Gls2 (NM_001285777) Mouse Tagged ORF Clone
CAT#: MR229778
- TrueORF®
Gls2 (myc-DDK-tagged) - Mouse glutaminase 2 (liver, mitochondrial) (Gls2), transcript variant 2
"NM_001285777" in other vectors (1)
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CNY 4,090.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | A330074B06Rik; AI195532; GA; GLS; Lga |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR229778 representing NM_001285777
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGATCGCATATTTGAGGATGCCAAAGAGCCCACTGGAGGCAAAGCACTCTGTGGGCCACACGAAGA TCCCCTTCTGCCTGCAGTCCTGTGTCAAGCCCCTCACTTACGCCATCTCCGTGAGCACCTTAGGCACTGA CTACGTGCACAAGTTTGTGGGCAAGGAACCCAGTGGTCTGCGCTATAACAAACTCTCCCTCAATGAGGAA GGAATTCCCCATAACCCCATGGTCAATGCTGGTGCCATTGTGGTCAGCTCCCTGATCAAGATGGACTGTA ACAAAGCAGAGAAGTTCGATTTTGTGTTACAGTATCTGAACAAGATGGCTGGGAACGAATTCATGGGGTT CAGCAATGCCACATTCCAGTCAGAGAAGGAGACTGGGGATCGGAATTACGCCATCGGCTATTACCTCAAG GAGAAGAAGTGCTTCCCTAAGGGTGTGGACATGATGGCTGCCCTTGATCTCTATTTCCAGCTGTGCTCTG TGGAGGTTACCTGTGAGTCAGGCAGTGTCATGGCGGCCACTCTGGCCAATGGCGGCATCTGCCCTATCAC AGGGGAGAGCGTGCTGAGCGCCGAGGCCGTGCGCAACACCCTCAGCCTCATGCATTCCTGTGGCATGTAT GACTTCTCGGGCCAGTTTGCCTTCCATGTGGGCCTGCCAGCCAAGTCAGCTGTGTCAGGAGCCATCCTCC TGGTTGTACCCAATGTCATGGGCATGATGTGTCTGTCGCCGCCGTTAGACAAGCTGGGGAACAGCCAAAG GGGCATCAACTTCTGCCAGAAGTTGGTGTCTCTCTTTAACTTCCACAACTATGACAACCTGCGGCACTGC GCTCGGAAGTTAGACCCACGGAGGGAAGGAGGAGAGGTTCGGAACAAGACCGTGGTGAACCTGCTATTTG CTGCATATAGTGGAGATGTCTCGGCCCTTCGAAGGTTTGCATTGTCGGCCATGGATATGGAGCAGAAGGA CTATGATTCCCGCACAGCCCTACATGTCGCTGCAGCTGAAGGGCACATTGAAGTTGTCAAATTTCTGATC GAGGCTTGCAAAGTGAATCCTTTTGTCAAGGACAGGTGGGGCAACATTCCCCTGGACGATGCTGTGCAGT TCAACCACCTGGAGGTGGTCAAACTGCTTCAAGATTACCATGACTCCTACTTGCTCTCTGAGACTCAAGC TGAGGCGGCAGCTGAGACCCTGTCCAAAGAGAATCTAGAGAGCATGGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR229778 representing NM_001285777
Red=Cloning site Green=Tags(s) MWIAYLRMPKSPLEAKHSVGHTKIPFCLQSCVKPLTYAISVSTLGTDYVHKFVGKEPSGLRYNKLSLNEE GIPHNPMVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEFMGFSNATFQSEKETGDRNYAIGYYLK EKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMY DFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSQRGINFCQKLVSLFNFHNYDNLRHC ARKLDPRREGGEVRNKTVVNLLFAAYSGDVSALRRFALSAMDMEQKDYDSRTALHVAAAEGHIEVVKFLI EACKVNPFVKDRWGNIPLDDAVQFNHLEVVKLLQDYHDSYLLSETQAEAAAETLSKENLESMV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001285777 |
ORF Size | 1239 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001285777.1, NP_001272706.1 |
RefSeq Size | 2380 bp |
RefSeq ORF | 1242 bp |
Locus ID | 216456 |
MW | 46.1 kDa |
Gene Summary | Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC227567 | Gls2 (untagged) - Mouse glutaminase 2 (liver, mitochondrial) (Gls2), transcript variant 2 |
CNY 3,900.00 |