Sptb (NM_212522) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Spnb1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200996 representing NM_212522 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACATCAGCCACGGAGTTTGAAAACGTGGGCAACCAGCCACCATACAGCCGGATTAATGCCCGCTGGG ACGCCCCAGATGATGAACTGGACAACGATAGCAGCTCAGCCAGGCTCTTTGAGAGGTCCCGGATCAAGGC CTTGGCAGATGAGCGGGAAGTGGTTCAGAAGAAAACCTTTACGAAATGGGTGAACTCCCACCTGGCTCGA GTGTCCTGCCGCATCAGCGATCTCTACAAGGACCTGCGGGATGGACGCATGCTCATCAAGCTGCTGGAGG TGCTCTCCGGAGAGATGCTGCCAAGGCCGACCAAGGGCAAGATGCGCATCCACTGCCTGGAGAACGTGGA CAAAGCCCTGCAGTTCCTCAAGGAGCAGCGCGTGCACCTGGAAAACATGGGCTCCCACGACATCGTGGAT GGCAACCACCGCCTGGTCCTGGGCCTCATCTGGACGATTATCCTCCGATTCCAGATTCAAGACATTGTTG TCCAAACTCAAGAAGGTCGGGAGACACGCTCAGCCAAGGATGCTCTGCTTCTGTGGTGCCAGATGAAGAC AGCCGGCTACCCCCAAGTCAACGTAACCAACTTCACCTCCAGCTGGAAGGACGGCCTGGCCTTCAATGCC CTAATTCATAAACACCGGCCTGACCTCATTGACTTTGACAAGCTAAAGGACTCCAATGCCCGGCATAACT TGGAGCACGCATTTGATGTGGCCGAGCGTCAGCTGGGCATCATCCCACTCCTTGACCCCGAAGATGTCTT CACAGAAAACCCAGACGAGAAGTCCATCATCACCTACGTGGTAGCATTTTACCACTACTTCTCCAAGATG AAGGTGCTAGCGGTGGAGGGCAAGAGAGTCGGCAAGGTCATTGACCATGCCATTGAGACTGAGAAGATGA TTGAAAAGTACAGTGGGCTAGCCTCAGACCTGCTCACCTGGATTGAGCAAACCATTAGCGTCCTGAACAG CCGCAAGTTTGCCAACTCATTGAGTGGCGTCCAGCAGCAGCTACAGGCCTTCAGCACCTACCGCACTGTG GAGAAGCCACCCAAGTTTCAAGAGAAGGGGAATCTGGAAGTCCTCCTGTTCACTATCCAATCCCGAATGA GAGCCAACAATCAGAAAGTGTACACGCCCCACGATGGGAAGCTAGTGTCTGACATCAACAGGGCCTGGGA GAGCCTAGAGGAAGCAGAATACCAAAGGGAGCTGGCCCTGAGGAGCGAGCTCATTCGGCAGGAGAAACTG GAGCAGCTGGCGCGGCGCTTCGACCGTAAGGCAGCCATGAGAGAGACGTGGCTCAACGAAAACCAGCGCC TGGTGACCCAGGATAACTTCGGGTATGACCTGGCAGCTGTGGAGGCCGCCAAGAAGAAGCACGAAGCCAT TGAGACCGACACGGCTGCCTATGAGGAGCGGGTGAAGGCACTGGAGGACCTGGCCCAGGAGCTGGAGAAG GAGAATTACCATGACCAGAAGCGCATCAGTGCCAGGAAGAACAATATCCTCCGTCTGTGGAGCTACCTGC AGGAGCTGCTGCGATCCCGGCGACAGCGGCTCGAGGCCACCCTGGCACTGCAGAAGCTCTTCCAGGACAT GCTGCACAGTATCGACTGGATGGATGAGATCAAGGCGCACATCTTGTCTGCTGAATTTGGGAAGCACTTG TTAGAGGTTGAAGACCTGCTACAGAAACACAAGCTAATGGAAGCCGACATTGCTATCCAAGGGGACAAAG TGAAGACCATCACCGCGGCCACCCTGCAGTTCACTGAGGGGAAAGGGTATCAGCCTTGTGACCCCCAGGT CATCCAGGACCGTGTCAGCCACCTAGAGCAATGCTTCAGTGAACTGAGCAACATGGCAGCTGGACGGAAG GCTCAGCTGGAGCAGTCTAAGCGACTCTGGAAGTTCTTCTGGGAGATGGACGAGGCCGAAAGCTGGATCA AGGAAAAGGAACAGATCTACTCCTCCCTGGACTACGGCAAGGACCTGACGAGTGTGCTCATCTTACAGCG CAAGCACAAGGCCTTCGAGGACGAGCTCCGTGGGCTCGATGCCCATCTGAAGCAGATCTTCCAGGAGGCT GAGGACATGGTTGCACAAAAGCAGTTTGGACATCCACAGATCGAGACCCGTGTCAAAGAAGTATCGGCTC AGTGGGACCACTTGAAAGAGCTGGCTGCCTTTCGTAAGAAAGACCTGCAGGATGCGGAAAACTTCTTCCA GTTCCAGGGTGATGCAGACGACCTGAAGGCTTGGCTGCAAGACGCTCACCGGCTGCTTTCCGGTGAAGAT GTGGGGCAAGACGAAGGAGCCACGAGGGCCCTGGGAAAGAAGCACAAGGAGTTTCTAGAGGAGCTGGAGG AGAGCCGCGGGGTGATGGAGCACTTAGAGAACCAAGCCCAGGGCTTCCCCGAGGAGTTTCGAGATTCCCC AGATGTGACAAACCGTCTACAGGCACTCAGGAAGCTCTACCAGCAGGTGATGGCCCAGGCAGAATTGCGT GGACACAAGCTGCAGGAAGCCCTGGACTTGTACACTGTGTTTGGAGAGTCAGACGCCTGTGAGCTGTGGA TGACTGAGAAGGGGAAGTGGCTGGACCAGATGGATATCCCAAACACGCTGGAGGATCTGGAGGTTGTGCA GCACAGGTTTGACATCCTGGACCAGGAGATGAAGACCTTGATGGCGCAGATTGATGGCGTGAACCTTGCG GCCAACAACCTGGTAGAGAGCGGCCACCCACGCAGTGGGGAGGTGAAACAATATCAGGACCGCCTAAACA AGAGGTGGCAGGCCTTCCAGGCAGTGGTGTCAGAGCAGAGAGAGGCTGTGGACTCGGCTCTCCGAGTAAA CAATTACTGTGTAGACTGTGAGGAAACCAGCAAGTGGATCGTGGACAAGACGAAGGTTGTGGAGTCTACC AAGGACTTGGGGCAGGACCTAGCAGGAGTCATTGCCATCCAGCGGAAGTTGTCAGGGTTGGAGCGGGACG TATTGGCCATCCGGGATCGTGTGAGCGCCTTGGAACGGGAGTCGCAGTACTTGATGGAGTCACACCCTGA CCAGAAAGAGGACATTGGCCAGCGGCAAGCAGATGTCGAGAAGATGTGGAAGGGCCTGCAAGATGCCTTG CAAGGTCAGGAGCTCTCCTTAGGTGAGGCCAGCAAGCTGCAGGCTTTCCTACAAGACCTGGATGACTTCA AGGCCTGGCTGTCGATGGCCCAAAAGGCAGTGGCCTCTGAGGACATGCCTGAGTCACTCCCAGAGGCTGA GCAACTCCTACAGCAGCATGCAGCCATTAAAGAGGAGATTGATGCTCACAAAGACGATTACCACCGGGTC AAGGCCTCTGGGGAAAAAGTGATCGAAGGTCAAACTGACCCAGATTACCAGCTTCTGGGCCAGCGACTGG AGGGTCTAGATACTGACTGGGATGCTCTGTGGCGGATGTGGGAGAGCCGAGGGAACACCCTCACCCAGTG CCTGGGCTTCCAGGAGTTCCAGAAAGATGCCAAGCAGGCTGAAGCCATCCTCAGCAACCAGGAATACACT CTGGCTCACCTGGAGCCCCCAGACTCCCTAGCAGCTGCAGAGGCCGGCATCCGAAAGTTTGAGGATTTCT TAGTGTCTATGGAAAACAACCGAGATAAAGTCCTGAGTCCTGTGGACTCCGGAAACATGCTGGTGTCTGA GGGCAACCTGTACTCGAACAAGATCAAAGAGAAGGTGCAGCTGATTGAAGACAGGCACATGAAGAACAAT GAGAAAGCCCAGGAGGTTACAGCTCTACTGAAAGACAACCTGGAGTTGCAGAACTTCCTCCAGAACTGCA AGGAGCTCACTCTCTGGATCAATGACAAGCTGCTGACGTCTCCAGACATCTCCTATGACGAAGCCCGCAA CCTTCACAACAAATGGATGAAACACCAGGCATTTATGGCAGAGCTGGCTTCACACCAAGGGTGGCTTGAG AACATCGTCGCGGAGGGAAGGCAGCTGATGGCAGAGAAGCCCCAGTTCACGGATGTGGTGTCAGAAAGGC TGGAAGCCCTGCACAAGCTCTGGGATGAGCTGCAGACCACCACAAAGGCGAAGACGGAGCAACTCTCTGC TGCCCGGAGCTCTGACCTGCGCTTGCAAACCCACGCCGACCTTAGCAAATGGATCAGCGCCATGGAGGAC CAGCTGCGATCCGACGACCTGGGCAAGGATCTGACCACTGTCAACCGGATGTTGGTTAAGTTGAAGCGAG TGGAGGAACAAGTGAATTTGCGGAAGGAGGAGCTGGAGGAACTCTTTGCAGAGGCACCCCCACTGGGAGC AGAGGCAGGAGACACGGACATGAGCATTGAAAAGCGGTTCCTAGACCTTCTAGAACCTCTGGGGAGGAGG AAGAAGCAGCTGGAATTATCCAAAGCCAAGCTACAGATCAGCCGGGACTTAGAGGATGAGACGCTCTGGG TGGAAGAGAGGCTGCCGTTGGCGCAGTCAGCTGACTATGGCACTAATCTGCAAACAGTGCAGCTGTTCAT GAAGAAGAACCAGACCCTGCAGAATGAGATCCTAGGCCACGCACCACGGGTGGAGGATGTGCTACATCGA GGGCAGGAGCTGGTGAAGGCAGCCGAGATCGACTGCCAAGACATCGAGGAGCGCTTGGGGCATCTGCAGA GCTCGTGGGACACGCTGCGGGAGGCGGCAGCTGGCCGGCTGCAGCGCCTGCGGGAAGCTCACGAGGCACA GCAGTACTACCTGGATGCGGGCGAGGCTGAAGCGTGGATCAGCGAGCAGGAGCTCTACGTGTTCTCTGAC GAACCCCCTAAGGACGAAGAGGGCGCCATCGTGATGCTCAAGCGGCACCTTCGGCAGCAGCGCACGGTGG AGGAATATGGAAGGAATATCAAACAGCTGGCTGGCCGCGCCCAGAGCCTGCTGTCTGCAGGCCACCCGGA GGGGGAGCAGATCATCAGACTTCAGGGGCAAGTGGATAAGCAGTATGCCGGGCTGAAGGACATGGCTGAG GAACGGAGGCGGAAGCTAGAGAACATGTACCACCTGTTCCAGCTGAAGAGAGAGGCTGACGACTTGGAGC AGTGGATTACGGAGAAAGAGATGGTGGCCTCATCCCAAGAAATGGGGCAGGACTTTGACCACGTGACTAT GCTCCGTGACAAGTTCCGAGACTTTGCCCGGGAGACCGGGGCTATCGGGCAGGAGCGAGTAGACAATGTG AATTCTATCATTGAGCGTCTCATCGATGCGGGCCACAGCGAGGCAGCCACCATTGCAGAGTGGAAGGATG GGCTGAATGACATGTGGGCAGATCTGCTGGAGCTCATTGACACCCGCATGCAGCTGCTGGCTGCCTCCTA CGACCTGCACCGCTATTTCTACACGGGCACCGAGATCCTGGGCCTCATAGATGAGAAGCACAGAGAGTTG CCAGAGGACGTGGGGCTGGACGCCAGCACAGCTGAGTCCTTCCACCGGGTCCACACTGCCTTCGAGCGGG AGCTCCACCTCCTGGGTGTGCAGGTGCAGCAGTTCCAGGACGTGGCCACCCGACTGCAGACAGCGTATGC TGGGGAGAAGGCAGATGCTATCCAGAGCAAGGAGCAGGAGGTGTCCGCGGCCTGGCAGGCCCTGCTGGAC GCGTGTGCTGGGCGCCGAGCTCAGCTGGTAGATACGGCAGACAAGTTCCGCTTCTTCAGCATGGTGCGTG ACCTCCTCTCCTGGATGGAGAGCATCATCCGGCAGATCGAGACCCAGGAGAGACCCAGGGATGTCTCCTC GGTGGAACTGCTCTTGAAGTATCACCAGGGCATCAAGGCAGAGATCAATACCAGGGCCAAGAACTTCAGC ACCTGCCTAGAACTTGGGGAGTCCCTGCTACAGAGGCAACACCAGGCTTCAGACGAGATCCGGGAGAAAT TACAGCAAGTGATATCCAGGAGGCAGGAGATGAATGACAAGTGGGAAGCCCGCTCTGACAGGCTCCACAT GCTGCTGGAGGTGTGCCAGTTCTCGAGGGACGCTTCTGTGGCCGAGGCATGGCTGATTGCCCAGGAGCCC TACCTGGCCAGCCGGGACTTTGGACATACGGTGGACAGCGTGGAAAAGCTCATCAAGAGGCATGAGGCTT TTGAGAAGTCCACAGCCAGCTGGGCAGAGCGCTTTGCTGCCCTGGAGAAGCCCACCACGCTTGAGCTTAA GGAACGCCAGACTCCAGACAGACCCACAGAGGAACCTGGGCCTCAAGAGGAGGAAGGCGAGACAGCTGGC GAGGCTCCCCAGGTCCACCATGCGGCCACGGAGAGAACATCCCCGGTCAGTTTTATGTCCCGCTTGTCTA GCTCCTGGGAGTCACTGCTGCCAGAGCCTGCTCACCCCTTCTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_212522
Insert Size	6414 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_212522.1, NP_997687.1
RefSeq Size	6810 bp
RefSeq ORF	6414 bp
Locus ID	314251

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