RGS18 (NM_130782) Human Tagged ORF Clone
CAT#: RG204634
- TrueORF®
RGS18 (tGFP-tagged) - Human regulator of G-protein signaling 18 (RGS18)
ORF Plasmid: DDK
"NM_130782" in other vectors (5)
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CNY 4,000.00
CNY 4,370.00
CNY 1,000.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | RGS13 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG204634 representing NM_130782
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAACAACATTGCTTTTCTTTTCTCAAATAAATATGTGTGAATCAAAAGAAAAAACTTTTTTCAAGT TAATACATGGTTCAGGAAAAGAAGAAACAAGCAAAGAAGCCAAAATCAGAGCTAAGGAAAAAAGAAATAG ACTAAGTCTTCTTGTGCAGAAACCTGAGTTTCATGAAGACACCCGCTCCAGTAGATCTGGGCACTTGGCC AAAGAAACAAGAGTCTCCCCTGAAGAGGCAGTGAAATGGGGTGAATCATTTGACAAACTGCTTTCCCATA GAGATGGACTAGAGGCTTTTACCAGATTTCTTAAAACTGAATTCAGTGAAGAAAATATTGAATTTTGGAT AGCCTGTGAAGATTTCAAGAAAAGCAAGGGACCTCAACAAATTCACCTTAAAGCAAAAGCAATATATGAG AAATTTATACAGACTGATGCCCCAAAAGAGGTTAACCTTGATTTTCACACAAAAGAAGTCATTACAAACA GCATCACTCAACCTACCCTCCACAGTTTTGATGCTGCACAAAGCAGAGTGTATCAGCTCATGGAACAAGA CAGTTATACACGTTTTCTGAAATCTGACATCTATTTAGACTTGATGGAAGGAAGACCTCAGAGACCAACA AATCTTAGGAGACGATCACGCTCATTTACCTGCAATGAATTCCAAGATGTACAATCAGACGTTGCCATTT GGTTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204634 representing NM_130782
Red=Cloning site Green=Tags(s) METTLLFFSQINMCESKEKTFFKLIHGSGKEETSKEAKIRAKEKRNRLSLLVQKPEFHEDTRSSRSGHLA KETRVSPEEAVKWGESFDKLLSHRDGLEAFTRFLKTEFSEENIEFWIACEDFKKSKGPQQIHLKAKAIYE KFIQTDAPKEVNLDFHTKEVITNSITQPTLHSFDAAQSRVYQLMEQDSYTRFLKSDIYLDLMEGRPQRPT NLRRRSRSFTCNEFQDVQSDVAIWL TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_130782 |
ORF Size | 705 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_130782.1 |
RefSeq Size | 2158 bp |
RefSeq ORF | 708 bp |
Locus ID | 64407 |
UniProt ID | Q9NS28 |
Domains | RGS |
Gene Summary | This gene encodes a member of the regulator of G-protein signaling family. This protein is contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signaling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204634 | RGS18 (Myc-DDK-tagged)-Human regulator of G-protein signaling 18 (RGS18) |
CNY 2,400.00 |
|
RC204634L3 | Lenti ORF clone of Human regulator of G-protein signaling 18 (RGS18), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC204634L4 | Lenti ORF clone of Human regulator of G-protein signaling 18 (RGS18), mGFP tagged |
CNY 5,890.00 |
|
SC120407 | RGS18 (untagged)-Human regulator of G-protein signaling 18 (RGS18) |
CNY 3,990.00 |
|
SC321112 | RGS18 (untagged)-Human regulator of G-protein signaling 18 (RGS18) |
CNY 2,400.00 |