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STOM (NM_004099) Human Tagged ORF Clone
CAT＃: RC202060
STOM (Myc-DDK-tagged)-Human stomatin (STOM), transcript variant 1
ORF Plasmid: tGFP
"NM_004099" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BND7; EPB7; EPB72 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202060 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGAAGCGGCACACACGGGACTCCGAAGCCCAGCGGCTCCCCGACTCCTTCAAGGACAGCCCCA GTAAGGGCCTTGGACCTTGCGGATGGATTTTGGTGGCGTTCTCATTCTTATTCACCGTTATAACTTTCCC AATCTCAATATGGATGTGCATAAAGATTATAAAAGAGTATGAAAGAGCCATCATCTTTAGATTGGGTCGC ATTTTACAAGGAGGAGCCAAAGGACCTGGTTTGTTTTTTATTCTGCCATGCACTGACAGCTTCATCAAAG TGGACATGAGAACTATTTCATTTGATATTCCTCCTCAGGAGATCCTCACAAAGGATTCAGTGACAATTAG CGTGGATGGTGTGGTCTATTACCGCGTTCAGAATGCAACCCTGGCTGTGGCAAATATCACCAACGCTGAC TCAGCAACCCGTCTTTTGGCACAAACTACTCTGAGGAATGTTCTGGGCACCAAGAATCTTTCTCAGATCC TCTCTGACAGAGAAGAAATTGCACACAACATGCAGTCTACTCTGGATGATGCCACTGATGCCTGGGGAAT AAAGGTGGAGCGTGTGGAAATTAAGGATGTGAAACTACCTGTGCAGCTCCAGAGAGCTATGGCTGCAGAA GCAGAAGCGTCCCGCGAGGCCCGCGCCAAGGTTATTGCAGCCGAAGGAGAAATGAATGCATCCAGGGCTC TGAAAGAAGCCTCCATGGTCATCACTGAATATCCTGCAGCCCTTCAGCTCCGATACCTGCAGACACTGAC CACCATTGCTGCTGAGAAAAACTCAACAATTGTCTTCCCTCTGCCCATAGATATGCTGCAAGGAATCATA GGGGCAAAACACAGCCATCTAGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202060 protein sequence
Red=Cloning site Green=Tags(s) MAEKRHTRDSEAQRLPDSFKDSPSKGLGPCGWILVAFSFLFTVITFPISIWMCIKIIKEYERAIIFRLGR ILQGGAKGPGLFFILPCTDSFIKVDMRTISFDIPPQEILTKDSVTISVDGVVYYRVQNATLAVANITNAD SATRLLAQTTLRNVLGTKNLSQILSDREEIAHNMQSTLDDATDAWGIKVERVEIKDVKLPVQLQRAMAAE AEASREARAKVIAAEGEMNASRALKEASMVITEYPAALQLRYLQTLTTIAAEKNSTIVFPLPIDMLQGII GAKHSHLG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004099 |
| ORF Size | 864 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004099.2 |
| RefSeq Size | 3198 bp |
| RefSeq ORF | 867 bp |
| Locus ID | 2040 |
| UniProt ID | P27105 |
| Domains | Band_7 |
| Protein Families | Transmembrane |
| MW | 31.8 kDa |
| Gene Summary | This gene encodes a member of a highly conserved family of integral membrane proteins. The encoded protein localizes to the cell membrane of red blood cells and other cell types, where it may regulate ion channels and transporters. Loss of localization of the encoded protein is associated with hereditary stomatocytosis, a form of hemolytic anemia. There is a pseudogene for this gene on chromosome 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202060L3 | Lenti ORF clone of Human stomatin (STOM), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202060L4 | Lenti ORF clone of Human stomatin (STOM), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
| RG202060 | STOM (tGFP-tagged) - Human stomatin (STOM), transcript variant 1 |
CNY 4,000.00 |
|
| SC117590 | STOM (untagged)-Human stomatin (STOM), transcript variant 1 |
CNY 2,400.00 |
