Lig1 (NM_001024268) Rat Untagged Clone

CAT#: RN200286

Lig1 (untagged ORF) - Rat ligase I, DNA, ATP-dependent (Lig1), transcript variant 1, (10 ug)



  "NM_001024268" in other vectors (3)

CNY 11,780.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms MGC105416
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN200286 representing NM_001024268
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGAGAAGTATTATGTCATTTTTCCAACCCACAACAACAGAAGGTAAAGCGAAGAAGCCAGAGAAGG
AGATACCCAGCAGCATCAGAGAGAAGGAACCCCCTCCAAAGGTGGCATTGAAGGAGAGGAATCGAGCAGT
GCCTGAGAGTGATTCTCCAGTGAAGAGGCCAGGAAGGAAGGTGGCCCAGGTTCTGAGCAGTGAAGGGGAG
GACGAAGATGAAGCCCCTGGCACCCCCCAAGTCCAAAAGCCTGTGTCAGACTCCAAACAGAGCTCTCCTC
CCAGCCCTGACTCCTGTCCTGAGAACAGCAGCCCCTCCATGGACATCTCCCCATCAGGATTCCCAAAGCG
CCGAACTGCACGGAAGCAGCTCCCAAAACGGACAATTCAAGACACTCTTGAGGAGCACAATGAAGACAAA
GACAAAGCAGCTAAGAAAAGGAAAAAAGAAGATACACAGACCCCAACAGAAAGCCTCACAGAGGCTGAAG
AAGTAAAACAGAAGGAAGAACAGGTGGAGGACCAGCCCACAGTACCCCCTGAGCCCACAGAGTCCCCTGA
GTCAGTAACCCTCACAAAGACAGAGAACATTCCAATGTGTAAGGCAGGTGTGAAACAGAAGCCTCAGGAA
GAAGAACAGAGCAAGCCTCCTGCCAGGGGTGCCAAGCCACTGAGCAGTTTCTTCACACCCCGAAAACCAG
CAGTGAAAACTGAAGTCAAACAAGAAGAGTCAGACACTCCAAGGAAGGAAGAGACCAAGGGAGCCCCGGA
TCCAACCAATTATAATCCTTCCAAGAGTAACTACCATCCCATTGAAGATGCCTGCTGGAAACATGGCCAA
AAGGTTCCTTTTCTTGCTGTGGCCCGGACATTTGAGAAGATTGAGGAGGTGTCTGCTCGGCTTAAGATGG
TGGAGACACTGAGCAACCTGCTGCGCTCCGTGGTGGCCCTGTCCCCAACAGACCTGCTCCCTGTTCTCTA
TCTCAGCCTCAACCGCCTTGGGCCGCCTCAGCAGGGTCTAGAACTGGGTGTTGGTGATGGTGTCCTCCTC
AAGGCAGTTGCCCAGGCCACAGGTCGTCAGCTGGAGTCCATCCGGGCTGAGGTAGCTGAGAAGGGGGATG
TGGGGCTAGTGGCGGAGAACAGCCGAAGCACTCAGAGGCTCATGCTGCCTCCACCTCCACTTACTGTTTC
TGGAGTCTTTACCAAATTCTGTGACATTGCCCGGCTCACTGGCAGTGCTTCCATGGCCAAGAAAATGGAC
ATTATCAAGGGCCTTTTTGTTGCCTGCCGCTACTCAGAAGCCCGGTTTATTGCCAGGTCCCTAAGTGGGC
GGCTGCGCCTTGGGCTGGCTGAGCAGTCAGTACTGGCTGCTCTTGCCCAGGCTGTGAGCCTCACACCCCC
TGGCCAAGAATTTCCCACAGCCGTGGTGGATGCTGGGAAGGGCAAGACAGCAGAGGCCAGAAAGATGTGG
TTGGAAGAACAAGGCATGATCTTGAAGCAAACCTTCTGTGAGGTGCCTGATCTGGATCGCATCATCCCTG
TGCTGCTGGAACATGGCTTGGAAAGTCTCCCAGAGCACTGCAAGCTGAGCCCAGGGGTTCCTCTTAAACC
AATGCTGGCCCATCCCACTCGGGGTGTCCGTGAGGTCCTGAAACGCTTTGAGGAGGTCGACTTCACCTGC
GAGTATAAATACGATGGGCAGCGGGCACAGATACATGTTCTGGAAGGTGGAGAAGTGAAGATCTTCAGCA
GGAACCAGGAAGACAACTCAGGAAAGTATCCTGACATCATCAGCCGCATCCCCAAGATTAAACACCCCTC
AGTCACATCTTTCATTCTGGACACTGAGGCTGTGGCCTGGGACCGGGAAAAGAAGCAGATCCAGCCATTC
CAAGTGCTCACCACACGCAAGCGCAAGGAGGTTGATGCATCGGAGATACAGGTACAAGTGTGTCTGTATG
CTTTTGACCTCATCTACCTCAACGGAGAGTCCCTGGCTCGCCAGCCCCTGTCTCGACGCCGGCAGCTACT
CCGTGAGAACTTTGTGGAGACAGAGGGTGAGTTTGTCTTTGCCACCTCCTTGGACACCAAGGACATCGAG
CAGATTGCCGAGTTCTTAGAGCAGTCAGTGAAGGACTCCTGTGAGGGCCTGATGGTGAAGACCCTGGATG
TTGATGCTACCTATGAGATCGCCAAGAGGTCACACAACTGGCTCAAGCTGAAGAAGGACTACCTTGAAGG
TGTGGGCGACACTCTAGACCTTGTGGTGATTGGTGCCTACCTGGGCCGGGGGAAGCGGGCTGGCCGGTAT
GGGGGCTTCCTGTTGGCTGCCTACGATGAGGAGAGTGAAGAGCTGCAGGCCATATGCAAGCTGGGAACCG
GATTCAGCGACGAGGAGCTGGAAGAACATCATCAGAGCATGCAGGCCCTTTTATTGCCTACCCCACGCCC
CTATGTGAGGATTGATGGGGCAGTGGCCCCAGACCACTGGCTAGACCCGAGTGTTGTGTGGGAGGTGAAA
TGTGCTGATCTCTCCCTGTCTCCCATCTACCCTGCTGCTCGGGGCCTGGTGGACAAAGAAAAAGGGATCT
CCCTTCGTTTCCCTCGGTTCATTCGTGTCCGTGAGGATAAGCAGCCAGAGCAGGCCACTACCAGTGACCA
GGTGGCCTCTTTGTACCGGAAGCAGAGTCAGATTCAGAACCAGCAAAGCTCAGACTTGGACTCTGATGTT
GAAGATTACTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001024268
Insert Size 2742 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001024268.2, NP_001019439.1
RefSeq Size 3069 bp
RefSeq ORF 2742 bp
Locus ID 81513
Gene Summary catalyzes the joining of blunt ended DNA [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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