Retreg1 (NM_001277318) Mouse Tagged ORF Clone
CAT#: MR229237
- TrueORF®
Fam134b (myc-DDK-tagged) - Mouse family with sequence similarity 134, member B (Fam134b), transcript variant 6
"NM_001277318" in other vectors (1)
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CNY 3,230.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1810015C04Rik; AU015349; Fam134b; Retreg1d |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR229237 representing NM_001277318
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTTCAGCATGTTTCTTCAAGAAATGTCTCTTTTTAAACAGCAGAGTCCTGGCAAGTTTTGCCTCC TGGTCTGCAGTGTGTGCACATTTTTTACAATCTTGGGAAGTTACATTCCTGGGGTCATACTCAGCTACCT TCTGTTACTGTTTGCTTTTTTGTGTCCACTGTTTAAATGTAATGATATTGGACAAAAAATATACAGCAAA GTCAAGTCCATTCTATTAAAACTAGATTTTGGAATTGGAGAATATATTAACCAGAAGAAACGTGAGAGAT CTGAAGCAGATAAAGAAAAAAGTCACAAGGATGACAGTGAATTAGACTTTTCAGCTCTTTGTCCTAAGAT TAGCCTCACAGTTGCTGCCAAAGAGTTATCTGTGTCAGACACAGATGTGTCAGAAGTCTCTTGGACTGAC AATGGGACCTTCAACCTTTCAGAGGGGTACACTCCGCAGACAGACACTTCTGACGATCTTGACCGGCCCA GTGAGGAAGTTTTCTCTCGAGATCTTTCAGATTTTCCATCTCTGGAAAATGGGACGGGAACAAATGATGA AGATGAATTAAGCCTGGGCTTACCCACAGAGCTCAAGAGAAAGAAGCAGCAGTTGGATAGTGCTCACAGA CCAAGCAAAGAACGGCAGTCGGCAGCTGGCCTCTCACTTCCTCTCAAGAGCGACCAAGCCCTTCACTTGA TGAGCAACCTGGCTGGGGATGTCATCACAGCCGCCATGACGGCTGCCATCAAAGACCAGCTAGAAGGAGC CCGGCAGGCACTCACTCAGGTGGCGCCCACTGCGGGAGAGGACACAGACACTGAGGAGGGGGATGACTTT GAACTACTTGACCAGGCAGAGCTAGATCAAATTGAGAGTGAGCTGGGACTAACACAAGACCAAGGGGCAG AAGCCCAGCAAAGTAAGAAATCCTCAGGCTTCCTCTCCAATCTGCTTGGAGGCCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR229237 representing NM_001277318
Red=Cloning site Green=Tags(s) MNFSMFLQEMSLFKQQSPGKFCLLVCSVCTFFTILGSYIPGVILSYLLLLFAFLCPLFKCNDIGQKIYSK VKSILLKLDFGIGEYINQKKRERSEADKEKSHKDDSELDFSALCPKISLTVAAKELSVSDTDVSEVSWTD NGTFNLSEGYTPQTDTSDDLDRPSEEVFSRDLSDFPSLENGTGTNDEDELSLGLPTELKRKKQQLDSAHR PSKERQSAAGLSLPLKSDQALHLMSNLAGDVITAAMTAAIKDQLEGARQALTQVAPTAGEDTDTEEGDDF ELLDQAELDQIESELGLTQDQGAEAQQSKKSSGFLSNLLGGH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001277318 |
ORF Size | 966 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001277318.1, NP_001264247.1 |
RefSeq Size | 2761 bp |
RefSeq ORF | 969 bp |
Locus ID | 66270 |
MW | 35.8 kDa |
Gene Summary | Endoplasmic reticulum-anchored autophagy receptor that mediates ER delivery into lysosomes through sequestration into autophagosomes (PubMed:26040720). Promotes membrane remodeling and ER scission via its membrane bending capacity and targets the fragments into autophagosomes via interaction with ATG8 family proteins (PubMed:26040720). Required for long-term survival of nociceptive and autonomic ganglion neurons (PubMed:19838196, PubMed:26040720).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC227026 | Fam134b (untagged) - Mouse family with sequence similarity 134, member B (Fam134b), transcript variant 6 |
CNY 3,140.00 |
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