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CCDC108 (CFAP65) (NM_194302) Human Tagged ORF Clone
CAT＃: RG218466
- TrueORF®
CCDC108 (tGFP-tagged) - Human coiled-coil domain containing 108 (CCDC108), transcript variant 1
ORF Plasmid: DDK
"NM_194302" in other vectors (2)
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CNY 20,900.00
货期*
4周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CCDC108; SPGF40 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218466 representing NM_194302
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTACCTTAACCGGTTGTAGACTGGTGGAGAAAACCCAGAAGGTAGAGAATCCATCAGTCTCATTTG CCTCTTCTTTCCCCCTTATTCCTCTTCTCCTAAGAGGCAAGAGTGTTCAGAAGAAACAAGCAGAGAGTAA AAGCCAGATAAAGCTCCATACTCAGAGTGCTCCCTTTGGACTGTGTCCCAAGGACATGATGCTCACCCAG GCTCCAAGCTCCGTCGTGAGGTCCAGGAACAGCAGGAACCACACCGTGAACTCTGGTGGATCCTGCCTGA GTGCCAGCACAGTGGCCATCCCTGCCATCAACGACAGCAGTGCAGCCATGAGTGCCTGCAGCACCATCAG CGCCCAGCCCGCAAGCTCCATGGACACTCAGATGCACTCCCCAAAGAAGCAGGAGAGAGTGAACAAGAGG GTCATCTGGGGCATTGAGGTGGCTGAGGAGCTGCATTGGAAAGGCTGGGAGCTAGGAAAGGAGACCACAA GGAATCTGGTTCTGAAAAATCGATCCTTGAAACTCCAGAAGATGAAGTACAGGCCCCCCAAGACCAAGTT CTTCTTCACGGTCATCCCTCAGCCCATCTTCCTGAGCCCAGGCATAACCCTCACGCTCCCCATCGTCTTC CGGCCTCTGGAGGCGAAGGAGTACATGGACCAGCTGTGGTTTGAGAAAGCGGAGGGGATGTTCTGTGTCG GCCTACGGGCCACCCTGCCCTGCCACAGGCTGATCTGCCGCCCACCATCCCTGCAGCTGCCCATGTGTGC TGTGGGAGATACGACTGAGGCCTTTTTCTGCCTGGATAATGTGGGGGACCTGCCCACCTTCTTCACCTGG GAGTTCTCCAGCCCATTCCAGATGCTGCCCGCCACGGGGCTCCTGGAGCCAGGCCAGGCCTCTCAGATCA AGGTGACCTTTCAGCCCCTTACAGCCGTCATCTACGAGGTGCAGGCCACGTGCTGGTACGGGGCGGGCAG CCGGCAGAGGAGCAGCATCCAGCTGCAGGCTGTGGCCAAGTGCGCCCAGCTGCTGGTGAGCATAAAGCAC AAGTGCCCGGAGGACCAGGATGCCGAGGGCTTCCAGAAGCTGTTGTACTTTGGCTCTGTTGCTGTGGGCT GCACCTCGGAGAGGCAGATCAGGCTACACAACCCGTCGGCGGTAAATGCCCCCTTCAGGATTGAAATTTC CCCGGATGAACTGGCCGAAGACCAGGCCTTCTCATGCCCCACGGCCCATGGCATCGTGCTTCCGGGAGAG AAGAAATGTGTGTCGGTGTTCTTCCACCCCAAGACTCTGGACACCAGAACTGTGGACTACTGCTCCATCA TGCCTTCTGGCTGTGCCTCCAAGACCCTGCTTAAAGTCGTTGGTTTCTGTAGAGGCCCTGCTGTGTCCCT GCAGCACTACTGTGTCAACTTCAGCTGGGTCAACCTTGGGGAGCGCTCCGAGCAGCCCCTGTGGATTGAG AACCAATCGGACTGCACGGCCCACTTCCAGTTTGCCATCGACTGCTTGGAGAGTGTCTTTACCATCAGGC CTGCCTTTGGGACGCTGGTGGGCAAGGCCCGTATGACCCTGCACTGTGCCTTCCAGCCCACTCACCCCAT CATCTGCTTTCGGCGTGTGGCCTGTCTCATCCACCACCAGGACCCACTGTTCCTGGACCTGATGGGGACC TGCCACTCGGACAGCACCAAGCCAGCCATCCTGAAGCCTCAGCACCTCACCTGGTACCGCACACACCTGG CCCGGGGCCTGACGCTCTACCCCCCTGACATCCTGGATGCCATGCTGAAGGAGAAGAAGCTGGCACAGGA CCAGAACGGGGCTCTCATGATTCCCATCCAGGATCTGGAGGACATGCCGGCCCCGCAGTACCCTTATATC CCCCCCATGACCGAGTTCTTCTTCGACGGCACCAGCGACATAACCATCTTCCCCCCGCCCATCAGTGTAG AGCCTGTCGAGGTAGACTTCGGTGCCTGCCCAGGGCCTGAGGCCCCCAACCCTGTACCCCTGTGCCTGAT GAACCACACCAAGGGCAAGATCATGGTGGTCTGGACGCGAAGGTCTGACTGCCCCTTCTGGGTGACTCCA GAGAGCTGCGACGTGCCCCCACTCAAGTCCATGGCCATGCGCCTGCACTTCCAGCCGCCTCACCCCAACT GCCTTTACACGGTGGAGCTCGAAGCCTTCGCCATCTATAAGGTCCTGCAGAGCTACAGTAATATTGAGGA GGACTGCACCATGTGCCCATCCTGGTGCCTGACGGTGCGGGCACGAGGCCACAGCTATTTCGCTGGCTTT GAGCACCACATCCCCCAGTATTCCCTAGATGTCCCCAAGCTATTTCCAGCAGTGTCCTCCGGTGAGCCCA CCTACCGCAGCCTGCTCCTGGTCAACAAAGACTGCAAGCTGCTGACCTTCAGCCTGGCCCCCCAGAGAGG CTCAGACGTCATCCTTCGGCCCACTTCGGGCCTTGTGGCACCCGGGGCCCACCAGATCATCCTCATCTGC ACCTACCCTGAGGGCAGCTCCTGGAAGCAGCACACTTTCTATCTGCAGTGCAATGCTTCCCCCCAGTATC TCAAGGAGGTGAGCATGTACAGCCGGGAGGAGCCACTGCAGCTGAAGCTGGACACCCACAAAAGCCTCTA CTTCAAGCCCACCTGGGTGGGCTGCTCCTCCACCAGCCCCTTCACCTTCCGCAACCCCTCGCGTCTGCCC CTGCAGTTCGAGTGGAGGGTCTCTGAGCAGCATCGAAAGCTGCTGGCTGTCCAGCCCTCCAGGGGGCTAA TCCAGCCCAACGAGAGACTTACGCTGACGTGGACCTTCAGCCCTTTGGAGGAGACCAAGTACCTGTTCCA AGTGGGGATGTGGGTCTGGGAAGCCGGCCTGTCCCCAAATGCCAACCCCGCTGCCACCACCCACTACATG CTCCGGCTGGTGGGCGTTGGGCTCACCAGCAGCCTCTCTGCAAAGGAAAAGGAGCTGGCCTTTGGGAATG TGCTGGTGAACAGCAAGCAGTCCAGGTTCCTTGTCCTCCTGAATGACGGCAACTGCACCCTCTATTACCG CCTCTACCTGGAGCAGGGCAGCCCTGAGGCCGTTGACAACCACCCCCTCGCTCTGCAGCTGGACCGAACA GAGGGGAGCATGCCACCCCGGTCCCAGGACACCATCTGCCTGACTGCCTGTCCCAAGCAGCGGTCCCAGT ACTCCTGGACCATCACCTACTCTCTCCTTTCCCACAGAGATAACAAGGCTGGGGAGAAGCAGGAGCTGTG CTGCGTCTCCCTGGTGGCCGTGTACCCCTTGCTTTCCATCCTGGATGTCAGCTCCATGGGCAGTGCTGAG GGTATCACCCGGAAGCACCTGTGGCGCCTCTTCTCTCTGGACCTGCTTAACAGTTACTTGGAGCGTGACC CCACCCCCTGTGAGCTCACCTACAAGGTGCCCACCCGGCACAGCATGAGCCAGATCCCCCCCGTCCTCAC CCCTTTAAGGCTTGACTTCAATTTCGGGGCCGCACCATTCAAGGCCCCACCTTCCGTGGTATTCCTGGCC CTGAAGAACAGCGGAGTGGTGTCCCTGGACTGGGCCTTCCTCCTTCCAAGTGACCAGCGGATTGACGTGG AGCTCTGGGCAGAGCAAGCAGAGTTGAATTCCACTGAGCTCCACCAGATGCGCGTGCAGGACAATTGCCT CTTCTCCATCAGCCCCAAGGCTGGGAGCCTGAGTCCTGGGCAGGAGCAGATGGTGGAGTTAAAATACAGC CACCTGTTCATCGGTACTGATCACCTCCCAGTGCTCTTCAAGGTGTCCCATGGCCGGGAGATCCTGCTAA ATTTCATAGGTGTGACAGTGAAGCCGGAGCAGAAGTATGTGCACTTCACCTCTACTACCCACCAGTTCAT CCCCATTCCCATTGGTGACACGCTACCCCCACGGCAGATTTATGAGCTGTATAATGGTGGCTCAGTGCCC GTGACATATGAGGTCCAGACCGATGTCCTGTCACAGGTTCAGGAAAAAAATTTTGATCACCCCATCTTTT GCTGCCTCAACCCCAAAGGGGAGATCCAGCCAGGCAGCACTGCCCGGGTCTTGTGGATCTTCTCACCTAT CGAGGCCAAGACCTACACGGTGGACGTGCCCATACACATCCTGGGATGGAACTCGGCCCTCATCCACTTC CAGGGAGTGGGCTACAACCCCCATATGATGGGGGACACAGCCCCATTCCACAACATCTCCTCGTGGGACA ACAGTTCCATACACTCTAGGCTGGTGGTGCCTGGACAGAATGTCTTCCTGTCCCAGTCTCATATTTCCCT GGGAAACATACCTGTGCAGAGCAAGTGCAGCCGCCTGCTCTTCCTCAACAACATCTCCAAGAACGAGGAA ATTGCCTTCTCCTGGCAGCCAAGTCCTCTAGATTTTGGGGAGGTGTCTGTGAGTCCCATGATAGGGGTGG TGGCTCCTGAAGAGACGGTCCCATTTGTGGTGACCTTGAGGGCCTCTGTGCATGCCAGCTTCTACAGTGC AGACCTGGTATGCAAGCTGTACTCGCAGCAGCTCATGAGGCAGTATCACAAGGAGCTGCAGGAGTGGAAG GACGAGAAGGTGCGGCAGGAAGTGGAGTTCACCATCACCGACATGAAAGTGAAGAAGAGAACATGCTGCA CAGCCTGTGAACCTGCGAGGAAGTACAAGACACTGCCTCCCATCAAGAACCAGCAGTCTGTCAGCCGGCC TGCCAGCTGGAAACTGCAGACCCCAAAGGAGGAGGTGTCCTGGCCCTGCCCCCAGCCACCCTCGCCAGGC ATGCTCTGCCTGGGCCTTACTGCCCGAGCCCATGCCACCGACTACTTTCTGGCTAACTTCTTCTCAGAGT TTCCCTGCCACTTTTTGCACCGGGAGCTGCCAAAGAGGAAGGCCCCCAGGGAAGAGTCAGAGACTTCTGA GGAAAAATCCCCTAACAAGTGGGGCCCTGTTTCCAAGCAGAAGAAGCAGCTCCTGGTTGACATTCTCACC ACAATAATCAGGGGCCTGCTGGAAGACAAGAACTTCCATGAGGCTGTGGACCAAAGCCTGGTGGAGCAGG TGCCGTACTTCCGCCAATTCTGGAATGAGCAGTCAACTAAGTTCATGGACCAGAAAAACAGCCTGTACTT AATGCCAATCCTGCCTGTACCCTCCAGCAGCTGGGAGGATGGGAAGGGCAAGCAGCCGAAGGAAGACAGA CCAGAGCACTATCCAGGGTTGGGAAAGAAGGAAGAGGGGGAGGAGGAGAAGGGTGAAGAGGAAGAAGAAG AGTTGGAGGAGGAAGAGGAGGAAGAAGAGGAGACAGAAGAGGAGGAGTTGGGCAAGGAGGAGATAGAGGA GAAGGAGGAGGAGAGGGATGAGAAGGAAGAGAAAGTGAGCTGGGCGGGCATCGGGCCCACACCACAGCCT GAGTCCCAGGAGTCCATGCAATGGCAGTGGCAACAGCAGCTGAATGTCATGGTGAAGGAGGAGCAAGAAC AGGACGAGAAGGAGGCCATCAGAAGGCTCCCGGCCTTCGCCAACCTGCAGGAGGCGCTGCTGGAGAACAT GATCCAGAACATCCTGGTGGAGGCGAGCCGCGGGGAGGTGGTACTCACCTCGCGGCCACGCGTCATCGCC CTGCCGCCGTTCTGCGTGCCCAGGAGTCTGACCCCGGACACGCTGCTGCCGACGCAGCAAGCAGAGGTAC TCCACCCGGTGGTGCCACTTCCTACCGACCTTCCG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218466 representing NM_194302
Red=Cloning site Green=Tags(s) MFTLTGCRLVEKTQKVENPSVSFASSFPLIPLLLRGKSVQKKQAESKSQIKLHTQSAPFGLCPKDMMLTQ APSSVVRSRNSRNHTVNSGGSCLSASTVAIPAINDSSAAMSACSTISAQPASSMDTQMHSPKKQERVNKR VIWGIEVAEELHWKGWELGKETTRNLVLKNRSLKLQKMKYRPPKTKFFFTVIPQPIFLSPGITLTLPIVF RPLEAKEYMDQLWFEKAEGMFCVGLRATLPCHRLICRPPSLQLPMCAVGDTTEAFFCLDNVGDLPTFFTW EFSSPFQMLPATGLLEPGQASQIKVTFQPLTAVIYEVQATCWYGAGSRQRSSIQLQAVAKCAQLLVSIKH KCPEDQDAEGFQKLLYFGSVAVGCTSERQIRLHNPSAVNAPFRIEISPDELAEDQAFSCPTAHGIVLPGE KKCVSVFFHPKTLDTRTVDYCSIMPSGCASKTLLKVVGFCRGPAVSLQHYCVNFSWVNLGERSEQPLWIE NQSDCTAHFQFAIDCLESVFTIRPAFGTLVGKARMTLHCAFQPTHPIICFRRVACLIHHQDPLFLDLMGT CHSDSTKPAILKPQHLTWYRTHLARGLTLYPPDILDAMLKEKKLAQDQNGALMIPIQDLEDMPAPQYPYI PPMTEFFFDGTSDITIFPPPISVEPVEVDFGACPGPEAPNPVPLCLMNHTKGKIMVVWTRRSDCPFWVTP ESCDVPPLKSMAMRLHFQPPHPNCLYTVELEAFAIYKVLQSYSNIEEDCTMCPSWCLTVRARGHSYFAGF EHHIPQYSLDVPKLFPAVSSGEPTYRSLLLVNKDCKLLTFSLAPQRGSDVILRPTSGLVAPGAHQIILIC TYPEGSSWKQHTFYLQCNASPQYLKEVSMYSREEPLQLKLDTHKSLYFKPTWVGCSSTSPFTFRNPSRLP LQFEWRVSEQHRKLLAVQPSRGLIQPNERLTLTWTFSPLEETKYLFQVGMWVWEAGLSPNANPAATTHYM LRLVGVGLTSSLSAKEKELAFGNVLVNSKQSRFLVLLNDGNCTLYYRLYLEQGSPEAVDNHPLALQLDRT EGSMPPRSQDTICLTACPKQRSQYSWTITYSLLSHRDNKAGEKQELCCVSLVAVYPLLSILDVSSMGSAE GITRKHLWRLFSLDLLNSYLERDPTPCELTYKVPTRHSMSQIPPVLTPLRLDFNFGAAPFKAPPSVVFLA LKNSGVVSLDWAFLLPSDQRIDVELWAEQAELNSTELHQMRVQDNCLFSISPKAGSLSPGQEQMVELKYS HLFIGTDHLPVLFKVSHGREILLNFIGVTVKPEQKYVHFTSTTHQFIPIPIGDTLPPRQIYELYNGGSVP VTYEVQTDVLSQVQEKNFDHPIFCCLNPKGEIQPGSTARVLWIFSPIEAKTYTVDVPIHILGWNSALIHF QGVGYNPHMMGDTAPFHNISSWDNSSIHSRLVVPGQNVFLSQSHISLGNIPVQSKCSRLLFLNNISKNEE IAFSWQPSPLDFGEVSVSPMIGVVAPEETVPFVVTLRASVHASFYSADLVCKLYSQQLMRQYHKELQEWK DEKVRQEVEFTITDMKVKKRTCCTACEPARKYKTLPPIKNQQSVSRPASWKLQTPKEEVSWPCPQPPSPG MLCLGLTARAHATDYFLANFFSEFPCHFLHRELPKRKAPREESETSEEKSPNKWGPVSKQKKQLLVDILT TIIRGLLEDKNFHEAVDQSLVEQVPYFRQFWNEQSTKFMDQKNSLYLMPILPVPSSSWEDGKGKQPKEDR PEHYPGLGKKEEGEEEKGEEEEEELEEEEEEEEETEEEELGKEEIEEKEEERDEKEEKVSWAGIGPTPQP ESQESMQWQWQQQLNVMVKEEQEQDEKEAIRRLPAFANLQEALLENMIQNILVEASRGEVVLTSRPRVIA LPPFCVPRSLTPDTLLPTQQAEVLHPVVPLPTDLP SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_194302 |
| ORF Size | 5775 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_194302.2, NP_919278.2 |
| RefSeq Size | 5975 bp |
| RefSeq ORF | 5778 bp |
| Locus ID | 255101 |
| UniProt ID | Q6ZU64 |
| Protein Families | Transmembrane |
| Gene Summary | The protein encoded by this gene has putative coiled-coil domains and may be a transmembrane protein. The chicken ortholog of this gene is involved in the Rose-comb mutation, which is a large chromosome inversion, resulting in altered comb morphology and defects in sperm motility. [provided by RefSeq, Aug 2016] |
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