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UPF2 (NM_080599) Human Tagged ORF Clone
CAT＃: RC215944
UPF2 (Myc-DDK-tagged)-Human UPF2 regulator of nonsense transcripts homolog (yeast) (UPF2), transcript variant 1
ORF Plasmid: tGFP
"NM_080599" in other vectors (6)
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CNY 8,856.00
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HUPF2; RENT2; smg-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215944 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCTGAGCGTAAAAAGCCAGCAAGTATGGAAGAAAAAGACTCTTTACCAAACAACAAGGAAAAAG ACTGCAGTGAAAGGCGGACAGTGAGCAGCAAGGAGAGGCCAAAAGACGATATCAAGCTCACTGCCAAGAA GGAGGTCAGCAAGGCCCCTGAAGACAAGAAGAAGAGACTGGAAGATGATAAGAGAAAAAAGGAAGACAAG GAACGCAAGAAAAAAGACGAAGAAAAGGTGAAGGCAGAGGAAGAATCAAAGAAAAAAGAAGAGGAAGAAA AAAAGAAACATCAAGAGGAAGAGAGAAAGAAGCAAGAAGAGCAGGCCAAACGTCAGCAAGAAGAAGAAGC AGCTGCTCAGATGAAAGAAAAAGAAGAATCCATTCAGCTTCATCAGGAAGCTTGGGAACGACATCATTTA AGAAAGGAACTTCGTAGCAAAAACCAAAATGCTCCGGACAGCCGACCAGAGGAAAACTTCTTCAGCCGCC TCGACTCAAGTTTGAAGAAAAATACTGCTTTTGTCAAGAAACTAAAAACTATTACAGAACAACAGAGAGA CTCCTTGTCCCATGATTTTAATGGCCTAAATTTAAGCAAATACATTGCAGAAGCTGTAGCTTCCATCGTG GAAGCAAAACTAAAAATCTCTGATGTGAACTGTGCTGTGCACCTCTGCTCTCTCTTTCACCAGCGTTATG CTGACTTTGCCCCATCACTTCTTCAGGTCTGGAAAAAACATTTTGAAGCAAGGAAAGAGGAGAAAACACC TAACATCACCAAGTTAAGAACTGATTTGCGTTTTATTGCAGAATTGACAATAGTTGGGATTTTCACTGAC AAGGAAGGTCTTTCCTTAATCTATGAACAGCTAAAAAATATTATTAATGCTGATCGGGAGTCCCACACTC ATGTCTCTGTAGTGATTAGTTTCTGTCGACATTGTGGAGATGATATTGCTGGACTTGTACCAAGGAAAGT AAAGAGTGCTGCAGAGAAGTTTAATTTGAGTTTTCCTCCTAGTGAGATAATTAGTCCAGAGAAACAACAG CCCTTCCAGAATCTTTTAAAAGAGTACTTTACGTCTTTGACCAAACACCTGAAAAGGGACCACAGGGAGC TCCAGAATACTGAGAGACAAAACAGGCGCATTCTACATTCTAAAGGGGAGCTCAGTGAAGATAGACATAA ACAGTATGAGGAATTTGCTATGTCTTACCAGAAGCTGCTGGCAAATTCTCAATCCTTAGCAGACCTTTTG GATGAAAATATGCCAGATCTTCCTCAAGACAAACCAACACCAGAAGAACATGGGCCTGGAATTGATATAT TCACACCTGGTAAACCTGGAGAATATGACTTGGAAGGTGGTATATGGGAAGATGAAGATGCTCGGAATTT TTATGAGAACCTCATTGATTTGAAGGCTTTTGTCCCAGCCATCTTGTTTAAAGACAATGAAAAAAGTTGT CAGAATAAAGAGTCCAACAAAGATGATACCAAAGAGGCAAAAGAATCTAAGGAGAATAAGGAGGTATCAA GTCCCGATGATTTGGAACTTGAGTTGGAGAATCTAGAAATTAATGATGACACCTTAGAATTAGAGGGTGG AGATGAAGCTGAAGATCTTACAAAGAAACTTCTTGATGAACAAGAACAAGAAGATGAGGAAGCCAGCACT GGATCTCATCTCAAGCTCATAGTAGATGCTTTCCTACAGCAGTTACCCAACTGTGTCAACCGAGATCTGA TAGACAAGGCAGCAATGGATTTTTGCATGAACATGAACACAAAAGCAAACAGGAAGAAGTTGGTACGGGC ACTCTTCATAGTTCCTAGACAAAGGTTGGATTTGCTACCATTTTATGCAAGATTGGTTGCTACATTGCAT CCCTGCATGTCTGATGTAGCAGAGGATCTTTGTTCCATGCTGAGGGGGGATTTCAGATTTCATGTACGGA AAAAGGACCAGATCAATATTGAAACAAAGAATAAAACTGTTCGTTTTATAGGAGAACTAACTAAGTTTAA GATGTTCACCAAAAATGACACACTGCATTGTTTAAAGATGCTTCTGTCAGACTTCTCTCATCACCATATT GAAATGGCATGCACCCTGCTGGAGACATGTGGACGGTTTCTTTTCAGATCTCCAGAATCTCACCTGAGGA CCAGTGTACTTTTGGAGCAAATGATGAGAAAGAAGCAAGCAATGCATCTTGATGCGAGATACGTCACAAT GGTAGAGAATGCATATTACTACTGCAACCCACCTCCAGCTGAAAAAACCGTGAAAAAGAAACGTCCTCCT CTCCAGGAATATGTCCGGAAACTTTTGTACAAGGATCTCTCTAAGGTTACCACCGAGAAGGTTTTGAGAC AGATGCGAAAGCTGCCCTGGCAGGACCAAGAAGTGAAAGACTATGTTATTTGTTGTATGATAAACATCTG GAATGTGAAATATAATAGTATTCATTGTGTAGCCAACCTCTTAGCAGGACTAGTGCTCTACCAAGAGGAT GTTGGGATCCACGTTGTGGATGGAGTGTTAGAAGATATTCGATTAGGAATGGAGGTTAATCAACCTAAAT TTAATCAGAGGCGCATCAGCAGTGCCAAGTTCTTAGGAGAACTTTACAATTACCGAATGGTGGAATCAGC TGTTATTTTCAGAACTCTGTATTCTTTTACCTCATTTGGTGTTAATCCTGATGGCTCTCCAAGTTCCCTG GACCCACCTGAGCATCTTTTCAGAATTAGACTCGTATGCACTATTCTGGACACATGTGGCCAGTACTTTG ACAGAGGTTCCAGTAAACGAAAACTTGATTGTTTCCTTGTATATTTTCAGCGTTATGTTTGGTGGAAGAA AAGTTTGGAGGTTTGGACAAAAGACCATCCATTTCCTATTGATATAGATTACATGATCAGTGATACACTA GAACTGCTAAGACCAAAGATCAAACTCTGTAATTCTCTGGAAGAATCCATCAGGCAGGTACAAGACTTGG AACGAGAATTCTTAATAAAACTAGGCCTAGTAAATGACAAAGACTCAAAAGATTCTATGACAGAAGGAGA AAATCTTGAAGAGGATGAAGAAGAAGAAGAAGGTGGGGCTGAAACAGAAGAACAATCTGGAAATGAAAGT GAAGTAAATGAGCCAGAAGAAGAGGAGGGTTCTGATAATGATGATGATGAGGGAGAAGAAGAGGAGGAAG AGAATACAGATTACCTTACAGATTCCAATAAGGAAAATGAAACCGATGAAGAGAATACTGAGGTAATGAT TAAAGGCGGTGGACTTAAGCATGTACCTTGTGTAGAAGATGAGGACTTCATTCAAGCTCTGGATAAAATG ATGCTAGAAAATCTACAGCAACGAAGTGGTGAATCTGTTAAAGTGCACCAACTAGATGTTGCCATTCCTT TGCATCTCAAAAGCCAGCTGAGGAAAGGGCCCCCACTGGGAGGTGGGGAAGGAGAGGCTGAGTCTGCAGA CACAATGCCGTTTGTCATGTTAACAAGAAAAGGCAATAAACAGCAGTTTAAGATCCTTAATGTACCCATG TCCTCTCAACTTGCTGCAAATCACTGGAACCAGCAACAGGCAGAACAAGAAGAGAGGATGAGAATGAAAA AGCTCACACTAGATATCAATGAACGGCAAGAACAAGAAGATTATCAAGAAATGTTGCAGTCTCTTGCACA GCGCCCAGCTCCAGCAAACACCAATCGTGAGAGGCGGCCTCGCTACCAACATCCGAAGGGAGCACCTAAT GCAGATCTAATCTTTAAGACTGGTGGGAGGAGACGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215944 protein sequence
Red=Cloning site Green=Tags(s) MPAERKKPASMEEKDSLPNNKEKDCSERRTVSSKERPKDDIKLTAKKEVSKAPEDKKKRLEDDKRKKEDK ERKKKDEEKVKAEEESKKKEEEEKKKHQEEERKKQEEQAKRQQEEEAAAQMKEKEESIQLHQEAWERHHL RKELRSKNQNAPDSRPEENFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNGLNLSKYIAEAVASIV EAKLKISDVNCAVHLCSLFHQRYADFAPSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIVGIFTD KEGLSLIYEQLKNIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKSAAEKFNLSFPPSEIISPEKQQ PFQNLLKEYFTSLTKHLKRDHRELQNTERQNRRILHSKGELSEDRHKQYEEFAMSYQKLLANSQSLADLL DENMPDLPQDKPTPEEHGPGIDIFTPGKPGEYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKSC QNKESNKDDTKEAKESKENKEVSSPDDLELELENLEINDDTLELEGGDEAEDLTKKLLDEQEQEDEEAST GSHLKLIVDAFLQQLPNCVNRDLIDKAAMDFCMNMNTKANRKKLVRALFIVPRQRLDLLPFYARLVATLH PCMSDVAEDLCSMLRGDFRFHVRKKDQINIETKNKTVRFIGELTKFKMFTKNDTLHCLKMLLSDFSHHHI EMACTLLETCGRFLFRSPESHLRTSVLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVKKKRPP LQEYVRKLLYKDLSKVTTEKVLRQMRKLPWQDQEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQED VGIHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFTSFGVNPDGSPSSL DPPEHLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLEVWTKDHPFPIDIDYMISDTL ELLRPKIKLCNSLEESIRQVQDLEREFLIKLGLVNDKDSKDSMTEGENLEEDEEEEEGGAETEEQSGNES EVNEPEEEEGSDNDDDEGEEEEEENTDYLTDSNKENETDEENTEVMIKGGGLKHVPCVEDEDFIQALDKM MLENLQQRSGESVKVHQLDVAIPLHLKSQLRKGPPLGGGEGEAESADTMPFVMLTRKGNKQQFKILNVPM SSQLAANHWNQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPN ADLIFKTGGRRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_080599 |
| ORF Size | 3816 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_080599.2, NP_542166.1 |
| RefSeq Size | 5387 bp |
| RefSeq ORF | 3819 bp |
| Locus ID | 26019 |
| UniProt ID | Q9HAU5 |
| MW | 147.8 kDa |
| Gene Summary | This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene; both variants encode the same protein. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215944L1 | Lenti ORF clone of Human UPF2 regulator of nonsense transcripts homolog (yeast) (UPF2), transcript variant 1, Myc-DDK-tagged |
CNY 11,256.00 |
|
| RC215944L2 | Lenti ORF clone of Human UPF2 regulator of nonsense transcripts homolog (yeast) (UPF2), transcript variant 1, mGFP tagged |
CNY 7,600.00 |
|
| RC215944L3 | Lenti ORF clone of Human UPF2 regulator of nonsense transcripts homolog (yeast) (UPF2), transcript variant 1, Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC215944L4 | Lenti ORF clone of Human UPF2 regulator of nonsense transcripts homolog (yeast) (UPF2), transcript variant 1, mGFP tagged |
CNY 11,256.00 |
|
| RG215944 | UPF2 (tGFP-tagged) - Human UPF2 regulator of nonsense transcripts homolog (yeast) (UPF2), transcript variant 1 |
CNY 6,270.00 |
|
| SC305799 | UPF2 (untagged)-Human UPF2 regulator of nonsense transcripts homolog (yeast) (UPF2), transcript variant 1 |
CNY 10,450.00 |
