Tnn (NM_001107189) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN201535 representing NM_001107189 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCTGTGGGGGATGCTTGCCTTCCCCCTGGGATTCCTGCTTGCTTCTGCGCTCCTGGTGGCTTCAG CCCCAGCCAACCCAGAGACTCCTGGCTGCAGCCACAAAGACCAACAGGTCACCGTTAGCCACACCTACAA GATTGATGTGCCCAAGTCTGCTCTGGTTCAAGTAGAGACGGACCCACAGTCACTCAGTGATGATGGGACA TCACTCTTGGTTCCTGGGGAGGATGGGGAGGAGCAGAACATTATCTTCAGGCACAACATCCGTCTTCAGA CACCGCAGAAGAACTGTGAGTTGGCCGACAGTGTCCAGGACCTGCTAGCCCGGGTGAAAAAGCTGGAGGA GGAGATGGCAGAGCTGAAGGAGCAGTGTAGTACCAGCCGCTGCTGCCAGGGAGCTGCCGATCTGAGCCGT CACTGCAGTGGGCATGGGACCTTCTTCGCTGAGACCTGCAGCTGCCACTGTGACCAGGGCTGGGAGGGCG CTGACTGTGAGCAGCCCACCTGTCCTGGGGCTTGCAGCGGCCACGGGCGGTGCGTGGATGGGCAATGCGT GTGTGATCAGCCCTATGTGGGGGTCGACTGCGCCTACGCCGCCTGTCCCCAGGACTGCAGTGGGCATGGC GTGTGTGTGCGGGGTGTCTGCCAGTGCCACAAGGACTTCACAGCTGAGGACTGCAGCGAGCAGCGCTGTC CTGGCGACTGTAGTGGCAACGGCTTCTGTGACACTGGCGAGTGTTACTGCGAGATGGGCTTTACTGGCCC CGACTGTTCCCAGGTGGTGGCTCCTCAGGGCCTGCAGTTGCTCAAGAGCACCGAGAACTCCCTGCTGGTG AGCTGGGAGCCCTCCAGTGAGGTGGACCACTACCTGCTTAGCTACTACCCCTTGGGGAAGGAGCAAGCTA CAAAACAGGTCCGGGTGCCCAAGGAGCAACACACCTACGACATCACTGGTTTGCTGCCTGGCACCAAGTA CATAGTCACCCTGCGTAACGTGAAGAAGGACATTTCCAGCAGCCCTCAGCATCTCCTTGCCACCACAGAT CTTGCTGTGGTGGGCACGGCCTGGGTAAATGAGGAGACTGAGACATCTCTTGATGTGGAGTGGGAGAACC CTCTGACTGAGGTGGACTATTACAAGCTTCGGTATGGCCCCTTAACAGGGCAGGAAGTGACTGAGGTCAC TGTGCCCAAGAGCCGTGATCCCAAGAGTAGATATGACATCACCGGTCTGCAGCCTGGAACGGAATATAAA ATCACAGTTGTGCCCATAAGAGGAGATCTGGAGGGAAAGCCAATTCTCCTCAATGGCAGGACAGAAATTG ATGGACCAACCAATGTGGTCACAAACCAAGTGACAGAAGACACAGCATCTGTTTCCTGGGATCCAGTGAG GGCTGACATAGACAAGTATGTGGTGCGCTATATCTCCCATGATGGGGAGACCAAGGAGAAGGCAGTACCC AAGGACCAGAGCAGCACCGTTCTCACGGGCCTGAAGCCAGGAGAGGCCTACAAAGTCTTTGTGTGGGCTG AGAGAGGCAACCAAGGCAGCAAGAAAGCAGACACCAAGGCCCTCACAGAAATTGACAGCCCAGAAAACCT GGTGACCGACCGTGTGACAGAGAACAGTCTCTCTGTCTCGTGGGACCCGGTGGAGGCTGAAATAGACAGG TACGTGGTGAGCTACACTTCCGTGGATGGAGAGACAAAGCAGGTTCCAGTGAGGAAGGACCAAAGCAGCA CCGTCCTCACCGGCCTGAGTCCAGGTGTGGAGTACAAAGTGTACGTGTGGGCAGAGAAAGGTGATCGAGA GAGCAAGAAGGCCAACACCAAGGCTCCCACAGACATTGACAGCCCCAAAAACTTGGTGACTGACCAGGTG ACAGAGAACACTCTCAGTGTCTCTTGGGACCCTGTTCAGGCCAACATTGACAGGTATATGGTGAGCTACA CTTCTGCTGATGGAGAGACCAGGGAGGTTCCAGTGCCTAAGGAGAAGAGCAGCACTGTTCTGACAGGCCT GAGGCCAGGCATGGAGTACAAGGTTCACGTATGGGCCCAGAAGGGGACCCAGGAGAGCAGGAAGGCCAAT ACCAAGGCCCCCACAGATATTGATGGCCCCAAAAACCTAGTGACTGACCAGGTGACAGAGACCACTCTTA GTGTCTCCTGGGACCCAGTGGAGGCTGACATCGATAGATACATGGTACGCTACACGTCTCCTGATGGAGA GACCAAGGAGGTCCCTGTGTCAAAGGATAAGAGCAGCACAGTCCTGAGGGGCCTGAGGCCAGGTGTGGAG TACAAGGTTGATGTGTGGGCCCAGAAGGGGGCCCAGGACAGCCGGAAGGCCAACACCAAGGCCCCCACAG ATATTGACAGTCCCAAAAACCTCGTGACTGAGCAGGTGACAGAGAGCACTGCCACCGTGTCCTGGGACCC AGTAGAGGCTGACATTGACAGGTATGTGGTGCGCTACACCTCTGTCGATGGAGAGACCAGGGAATTTCTC GTTGGGAAGGATCAAACAAGCACCATCCTGACCGGCATGAAACCGGGTGTGGAGTATCAGGTCGATGTGT GGGCCCAGAAGGGGACACGGGAGAGCAGGAAGACCAGCACCAAGGCCCCAACAGATATTGATGGCCCCAA AAACCTAGTGACTGACCAGGTGACAGAGACCACTCTTAGTGTCTCCTGGGACCCAGTGGAGGCTGACATC GATAGGTACATGGTACGCTACACGTCTCCTGATGGAGAGACCAAGGAGGTCCCTGTGTCAAAGGATAAGA GCAGCACAGTCCTGAGGGGCCTGAGGCCAGGTGTGGAGTACAAGGTTGATGTGTGGGCCCAGAAGGGGGC CCAGGACAGCCGAAAGGCCAACACCAAGGCCCCCACAGATATTGACAGTCCCAAAAACCTGGCGATCAAC CAGGTGACAGAGACCACGCTCAGTGTCTCCTGGGACCCAGTACAGGCTGATATCGACAGGTATGTGGTGC GCTACACCTCTGCTGATGGAGAATCCAAAGAATTCTTGATTGGGAAGGAACAGAGGAGCACTGTCCTGAC AGGCCTGAGGCCAGGCGTAGAGTACAAGGTTGAAGTGTGGGCCCAGAAAGGAGCCCGGGAGAGCAAGAAA GCCAACACCAAAGGCCACACAGACATTGACAGTCCCAAAAACCTGGTGACCAATCAGGTGACAGAAAATA CAGCCACCATCTCCTGGGACCCAGTGCAGGCTGATATTGACAGGTACATGGTGCGCTACACCTCTGCTGA TGGAGAGACCAGGGAGGTTCCAGTGAGGAAGGAGAAGAGTAGCACGGTGCTCACAGGCCTGAGGCCAGGA GTGGAGTACACAGTCCAAGTGTGGGCTCAGAAGGGGGCCCGGGAGAGCAGGAAGGCCAAAACCAAGGCCC CCACAGAAATTGACAGCCCCAAGAACTTGGTGACCAACCGAGTGACAGAGAATACAGCCACCATCTCCTG GGACCCAGTACGAGCCACCATTGACAGGTACATGGTTCGCTACACCTCTGCGGATGGAGAGACTAAGGAG GTTCCAGTGTCAAAGGACCAGAGTAGCACCATCCTGACAGGTCTGAAGCCAGGCATGGAGTATACCATTC ATGTGTGGGCCCAGAAGGGGGCTCGGGAGAGCAAGAAGGCTGATACCAAGGCCCTAACAGAAATTGACCC TCCCAAAAATTTCCGTCCATTTGGTGTAACACATTCCAGTGGGGTTTTGACCTGGATGCCCCCCTCTGCT CAAATCGATGGCTACATTTTGACCTACCAGCTTCCAAATGGCATCTTGAAGGAGGTGGAGCTCCAAAGAG GCCAGCAGAGATTTGAGTTGCAAGACCTGGAACAGGGCGTCACCTATCCTGTTTCTCTGGTTGCCTTTAA AGGTAATCAGCGGAGCCGGAGTGTGTCTACCACCCTTTCTACAGTGGACGCCCGATTTCCACACCCCTCA GACTGCAGTCAGGTTCAGCAGAACACCAATGCTGCTGCCAGTGGCCTCTACACGATCTACCTCAATGGCG ATGCCAGCCGGCCCATGCAGGTTTATTGTGACATGGACACGGATGGAGGCGGCTGGATTGTCTTTCAGAG ACGTAACACTGGGCAGCTGGATTTCTTCAAGCGTTGGCGGAGTTATGTAGAAGGCTTTGGGGACCCCATG AAGGAGTTCTGGCTTGGACTTGACAAACTACATAATCTCACCACTGGCACTACCACTCGGTATGAGGTGA GGGCAGATTTACAGACTGCCAATGAATCCGCCTACGCTGTGTATGACTTCTTCCAAGTGGCATCCAGCAA AGAGCGGTACAAGCTGTCGGTTGGGAAATACAGAGGCACAGCAGGGGATGCTCTTACCTACCACAATGGT TGGAAGTTCACAACTTTTGACAGAGACAATGATATTGCCCTCAGCAACTGCGCGCTGACGCATCATGGCG GCTGGTGGTATAAGAACTGCCATTTGGCCAACCCGAATGGCAAATATGGGGAGACCAAGCACAGTGAGGG GGTGAACTGGGAGCCATGGAAAGGACATGAGTTCTCCATTCCTTACGTGGAGCTAAAAATCCGCCCCTTT GGTTACAGCAGAGACCGTTTCTCTGGCAGGAAGAAGCGGTCCATAGGAGGGAAGGCAAGAGTGTTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107189
Insert Size	4689 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107189.2, NP_001100659.2
RefSeq Size	5783 bp
RefSeq ORF	4689 bp
Locus ID	304913

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