NKX2.8 (NKX2-8) (NM_014360) Human Tagged ORF Clone
CAT#: RC207752
NKX2 (Myc-DDK-tagged)-Human NK2 homeobox 8 (NKX2-8)
ORF Plasmid: tGFP
"NM_014360" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Nkx2-9; NKX2.8; NKX2H |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207752 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCTCTGGACGCCTGAGCTTCACCGTGCGCAGCCTTCTAGATTTACCCGAGCAGGACGCGCAAC ACCTGCCGAGGCGGGAGCCAGAACCACGCGCCCCCCAGCCCGACCCCTGCGCCGCCTGGCTGGATTCGGA GCGCGGCCACTACCCTTCCTCGGACGAGAGCAGCCTGGAGACCAGCCCGCCAGACTCGTCGCAGCGGCCG TCCGCTAGGCCCGCGTCTCCGGGCTCGGACGCCGAGAAAAGGAAGAAGCGGCGGGTGCTATTCTCCAAGG CGCAGACGCTGGAGTTGGAGCGGCGCTTCCGGCAGCAGCGGTACCTGTCTGCGCCCGAGCGCGAGCAGCT GGCGAGCCTGCTTCGCCTCACGCCCACGCAGGTCAAGATCTGGTTCCAGAATCATCGCTACAAGCTGAAG CGCGCTCGCGCTCCAGGGGCGGCGGAGTCGCCTGACCTGGCAGCATCCGCCGAGCTGCACGCCGCGCCCG GCCTGCTGCGTCGCGTGGTGGTGCCGGTGCTTGTTCGCGACGGGCAGCCGTGCGGCGGCGGCGGCGGTGG CGAGGTGGGAACCGCCGCGGCCCAGGAGAAGTGCGGCGCCCCTCCAGCCGCCGCCTGCCCTCTGCCGGGC TACCCTGCCTTCGGTCCCGGCTCGGCGCTTGGCCTCTTCCCCGCCTACCAGCACTTAGCATCCCCCGCCC TGGTCTCCTGGAACTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207752 protein sequence
Red=Cloning site Green=Tags(s) MATSGRLSFTVRSLLDLPEQDAQHLPRREPEPRAPQPDPCAAWLDSERGHYPSSDESSLETSPPDSSQRP SARPASPGSDAEKRKKRRVLFSKAQTLELERRFRQQRYLSAPEREQLASLLRLTPTQVKIWFQNHRYKLK RARAPGAAESPDLAASAELHAAPGLLRRVVVPVLVRDGQPCGGGGGGEVGTAAAQEKCGAPPAAACPLPG YPAFGPGSALGLFPAYQHLASPALVSWNW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_014360 |
ORF Size | 717 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014360.4 |
RefSeq Size | 1857 bp |
RefSeq ORF | 720 bp |
Locus ID | 26257 |
UniProt ID | O15522 |
Protein Families | Druggable Genome, Transcription Factors |
MW | 25.9 kDa |
Gene Summary | The protein encoded by this gene is a homeobox-containing developmental regulator associated with liver development. The encoded protein binds to the alpha-fetoprotein (AFP) gene promoter and increases the expression of AFP. This gene is overexpressed in some lung cancers and is linked to poor patient survival, possibly due to its resistance to cisplatin. This gene is aberrantly methylated in pancreatic cancer, deleted in squamous cell lung carcinomas, and acts as a tumor suppressor in esophageal cancer. Mutations in this gene may also be a cause of neural tube defects. [provided by RefSeq, Dec 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207752L1 | Lenti ORF clone of Human NK2 homeobox 8 (NKX2-8), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC207752L2 | Lenti ORF clone of Human NK2 homeobox 8 (NKX2-8), mGFP tagged |
CNY 5,890.00 |
|
RC207752L3 | Lenti ORF clone of Human NK2 homeobox 8 (NKX2-8), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC207752L4 | Lenti ORF clone of Human NK2 homeobox 8 (NKX2-8), mGFP tagged |
CNY 5,890.00 |
|
RG207752 | NKX2 (tGFP-tagged) - Human NK2 homeobox 8 (NKX2-8) |
CNY 4,000.00 |
|
SC304088 | NKX2 (untagged)-Human NK2 homeobox 8 (NKX2-8) |
CNY 2,400.00 |