CMBL (NM_138809) Human Tagged ORF Clone
CAT#: RC201711
CMBL (Myc-DDK-tagged)-Human carboxymethylenebutenolidase homolog (Pseudomonas) (CMBL)
ORF Plasmid: tGFP
"NM_138809" in other vectors (6)
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CNY 3,600.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | JS-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201711 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAACGAAGCTTATCCTTGTCCGTGTGACATTGGCCACAGACTTGAGTATGGAGGGCTAGGCCGTG AAGTTCAAGTCGAGCACATCAAGGCTTATGTCACCAAATCCCCCGTTGATGCAGGCAAAGCTGTGATTGT CATTCAAGATATATTTGGCTGGCAGTTGCCCAATACCAGATATATAGCTGACATGATCTCAGGAAATGGA TACACAACCATTGTTCCAGACTTCTTTGTAGGGCAAGAGCCTTGGGACCCCTCTGGCGACTGGTCTATCT TCCCTGAGTGGCTGAAAACAAGAAATGCCCAGAAGATCGATAGAGAGATCAGTGCTATCTTGAAGTATCT GAAACAACAGTGTCATGCCCAGAAAATTGGCATCGTGGGATTCTGCTGGGGTGGAACTGCTGTCCATCAT TTGATGATGAAATACTCAGAATTCAGGGCAGGGGTGTCCGTCTATGGCATTGTCAAGGATTCTGAAGACA TTTACAATTTAAAGAACCCCACTTTGTTCATTTTTGCTGAAAATGATGTTGTGATTCCACTCAAGGACGT ATCTTTGCTGACTCAGAAGTTGAAAGAACACTGCAAAGTTGAATATCAAATTAAAACATTTTCTGGGCAG ACTCATGGGTTCGTGCATCGGAAGAGAGAAGATTGCTCACCTGCAGACAAGCCCTACATTGACGAGGCCA GAAGGAATTTAATTGAGTGGCTGAACAAGTACATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201711 protein sequence
Red=Cloning site Green=Tags(s) MANEAYPCPCDIGHRLEYGGLGREVQVEHIKAYVTKSPVDAGKAVIVIQDIFGWQLPNTRYIADMISGNG YTTIVPDFFVGQEPWDPSGDWSIFPEWLKTRNAQKIDREISAILKYLKQQCHAQKIGIVGFCWGGTAVHH LMMKYSEFRAGVSVYGIVKDSEDIYNLKNPTLFIFAENDVVIPLKDVSLLTQKLKEHCKVEYQIKTFSGQ THGFVHRKREDCSPADKPYIDEARRNLIEWLNKYM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_138809 |
ORF Size | 735 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_138809.4 |
RefSeq Size | 4047 bp |
RefSeq ORF | 738 bp |
Locus ID | 134147 |
UniProt ID | Q96DG6 |
Domains | DLH |
Protein Pathways | Metabolic pathways |
MW | 28 kDa |
Gene Summary | CMBL (EC 3.1.1.45) is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL preferentially cleaves cyclic esters, and it activates medoxomil-ester prodrugs in which the medoxomil moiety is linked to an oxygen atom (Ishizuka et al., 2010 [PubMed 20177059]).[supplied by OMIM, Apr 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201711L1 | Lenti ORF clone of Human carboxymethylenebutenolidase homolog (Pseudomonas) (CMBL), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201711L2 | Lenti ORF clone of Human carboxymethylenebutenolidase homolog (Pseudomonas) (CMBL), mGFP tagged |
CNY 6,000.00 |
|
RC201711L3 | Lenti ORF clone of Human carboxymethylenebutenolidase homolog (Pseudomonas) (CMBL), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201711L4 | Lenti ORF clone of Human carboxymethylenebutenolidase homolog (Pseudomonas) (CMBL), mGFP tagged |
CNY 5,890.00 |
|
RG201711 | CMBL (tGFP-tagged) - Human carboxymethylenebutenolidase homolog (Pseudomonas) (CMBL) |
CNY 5,200.00 |
|
SC120612 | CMBL (untagged)-Human carboxymethylenebutenolidase homolog (Pseudomonas) (CMBL) |
CNY 3,600.00 |