PITRM1 (NM_001242307) Human Tagged ORF Clone
CAT#: RG235134
- TrueORF®
PITRM1 (tGFP-tagged) - Homo sapiens pitrilysin metallopeptidase 1 (PITRM1), transcript variant 1
ORF Plasmid: DDK
"NM_001242307" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 11,400.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | MP1; PreP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG235134 representing NM_001242307
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCGCTGCGGCGGGCGGCAGGGCCTGTGTGTGCTGAGGCGGCTGAGCGGCGGACATGCACACCACA GAGCGTGGCGATGGAACAGTAACCGGGCTTGTGAGAGGGCTCTGCAGTATAAACTAGGAGACAAGATCCA TGGATTCACCGTAAACCAGGTGACATCTGTTCCCGAGCTGTTCCTGACTGCAGTGAAGCTCACCCATGAT GACACAGGAGCCAGGTATTTACACCTGGCCAGAGAAGACACGAATAATCTGTTCAGCGTGCAGTTCCGTA CTACTCCCATGGACAGTACTGGTGTTCCTCACATTCTTGAGCATACCGTCCTTTGTGGGTCTCAGAAATA TCCGTGCAGAGACCCTTTCTTCAAAATGTTGAACCGGTCCCTCTCCACGTTCATGAACGCCTTCACAGCT AGTGATTATACTCTGTATCCATTTTCCACACAAAATCCCAAGGACTTTCAGAATCTCCTCTCGGTGTATT TGGATGCCACCTTTTTCCCATGTTTACGCGAGCTGGATTTCTGGCAGGAAGGATGGCGGCTGGAACATGA GAATCCGAGCGACCCCCAGACGCCCTTGGTCTTTAAAGGAGTCGTCTTTAATGAGATGAAGGGAGCGTTT ACAGACAATGAGAGGATATTCTCCCAGCACCTTCAGAACAGACTTCTTCCTGACCACACGTACTCAGTGG TCTCCGGGGGTGACCCACTGTGCATCCCGGAGCTTACATGGGAGCAGCTTAAGCAGTTTCATGCCACTCA CTATCACCCAAGCAATGCTAGGTTCTTCACGTACGGTAATTTTCCATTAGAACAGCATCTGAAACAAATT CACGAGGAAGCACTGAGCAAATTCCAGAAAATTGAACCAAGCACCGTGGTGCCAGCTCAGACACCCTGGG ACAAGCCTAGGGAATTCCAGATAACATGTGGCCCGGATTCATTTGCTACAGATCCCTCTAAACAAACAAC CATCAGCGTTAGCTTCCTCTTACCGGACATCACCGACACATTTGAAGCCTTCACATTAAGTCTTCTGTCT TCACTCTTGACTTCTGGGCCCAATTCTCCCTTTTACAAAGCCTTGATTGAATCTGGCCTTGGCACAGACT TTTCTCCTGATGTTGGATATAATGGCTACACGAGGGAGGCCTACTTTAGTGTCGGCCTCCAAGGGATTGC GGAGAAAGACATTGAGACCGTCAGAAGCCTCATAGACAGAACGATTGATGAAGTAGTTGAGAAAGGATTT GAAGATGATCGAATTGAGGCTTTACTTCATAAAATTGAAATACAGATGAAACATCAGTCTACCAGCTTTG GGCTGATGCTGACATCATACATAGCTTCTTGCTGGAACCATGATGGGGACCCTGTGGAGCTCTTGAAGTT GGGAAATCAGTTAGCTAAATTCAGACAGTGCCTGCAGGAAAATCCAAAATTTTTGCAAGAAAAAGTAAAA CAGTATTTTAAGAATAACCAGCATAAGCTGACTTTATCGATGAGGCCAGATGACAAGTATCACGAGAAGC AGGCACAGGTGGAAGCCACGAAGCTCAAGCAGAAGGTCGAGGCTCTGTCCCCCGGAGACAGGCAGCAGAT CTACGAGAAAGGTCTAGAATTACGGAGTCAACAAAGCAAACCTCAAGATGCCTCTTGTCTGCCAGCGTTG AAAGTTTCCGATATTGAACCCACCATACCTGTCACAGAGTTGGACGTGGTCCTGACAGCTGGAGATATCC CTGTTCAGTACTGCGCCCAGCCCACCAATGGCATGGTGTATTTCCGGGCCTTCTCCAGCCTGAACACACT CCCCGAGGAGCTGAGGCCCTATGTGCCCCTCTTCTGCAGCGTCCTCACCAAGCTGGGCTGCGGCCTTCTT GACTACCGGGAGCAGGCTCAGCAGATAGAATTGAAGACCGGAGGGATGAGTGCTTCTCCCCACGTGCTCC CCGACGACTCACACATGGACACCTACGAGCAGGTAGGTGTGCTTTTCTCCTCTCTCTGCCTGGATCGAAA CCTGCCAGACATGATGCAGCTATGGAGTGAAATATTTAACAACCCGTGCTTTGAAGAAGAGGAGCACTTC AAGGTGCTGGTGAAGATGACCGCCCAGGAGCTCGCCAATGGAATTCCTGACTCTGGGCACCTGTACGCAT CCATCAGGGCAGGCCGGACCCTCACGCCCGCAGGGGACCTGCAGGAGACCTTCAGCGGGATGGATCAGGT GCGGCTGATGAAGAGGATTGCAGAAATGACAGATATCAAACCCATCCTGAGGAAGCTCCCGCGTATCAAG AAACACTTGTTAAATGGTGATAATATGAGGTGTTCAGTGAATGCGACTCCTCAGCAGATGCCTCAGACAG AAAAAGCGGTCGAAGACTTCCTTAGAAGCATCGGTCGGAGTAAAAAGGAACGGAGGCCTGTGCGCCCACA CACGGTCGAGAAACCTGTGCCCAGCAGCTCTGGTGGAGATGCCCACGTTCCCCATGGCTCCCAGGTCATT AGGAAGCTGGTCATGGAACCCACCTTCAAGCCCTGGCAGATGAAGACTCACTTCCTGATGCCCTTCCCGG TGAATTACGTGGGTGAATGCATCCGAACTGTCCCCTACACGGACCCAGATCATGCCAGTCTTAAAATCCT TGCACGTTTGATGACTGCCAAATTCTTGCATACAGAAATTCGAGAAAAAGGCGGTGCTTATGGTGGAGGC GCAAAACTCAGCCACAATGGGATTTTCACCCTTTACTCTTACAGGGACCCAAATACAATAGAGACGCTCC AGTCTTTTGGGAAGGCTGTCGACTGGGCTAAGTCTGGAAAATTCACACAGCAAGACATCGACGAAGCCAA ACTTTCTGTCTTCTCAACCGTAGATGCTCCTGTCGCTCCTTCAGACAAAGGAATGGACCACTTCTTGTAC GGCCTCTCGGATGAGATGAAGCAGGCCCACAGAGAGCAGCTCTTTGCTGTCAGCCACGACAAGCTCCTGG CCGTGAGCGATAGATACCTCGGCACTGGGAAGAGCACACACGGCCTGGCCATCCTCGGACCCGAGAACCC GAAAATTGCCAAGGACCCATCCTGGATCATCCAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235134 representing NM_001242307
Red=Cloning site Green=Tags(s) MWRCGGRQGLCVLRRLSGGHAHHRAWRWNSNRACERALQYKLGDKIHGFTVNQVTSVPELFLTAVKLTHD DTGARYLHLAREDTNNLFSVQFRTTPMDSTGVPHILEHTVLCGSQKYPCRDPFFKMLNRSLSTFMNAFTA SDYTLYPFSTQNPKDFQNLLSVYLDATFFPCLRELDFWQEGWRLEHENPSDPQTPLVFKGVVFNEMKGAF TDNERIFSQHLQNRLLPDHTYSVVSGGDPLCIPELTWEQLKQFHATHYHPSNARFFTYGNFPLEQHLKQI HEEALSKFQKIEPSTVVPAQTPWDKPREFQITCGPDSFATDPSKQTTISVSFLLPDITDTFEAFTLSLLS SLLTSGPNSPFYKALIESGLGTDFSPDVGYNGYTREAYFSVGLQGIAEKDIETVRSLIDRTIDEVVEKGF EDDRIEALLHKIEIQMKHQSTSFGLMLTSYIASCWNHDGDPVELLKLGNQLAKFRQCLQENPKFLQEKVK QYFKNNQHKLTLSMRPDDKYHEKQAQVEATKLKQKVEALSPGDRQQIYEKGLELRSQQSKPQDASCLPAL KVSDIEPTIPVTELDVVLTAGDIPVQYCAQPTNGMVYFRAFSSLNTLPEELRPYVPLFCSVLTKLGCGLL DYREQAQQIELKTGGMSASPHVLPDDSHMDTYEQVGVLFSSLCLDRNLPDMMQLWSEIFNNPCFEEEEHF KVLVKMTAQELANGIPDSGHLYASIRAGRTLTPAGDLQETFSGMDQVRLMKRIAEMTDIKPILRKLPRIK KHLLNGDNMRCSVNATPQQMPQTEKAVEDFLRSIGRSKKERRPVRPHTVEKPVPSSSGGDAHVPHGSQVI RKLVMEPTFKPWQMKTHFLMPFPVNYVGECIRTVPYTDPDHASLKILARLMTAKFLHTEIREKGGAYGGG AKLSHNGIFTLYSYRDPNTIETLQSFGKAVDWAKSGKFTQQDIDEAKLSVFSTVDAPVAPSDKGMDHFLY GLSDEMKQAHREQLFAVSHDKLLAVSDRYLGTGKSTHGLAILGPENPKIAKDPSWIIQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001242307 |
ORF Size | 3114 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001242307.1, NP_001229236.1 |
RefSeq Size | 3508 bp |
RefSeq ORF | 3117 bp |
Locus ID | 10531 |
UniProt ID | Q5JRX3 |
Protein Families | Druggable Genome, Protease |
Gene Summary | The protein encoded by this gene is an ATP-dependent metalloprotease that degrades post-cleavage mitochondrial transit peptides. The encoded protein binds zinc and can also degrade amyloid beta A4 protein, suggesting a possible role in Alzheimer's disease. [provided by RefSeq, Dec 2016] |
Documents
Product Manuals |
FAQs |
SDS |