCTT GGCAGGAGACACCAGAGACATCCACAGGAAGCAAGGCAGAGGCACAGCAACAAGAGGAGCAAAGAGGGCC CCGAGATGGCAGGATTCGGCTCCGGGAATCCAGACTTGAGGATACATCCTGCCAGCATCACTTAGCAGGT GTCACTCAGTGCCCAAGCTGTGTCCAGGCAGCTGGAGAGGTGGAGATACTGACCAGCCACTCCCAGAAAT CACATAGGTTACCCCTGGAAGAGAAGCCGTTGGAGGAGGATTCCTGTGCCACTTCTGAGGAAGGAGGAGG GTCTAGCCCAGAAGCTTCCATCAACAAGGGCCTAGCCAAGCATCTACTGAGTGGTCTGGGTGACCGACTC TGCCGCCTGCTGCGGAAGGAGCGGGAAGCCCTCGCCTGGGCACAGCGTGAAGGCCAGGGGCCAGCCATGA CAGAGGACAGCCCAGGCATTCCACACTGCTGCAGCCGTTGCCACCACGGACTCTTCAACACCCACTGGAG ATGTTCCCACTGTAGCCACCGGCTGTGTGTAGCCTGTGGTCGCATAGCCGGAGCTGGGAAGAACAGGGAG AAAACAGGCTCTCGGGAGCAGCGCACAGATGACTGTGCCCAGGAGGCTGGGCATGCTGCCTGTTCCCTGA TTCTGACCCAGTTTGTCTCCAGCCAGGCGCTGGCAGAACTGAGCACTGTGATGCACCAAGTCTGGGCCAA GTTTGACATTCGGGGGCACTGTTTCTGTCAAGTCGATGCCCGTGTGTGGGCCCCCGGGGATGGGGGTCAG CAGAAGGAACCAACAGAGAAAACTCCCCCAGCTCCACAACTTTCCTGCAATGGAGATTCCAATCGGACCA AGGACATCAAAGAAGAGACCCCAGACTCCACAGAGAGCCCAGCAGAGGACCGTGCTGGCCGGTCACCACT TCCTTGTCCCTCTCTTTGTGAGCTGCTAGCCTCTACAGCTGTCAAACTCTGCCTGGGGCATGAGCGGATT CACATGGCCTTTGCCCCGGTCACCCCGGCTCTGCCCAGTGATGACCGCATTACCAACATCCTGGACAGCA TTATTGCGCAGGTAGTAGAACGGAAGATCCAAGAGAAAGCCCTGGGGCCAGGCCTTCGAGCAGGGTCAGG CTTACGAAAGGGCCTCAGCCTGCCCCTGTCACCAGTGCGAACCCGGTTGTCTCCTCCTGGAGCTCTGCTG TGGCTGCAAGAGCCCAGGCCTAAGCACGGCTTTCGTCTCTTCCAGGAGCACTGGCGGCAGGGCCAGCCTG TGTTAGTATCAGGCATCCAGAAGACATTGAGACTTAGCCTGTGGGGAATGGAAGCCCTTGGGACACTTGG TGGCCAGGTGCAGACACTGACTGCCCTTGGGCCGCCCCAACCCACCAGCCTGGACAGCACAGCATTCTGG AAGGGATTCTCTCATCCTGAGGCACGTCCAAAGTTAGATGAGGGCTCTGTCCTCCTGCTACACCGACCCC TGGGGGATAAGGACGAGAGCAGGGTGGAGAACCTTGCCTCCAGCCTTCCACTCCCAGAGTACTGTGCCCA CCAAGGGAAACTCAACCTAGCGTCCTACCTCCCCCTGGGTCTCACACTGCATCCACTGGAGCCCCAGCTC TGGGCAGCCTATGGTGTGAACTCGCACCGTGGACACCTGGGGACCAAGAACCTATGTGTGGAGGTGTCTG ACCTAATCAGTATCCTGGTGCATGCTGAGGCACAGCTGCCTCCCTGGTACCGAGCACAGAAAGATTTTCT CTCAGGCCTGGATGGGGAAGGGCTCTGGTCTCCAGGTAGCCAGACCAGCACTGTGTGGCATGTGTTCCGA GCCCAGGATGCCCAGCGTATCCGTCGCTTTCTCCAGATGGTGTGCCCAGCTGGAGCAGGAACCTTGGAGC CTGGTGCCCCAGGCAGCTGCTACTTGGATTCAGGGTTGCGACGCAGGTTAAGGGAGGAGTGGGGTGTGAG CTGCTGGACACTGCTGCAGGCTCCTGGAGAAGCGGTGCTGGTGCCTGCTGGGGCACCCCATCAGGTACAG GGCTTGGTGAGCACAATCAGTGTCACTCAGCACTTTCTGTCTCCTGAGACCTCGGCCCTCTCTGCTCAGC TCTGCCACCAGGGAGCCAGCCTGCCCCCTGACCATCGTATGCTTTATGCCCAGATGGACCGGGCTGTGTT CCAAGCAGTGAAGGTGGCGGTGGGGACATTACAGGAAGCTAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_024364
Insert Size	3546 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_024364.3, NP_077340.3
RefSeq Size	5617 bp
RefSeq ORF	3546 bp
Locus ID	60563
Gene Summary	This gene encodes a protein that is involved in hair growth. This protein functions as a transcriptional corepressor of multiple nuclear receptors, including thyroid hormone receptor, the retinoic acid receptor-related orphan receptors and the vitamin D receptors, and it interacts with histone deacetylases. The translation of this protein is modulated by a regulatory open reading frame (ORF) that exists upstream of the primary ORF. Mutations in this upstream ORF cause Marie Unna hereditary hypotrichosis (MUHH), an autosomal dominant form of genetic hair loss in human. [provided by RefSeq, Oct 2014]

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