AGFG1 (NM_001135189) Human Tagged ORF Clone
CAT#: RC225895
AGFG1 (Myc-DDK-tagged)-Human ArfGAP with FG repeats 1 (AGFG1), transcript variant 4
ORF Plasmid: tGFP
"NM_001135189" in other vectors (4)
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CNY 3,888.00
CNY 4,470.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HRB; RAB; RIP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC225895 representing NM_001135189
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCAGCGCGAAGCGGAAGCAGGAGGAGAAGCACCTGAAGATGCTGCGGGACATGACCGGCCTCC CGCACAACCGAAAGTGCTTCGACTGCGACCAGCGCGGCCCCACCTACGTTAACATGACGGTCGGCTCCTT CGTGTGTACCTCCTGCTCCGGCAGCCTGCGAGGATTAAATCCACCACACAGGGTGAAATCTATCTCCATG ACAACATTCACACAACAGGAAATTGAATTCTTACAAAAACATGGAAATGAAGTCTGTAAACAGATTTGGC TAGGATTATTTGATGATAGATCTTCAGCAATTCCAGACTTCAGGGATCCACAAAAAGTGAAAGAGTTTCT ACAAGAAAAGTATGAAAAGAAAAGATGGTATGTCCCGCCAGAACAAGCCAAAGTCGTGGCATCAGTTCAT GCATCTATTTCAGGGTCCTCTGCCAGTAGCACAAGCAGCACACCTGAGGTCAAACCACTGAAATCTCTTT TAGGGGATTCTGCACCAACACTGCACTTAAATAAGGGCACACCTAGTCAGTCCCCAGTTGTAGGTCGTTC TCAAGGGCAGCAGCAGGAGAAGAAGCAATTTGACCTTTTAAGTGATCTCGGCTCAGACATCTTTGCTGCT CCAGCTCCTCAGTCAACAGCTACAGCCAATTTTGCTAACTTTGCACATTTCAACAGTCATGCAGGTGGAA GTGCTGCATCAGTAAATGCTAATTTTGCTCATTTTGATAACTTCCCCAAATCCTCCAGTGCTGATTTTGG AACCTTCAATACTTCCCAGAGTCATCAAACAGCATCAGCTGTTAGTAAAGTTTCAACGAACAAAGCTGGT TTACAGACTGCAGACAAATATGCAGCACTTGCTAATTTAGACAATATCTTCAGTGCCGGGCAAGGTGGTG ATCAGGGAAGTGGCTTTGGGACCACAGGTAAAGCTCCTGTTGGTTCTGTGGTTTCAGTTCCCAGTCAGTC AAGTGCATCTTCAGACAAGTATGCAGCTCTGGCAGAACTAGACAGCGTTTTCAGTTCTGCAGCCACCTCC AGTAATGCGTATACTTCCACAAGTAATGCTAGCAGCAATGTTTTTGGAACAGTGCCAGTGGTTGCTTCTG CACAGACACAGCCTGCTTCATCAAGTGTGCCTGCTCCATTTGGAGCTACGCCTTCCACAAATCCATTTGT TGCTGCTGCTGGTCCTTCTGTGGCATCTTCTACAAACCCATTTCAGACCAATGCCAGAGGAGCAACAGCG GCAACCTTTGGCACTGCATCCATGAGCATGCCCACAGGATTCGGCACTCCTGCTCCCTACAGTCTTCCCA CCAGCTTTAGTGGCAGCTTTCAGCAGCCTGCCTTTCCAGCCCAAGCAGCTTTCCCTCAACAGACAGCTTT TTCTCAACAGCCCAATGGTGCAGGTTTTGCAGCATTTGGACAAACAAAGCCAGTAGTAACCCCTTTTGGT CAAGTTGCAGCTGCTGGAGTATCTAGTAATCCTTTTATGACTGGTGCACCAACAGGACAATTTCCAACAG GAAGCTCATCAACCAATCCTTTCTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC225895 representing NM_001135189
Red=Cloning site Green=Tags(s) MAASAKRKQEEKHLKMLRDMTGLPHNRKCFDCDQRGPTYVNMTVGSFVCTSCSGSLRGLNPPHRVKSISM TTFTQQEIEFLQKHGNEVCKQIWLGLFDDRSSAIPDFRDPQKVKEFLQEKYEKKRWYVPPEQAKVVASVH ASISGSSASSTSSTPEVKPLKSLLGDSAPTLHLNKGTPSQSPVVGRSQGQQQEKKQFDLLSDLGSDIFAA PAPQSTATANFANFAHFNSHAGGSAASVNANFAHFDNFPKSSSADFGTFNTSQSHQTASAVSKVSTNKAG LQTADKYAALANLDNIFSAGQGGDQGSGFGTTGKAPVGSVVSVPSQSSASSDKYAALAELDSVFSSAATS SNAYTSTSNASSNVFGTVPVVASAQTQPASSSVPAPFGATPSTNPFVAAAGPSVASSTNPFQTNARGATA ATFGTASMSMPTGFGTPAPYSLPTSFSGSFQQPAFPAQAAFPQQTAFSQQPNGAGFAAFGQTKPVVTPFG QVAAAGVSSNPFMTGAPTGQFPTGSSSTNPFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001135189 |
ORF Size | 1566 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001135189.2 |
RefSeq ORF | 1569 bp |
Locus ID | 3267 |
UniProt ID | P52594 |
MW | 54 kDa |
Gene Summary | The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC225895L3 | Lenti-ORF clone of AGFG1 (Myc-DDK-tagged)-Human ArfGAP with FG repeats 1 (AGFG1), transcript variant 4 |
CNY 6,370.00 |
|
RC225895L4 | Lenti-ORF clone of AGFG1 (mGFP-tagged)-Human ArfGAP with FG repeats 1 (AGFG1), transcript variant 4 |
CNY 6,370.00 |
|
RG225895 | AGFG1 (tGFP-tagged) - Human ArfGAP with FG repeats 1 (AGFG1), transcript variant 4 |
CNY 4,940.00 |
|
SC326024 | AGFG1 (untagged)-Human ArfGAP with FG repeats 1 (AGFG1), transcript variant 4 |
CNY 8,360.00 |