Spastin (SPAST) (NM_199436) Human Tagged Lenti ORF Clone
CAT#: RC214100L4
- LentiORF®
Lenti ORF clone of Human spastin (SPAST), transcript variant 2, mGFP tagged
"NM_199436" in other vectors (4)
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CNY 6,460.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Synonyms | ADPSP; FSP2; SPG4 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC214100).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_199436 |
ORF Size | 1752 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_199436.1 |
RefSeq Size | 5125 bp |
RefSeq ORF | 1755 bp |
Locus ID | 6683 |
UniProt ID | Q9UBP0 |
Protein Families | Druggable Genome, Transmembrane |
MW | 63.4 kDa |
Gene Summary | This gene encodes a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. The use of alternative translational initiation sites in this gene results in a single transcript variant that can produce isoforms that differ in the length of their N-terminus and which thereby differ in the efficiency of their export from the nucleus to the cytoplasm. In addition, alternative splicing results in multiple transcript variants that encode isoforms that differ in other protein regions as well. One isoform of this gene has been shown to be a microtubule-severing enzyme that regulates microtubule abundance, mobility, and plus-end distribution. Mutations in this gene cause the most frequent form of autosomal dominant spastic paraplegia 4. [provided by RefSeq, May 2018] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214100 | SPAST (Myc-DDK-tagged)-Human spastin (SPAST), transcript variant 2 |
CNY 4,352.00 |
|
RC214100L3 | Lenti ORF clone of Human spastin (SPAST), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
RG214100 | SPAST (tGFP-tagged) - Human spastin (SPAST), transcript variant 2 |
CNY 5,952.00 |
|
SC307972 | SPAST (untagged)-Human spastin (SPAST), transcript variant 2 |
CNY 7,220.00 |
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