Calpain 2 (CAPN2) (NM_001146068) Human Tagged ORF Clone
CAT#: RG228507
- TrueORF®
CAPN2 (tGFP-tagged) - Human calpain 2, (m/II) large subunit (CAPN2), transcript variant 2
ORF Plasmid: DDK
"NM_001146068" in other vectors (4)
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CNY 5,990.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CANP2; CANPL2; CANPml; mCANP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG228507 representing NM_001146068
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGATCTGCGCTGACCCCCAGTTTATCATTGGAGGAGCCACCCGCACAGACATCTGCCAAGGAGCCC TGGGTGACTGCTGGCTGCTGGCAGCCATTGCCTCCCTCACCTTGAATGAAGAAATCCTGGCTCGAGTCGT CCCCCTAAACCAGAGCTTCCAGGAAAACTATGCAGGGATCTTTCACTTCCAGTTCTGGCAATACGGCGAG TGGGTGGAGGTGGTGGTGGATGACAGGCTGCCCACCAAGGACGGGGAGCTGCTCTTTGTGCATTCAGCCG AAGGGAGCGAGTTCTGGAGCGCCCTGCTGGAGAAGGCATACGCCAAGATCAACGGATGCTATGAAGCGCT ATCAGGGGGTGCCACCACTGAGGGCTTCGAAGACTTCACCGGAGGCATTGCTGAGTGGTATGAGTTGAAG AAGCCCCCTCCCAACCTGTTCAAGATCATCCAGAAAGCTCTGCAAAAAGGCTCTCTCCTTGGCTGCTCCA TCGACATCACCAGCGCCGCGGACTCGGAGGCCATCACGTTTCAGAAGCTGGTGAAGGGGCACGCGTACTC GGTCACCGGAGCCGAGGAGGTTGAAAGTAACGGAAGCCTACAGAAACTGATCCGCATCCGAAATCCCTGG GGAGAAGTGGAGTGGACAGGGCGGTGGAATGACAACTGCCCAAGCTGGAACACTATAGACCCAGAGGAGA GGGAAAGGCTGACCAGACGGCATGAAGATGGAGAATTCTGGATGTCTTTCAGTGACTTCCTGAGGCACTA TTCCCGCCTGGAGATCTGTAACCTGACCCCAGACACTCTCACCAGCGATACCTACAAGAAGTGGAAACTC ACCAAAATGGATGGGAACTGGAGGCGGGGCTCCACCGCGGGAGGTTGCAGGAACTACCCGAACACATTCT GGATGAACCCTCAGTACCTGATCAAGCTGGAGGAGGAGGATGAGGACGAGGAGGATGGGGAGAGCGGCTG CACCTTCCTGGTGGGGCTCATTCAGAAGCACCGACGGCGGCAGAGGAAGATGGGCGAGGACATGCACACC ATCGGCTTTGGCATCTATGAGGTTCCAGAGGAGTTAAGTGGGCAGACCAACATCCACCTCAGCAAAAACT TCTTCCTGACGAATCGCGCCAGGGAGCGCTCAGACACCTTCATCAACCTCCGGGAGGTGCTCAACCGCTT CAAGCTGCCGCCAGGAGAGTACATTCTCGTGCCTTCCACCTTCGAACCCAACAAGGATGGGGATTTCTGC ATCCGGGTCTTTTCTGAAAAGAAAGCTGACTACCAAGCTGTCGATGATGAAATCGAGGCCAATCTTGAAG AGTTCGACATCAGCGAGGATGACATTGATGATGGATTCAGGAGACTGTTTGCCCAGTTGGCAGGAGAGGA TGCGGAGATCTCTGCCTTTGAGCTGCAGACCATCCTGAGAAGGGTTCTAGCAAAGCGCCAAGATATCAAG TCAGATGGCTTCAGCATCGAGACATGCAAAATTATGGTTGACATGCTAGATTCGGACGGGAGTGGCAAGC TGGGGCTGAAGGAGTTCTACATTCTCTGGACGAAGATTCAAAAATACCAAAAAATTTACCGAGAAATCGA CGTTGACAGGTCTGGTACCATGAATTCCTATGAAATGCGGAAGGCATTAGAAGAAGCAGGTTTCAAGATG CCCTGTCAACTCCACCAAGTCATCGTTGCTCGGTTTGCAGATGACCAGCTCATCATCGATTTTGATAATT TTGTTCGGTGTTTGGTTCGGCTGGAAACGCTATTCAAGATATTTAAGCAGCTGGATCCCGAGAATACTGG AACAATAGAGCTCGACCTTATCTCTTGGCTCTGTTTCTCAGTACTT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG228507 representing NM_001146068
Red=Cloning site Green=Tags(s) MEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLNQSFQENYAGIFHFQFWQYGE WVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELK KPPPNLFKIIQKALQKGSLLGCSIDITSAADSEAITFQKLVKGHAYSVTGAEEVESNGSLQKLIRIRNPW GEVEWTGRWNDNCPSWNTIDPEERERLTRRHEDGEFWMSFSDFLRHYSRLEICNLTPDTLTSDTYKKWKL TKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDEEDGESGCTFLVGLIQKHRRRQRKMGEDMHT IGFGIYEVPEELSGQTNIHLSKNFFLTNRARERSDTFINLREVLNRFKLPPGEYILVPSTFEPNKDGDFC IRVFSEKKADYQAVDDEIEANLEEFDISEDDIDDGFRRLFAQLAGEDAEISAFELQTILRRVLAKRQDIK SDGFSIETCKIMVDMLDSDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKM PCQLHQVIVARFADDQLIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIELDLISWLCFSVL SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001146068 |
ORF Size | 1866 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001146068.1, NP_001139540.1 |
RefSeq Size | 3270 bp |
RefSeq ORF | 1869 bp |
Locus ID | 824 |
UniProt ID | P17655 |
Protein Families | Druggable Genome, Protease |
Protein Pathways | Alzheimer's disease, Apoptosis, Focal adhesion |
Gene Summary | The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC228507 | CAPN2 (Myc-DDK-tagged)-Human calpain 2, (m/II) large subunit (CAPN2), transcript variant 2 |
CNY 4,632.00 |
|
RC228507L3 | Lenti-ORF clone of CAPN2 (Myc-DDK-tagged)-Human calpain 2, (m/II) large subunit (CAPN2), transcript variant 2 |
CNY 7,320.00 |
|
RC228507L4 | Lenti-ORF clone of CAPN2 (mGFP-tagged)-Human calpain 2, (m/II) large subunit (CAPN2), transcript variant 2 |
CNY 7,320.00 |
|
SC327142 | CAPN2 (untagged)-Human calpain 2 (m/II) large subunit (CAPN2) transcript variant 2 |
CNY 9,980.00 |
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