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KIF4A (NM_012310) Human Tagged ORF Clone
CAT＃: RC213971
- TrueORF®
KIF4A (Myc-DDK-tagged)-Human kinesin family member 4A (KIF4A). Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_012310" in other vectors (4)
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CNY 8,576.00
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | KIF4; KIF4G1; MRX100 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213971 ORF sequence, codon optimized.
Due to the complexity of NM_012310, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGAAGAGGTCAAGGGCATACCCGTGCGAGTTGCGTTGCGCTGTCGCCCACTGGTGCCAAAGGAAA TTTCTGAGGGATGCCAGATGTGTCTCTCCTTTGTCCCGGGGGAGCCGCAGGTCGTGGTCGGCACTGACAA GAGCTTTACATACGATTTCGTTTTTGACCCTAGCACAGAGCAGGAGGAGGTATTTAATACCGCGGTCGCA CCCCTCATAAAGGGAGTGTTTAAGGGCTACAACGCCACCGTGTTGGCTTACGGCCAGACCGGCAGTGGCA AGACATATTCTATGGGTGGGGCATACACCGCAGAACAGGAGAACGAACCAACCGTGGGGGTAATTCCTCG AGTAATCCAACTTCTTTTTAAGGAGATCGATAAAAAGAGTGACTTCGAATTTACCCTCAAAGTTAGTTAT CTGGAGATCTACAACGAGGAAATCCTGGATCTGCTGTGTCCCAGCAGGGAGAAAGCCCAGATCAATATTA GGGAGGACCCCAAGGAGGGGATCAAGATAGTGGGGCTGACCGAAAAGACAGTGCTCGTTGCTCTGGATAC CGTGAGCTGTTTGGAACAGGGGAACAATTCACGCACTGTCGCGTCCACCGCCATGAACTCTCAGTCTTCC CGCTCTCACGCAATTTTCACCATCAGTCTTGAGCAGCGGAAAAAGAGTGACAAGAATAGCAGCTTTAGGT CCAAGCTGCATCTGGTGGATTTGGCCGGCAGCGAGCGGCAAAAAAAGACCAAGGCTGAGGGCGACCGGCT GAAAGAGGGTATTAACATCAATCGGGGACTTCTGTGCTTGGGAAATGTAATCTCAGCCCTGGGTGACGAT AAGAAAGGAGGATTCGTACCATACCGCGACTCAAAACTCACCAGACTGCTGCAGGACTCACTGGGAGGAA ACTCACACACTCTGATGATCGCGTGTGTGTCTCCTGCTGATAGCAATCTCGAAGAAACTCTCAATACCCT GCGCTACGCCGACCGGGCACGCAAGATCAAAAACAAACCTATAGTGAACATCGACCCTCAGACAGCTGAG CTCAACCACCTGAAGCAGCAAGTGCAGCAGCTGCAGGTCTTGCTGCTGCAGGCCCACGGCGGCACGCTGC CTGGCAGCATTACTGTTGAACCAAGTGAAAACCTGCAGTCCTTGATGGAGAAGAACCAGTCTCTCGTTGA GGAAAACGAAAAGCTCAGCCGAGGGCTTTCCGAGGCCGCCGGTCAAACAGCACAAATGCTCGAAAGGATT ATTCTCACCGAGCAGGCCAATGAAAAGATGAACGCAAAACTTGAGGAGCTGAGACAGCACGCCGCCTGTA AACTGGACCTGCAAAAGCTGGTGGAGACTCTGGAGGACCAGGAGCTGAAGGAAAACGTGGAGATCATATG TAATCTGCAGCAACTGATCACACAGTTGAGCGACGAGACAGTTGCCTGTATGGCTGCTGCCATCGACACG GCAGTGGAGCAGGAAGCTCAGGTTGAGACTAGCCCCGAAACTAGCCGCAGTTCCGATGCGTTCACAACCC AGCACGCATTGCGACAGGCTCAGATGAGTAAGGAATTGGTGGAGTTGAACAAAGCTCTGGCTCTGAAGGA GGCCTTGGCGAGGAAGATGACCCAGAATGACTCCCAGCTGCAGCCCATCCAGTATCAGTACCAAGACAAC ATCAAAGAACTCGAGCTTGAAGTGATCAACCTTCAGAAGGAAAAAGAAGAGCTGGTCCTGGAGCTTCAAA CTGCAAAGAAAGATGCAAACCAGGCCAAACTGAGTGAAAGGCGACGCAAGAGATTGCAGGAGCTGGAGGG TCAGATTGCAGACCTGAAGAAAAAACTGAACGAACAGTCCAAGCTCCTGAAACTGAAGGAGAGCACAGAG CGGACCGTCTCAAAGCTGAATCAGGAGATCCGCATGATGAAAAACCAACGGGTACAGTTGATGCGGCAAA TGAAGGAAGACGCAGAGAAGTTCAGGCAGTGGAAGCAGAAAAAGGACAAAGAAGTGATCCAGCTGAAGGA AAGGGACCGGAAGAGGCAGTACGAGCTTCTCAAACTGGAGCGGAACTTTCAAAAACAGTCCAATGTCCTG CGGCGGAAAACCGAGGAAGCAGCAGCCGCAAACAAGCGGCTGAAAGATGCCTTGCAGAAACAGCGGGAAG TTGCGGACAAGCGAAAAGAGACTCAGAGCCGGGGCATGGAGGGAACCGCCGCTCGCGTAAAGAACTGGCT GGGCAATGAAATAGAGGTGATGGTGAGCACCGAGGAGGCCAAACGCCACCTCAATGACCTCCTCGAAGAT CGGAAAATCCTTGCACAAGACGTGGCCCAACTGAAGGAGAAAAAAGAATCCGGCGAAAACCCTCCCCCTA AGTTGCGGCGACGAACATTCAGCCTCACAGAGGTAAGGGGCCAGGTTAGCGAATCAGAAGACAGCATCAC GAAACAGATCGAATCCTTGGAAACCGAGATGGAATTTAGATCCGCACAGATCGCCGACCTGCAGCAAAAG CTCCTGGACGCCGAGAGTGAGGACAGGCCTAAACAAAGGTGGGAGAACATTGCTACAATACTCGAAGCCA AATGTGCCCTTAAATACTTGATCGGCGAGCTGGTTAGCAGCAAAATACAGGTGAGTAAGCTTGAGAGTTC TCTCAAGCAGAGCAAAACCTCCTGCGCAGATATGCAGAAAATGCTGTTCGAGGAAAGAAATCATTTCGCC GAGATTGAAACCGAGTTGCAAGCTGAGCTGGTTAGGATGGAACAGCAGCATCAGGAGAAAGTCCTGTATC TCCTGTCACAGCTCCAACAGAGCCAAATGGCAGAGAAGCAGCTGGAAGAGTCAGTGAGTGAGAAGGAGCA GCAGCTTCTGAGCACCCTGAAATGCCAAGACGAAGAACTCGAAAAGATGCGCGAAGTGTGCGAGCAGAAT CAGCAGCTCCTCAGGGAAAACGAAATCATTAAACAAAAACTCACATTGCTGCAGGTGGCTAGCAGACAGA AGCACTTGCCAAAAGATACGCTGCTGAGCCCAGACAGCTCTTTCGAGTATGTGCCTCCAAAACCTAAACC ATCCCGCGTGAAAGAGAAGTTTCTGGAACAGAGCATGGATATCGAGGATTTGAAGTATTGCTCCGAGCAT TCAGTCAATGAACATGAGGACGGTGATGGTGACGACGACGAGGGAGATGATGAAGAATGGAAACCGACCA AACTCGTCAAAGTTAGCCGCAAAAATATCCAAGGCTGTTCATGCAAAGGATGGTGTGGCAACAAGCAATG CGGTTGCAGGAAACAGAAATCTGATTGCGGTGTAGACTGTTGTTGCGATCCTACAAAATGCCGGAATCGC CAACAGGGAAAAGACAGCCTCGGTACAGTTGAGAGAACCCAGGATTCAGAGGGAAGCTTCAAACTCGAGG ATCCCACAGAGGTTACCCCAGGCCTGAGTTTCTTCAATCCCGTGTGCGCCACACCTAATAGTAAGATTCT GAAGGAGATGTGCGACGTGGAACAGGTTCTTTCCAAAAAAACCCCTCCCGCCCCATCTCCCTTTGATCTG CCTGAACTTAAGCATGTTGCTACTGAGTACCAAGAAAATAAGGCCCCTGGTAAGAAGAAGAAACGGGCTC TGGCCTCTAATACCTCCTTTTTCTCTGGCTGTTCCCCAATTGAGGAGGAGGCCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213971 representing NM_012310
Red=Cloning site Green=Tags(s) MKEEVKGIPVRVALRCRPLVPKEISEGCQMCLSFVPGEPQVVVGTDKSFTYDFVFDPSTEQEEVFNTAVA PLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQENEPTVGVIPRVIQLLFKEIDKKSDFEFTLKVSY LEIYNEEILDLLCPSREKAQINIREDPKEGIKIVGLTEKTVLVALDTVSCLEQGNNSRTVASTAMNSQSS RSHAIFTISLEQRKKSDKNSSFRSKLHLVDLAGSERQKKTKAEGDRLKEGININRGLLCLGNVISALGDD KKGGFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLNTLRYADRARKIKNKPIVNIDPQTAE LNHLKQQVQQLQVLLLQAHGGTLPGSITVEPSENLQSLMEKNQSLVEENEKLSRGLSEAAGQTAQMLERI ILTEQANEKMNAKLEELRQHAACKLDLQKLVETLEDQELKENVEIICNLQQLITQLSDETVACMAAAIDT AVEQEAQVETSPETSRSSDAFTTQHALRQAQMSKELVELNKALALKEALARKMTQNDSQLQPIQYQYQDN IKELELEVINLQKEKEELVLELQTAKKDANQAKLSERRRKRLQELEGQIADLKKKLNEQSKLLKLKESTE RTVSKLNQEIRMMKNQRVQLMRQMKEDAEKFRQWKQKKDKEVIQLKERDRKRQYELLKLERNFQKQSNVL RRKTEEAAAANKRLKDALQKQREVADKRKETQSRGMEGTAARVKNWLGNEIEVMVSTEEAKRHLNDLLED RKILAQDVAQLKEKKESGENPPPKLRRRTFSLTEVRGQVSESEDSITKQIESLETEMEFRSAQIADLQQK LLDAESEDRPKQRWENIATILEAKCALKYLIGELVSSKIQVSKLESSLKQSKTSCADMQKMLFEERNHFA EIETELQAELVRMEQQHQEKVLYLLSQLQQSQMAEKQLEESVSEKEQQLLSTLKCQDEELEKMREVCEQN QQLLRENEIIKQKLTLLQVASRQKHLPKDTLLSPDSSFEYVPPKPKPSRVKEKFLEQSMDIEDLKYCSEH SVNEHEDGDGDDDEGDDEEWKPTKLVKVSRKNIQGCSCKGWCGNKQCGCRKQKSDCGVDCCCDPTKCRNR QQGKDSLGTVERTQDSEGSFKLEDPTEVTPGLSFFNPVCATPNSKILKEMCDVEQVLSKKTPPAPSPFDL PELKHVATEYQENKAPGKKKKRALASNTSFFSGCSPIEEEAH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012310 |
| ORF Size | 3696 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012310.3, NM_012310.4, NP_036442.3 |
| RefSeq Size | 4388 bp |
| RefSeq ORF | 3699 bp |
| Locus ID | 24137 |
| UniProt ID | O95239 |
| Domains | kinesin |
| Protein Families | Druggable Genome |
| MW | 139.9 kDa |
| Gene Summary | This gene encodes a member of the kinesin 4 subfamily of kinesin related proteins. The encoded protein is an ATP dependent microtubule-based motor protein that is involved in the intracellular transport of membranous organelles. This protein also associates with condensed chromosome arms and may be involved in maintaining chromosome integrity during mitosis. This protein may also be involved in the organization of the central spindle prior to cytokinesis. A pseudogene of this gene is found on chromosome X.[provided by RefSeq, Mar 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213971L3 | Lenti-ORF clone of KIF4A (Myc-DDK-tagged)-Human kinesin family member 4A (KIF4A). Note: ORF is codon optimized |
CNY 14,160.00 |
|
| RC213971L4 | Lenti-ORF clone of KIF4A (mGFP-tagged)-Human kinesin family member 4A (KIF4A). Note: ORF is codon optimized |
CNY 14,160.00 |
|
| RG213971 | KIF4A (tGFP-tagged) - Human kinesin family member 4A (KIF4A). Note: ORF is codon optimized |
CNY 13,490.00 |
|
| SC115446 | KIF4A (untagged)-Human kinesin family member 4A (KIF4A) |
CNY 8,584.00 |
