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Npr1 (NM_008727) Mouse Tagged ORF Clone
CAT＃: MR226811
- TrueORF®
Npr1 (Myc-DDK-tagged) - Mouse natriuretic peptide receptor 1 (Npr1)
ORF Plasmid: tGFP
"NM_008727" in other vectors (6)
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CNY 11,040.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI893888; GC-A; NPR-A; NPRA; Pndr |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226811 representing NM_008727
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGGTTCCCGACGCGTCCGTCCGCGCCTAAGGGCGCTGCTGCTGCTACCGCCGCTGCTGCTGCTCC GAAGCGGCCACGCGAGCGACCTGACCGTGGCCGTGGTGCTGCCGCTGACCAACACCTCGTACCCGTGGTC TTGGGCGCGTGTAGGGCCGGCGGTGGAACTGGCTCTCGGGAGGGTGAAGGCTCGGCCGGACTTGCTGCCG GGTTGGACGG[AT]CCGTATGGTGCTGGGCAGCAGCGAGAACGCGGCGGGCGTCTGCTCCGACACCGCCG CACCGCTGGCCGCGGTGGATCTCAAGTGGGAGCACAGCCCCGCGGTGTTCCTGGGCCCCGGCTGCGTATA CTCTGCTGCCCCGGTGGGACGCTTCACCGCGCACTGGCGGGTGCCGCTGCTGACGGCTGGCGCCCCGGCT CTGGGCATCGGGGTGAAGGATGAGTACGCGTTAACCACCCGCACAGGACCCAGCCATGTCAAGCTGGGCG ACTTCGTGACGGCGCTGCATCGACGGCTGGGCTGGGAGCACCAGGCGCTGGTGCTCTATGCAGATCGGCT GGGCGACGACCGGCCGTGCTTCTTCATAGTGGAGGGGCTGTACATGCGGGTGCGTGAGCGACTCAACATC ACAGTAAATCACCAGGAGTTCGTCGAGGGCGACCCGGACCACTACACCAAGCTACTGCGG[AT]CCGTGC AGCGCAAGGGCAGAGTTATCTACATCTGCAGTTCTCCGGATGCCTTCAGGAATCTGATGCTTTTGGCCCT GGATGCTGGCCTGACTGGGGAGGACTATGTTTTCTTCCACCTGGATGTGTTTGGGCAAAGCCTTCAGGGT GCTCAGGGCCCTGTTCCCAGGAAGCCCTGGGAAAGAGACGATGGGCAGGATAGGAGAGCCCGCCAGGCCT TTCAGGCTGCCAAAATTATTACTTACAAAGAACCCGATAATCCTGAGTACTTGGAATTCCTGAAGCAGCT AAAACTCTTGGCTGACAAGAAATTCAACTTCACCATGGAGGATGGCCTGAAAAATATCATCCCAGCATCC TTCCATGACGGGCTCCTGCTCTATGTCCAGGCAGTGACAGAGACTCTGGCACAGGGGGGCACTGTCACTG ATGGAGAGAACATCACTCAGCGGATGTGGAACCGAAGCTTCCAAGGTGTGACAGGATACCTGAAAATTGA TAGAAATGGAGATCGGGACACTGATTTCTCCCTCTGGGATATGGACCCCGAGACAGGTGCCTTCAGGGTT GTCCTGAACTTTAATGGTACTTCCCAGGAGCTGATGGCTGTGTCAGAACACAGATTATACTGGCCTCTGG GATACCCACCTCCTGACATCCCTAAATGTGGCTTTGACAATGAGGACCCAGCCTGCAACCAAGACCACTT TTCCACACTGGAGGTTCTGGCTTTGGTGGGCAGCCTCTCTCTGGTTAGCTTTCTGATCGTGTCTTTCTTC ATATACAGGAAGATGCAGCTGGAAAAGGAGCTGGTCTCAGAGTTGTGGCGGGTGCGCTGGGAGGACTTGC AGCCCAGCAGCCTGGAGAGGCACCTTCGGAGCGCTGGCAGTCGGCTGACCCTGAGTGGGCGAGGCTCCAA TTATGGCTCCCTGCTAACCACGGAGGGCCAGTTCCAAGTCTTTGCCAAGACAGCATACTATAAGGGCAAC CTCGTGGCTGTGAAACGTGTGAACCGGAAACGCATTGAGTTGACACGAAAAGTCCTGTTTGAACTTAAAC ATATGCGGGATGTGCAGAATGAGCACTTGACCAGATTTGTGGGAGCTTGTACCGACCCTCCCAACATCTG TATCCTCACAGAGTACTGTCCCCGTGGGAGCCTACAGGACATTCTAGAGAATGAGAGTATTACCCTGGAC TGGATGTTTCGGTACTCACTCACCAATGACATTGTCAAGGGAATGCTCTTTCTACACAACGGGGCCATTG GTTCCCATGGGAACCTCAAGTCATCCAACTGCGTGGTAGATGGACGTTTTGTGTTAAAGATCACAGACTA TGGGCTCGAGAGCTTCAGAGACCCGGAGCCAGAGCAAGGACACACCCTCTTTGCCAAAAAACTGTGGACT GCACCTGAGCTCCTGCGAATGGCTTCCCCACCTGCCCGTGGCTCCCAAGCTGGGGATGTCTACAGTTTTG GTATCATCCTTCAGGAAATTGCCCTAAGAAGTGGGGTCTTCTATGTGGAAGGTTTGGACCTCAGCCCAAA AGAGATCATTGAGCGTGTGACTCGGGGCGAGCAGCCCCCATTCCGACCTTCCATGGATCTGCAGAGCCAC CTGGAGGAACTGGGGCAGCTGATGCAGAGGTGCTGGGCAGAGGATCCTCAGGAGCGGCCACCCTTTCAAC AGATCCGCCTGGCGCTGCGCAAGTTCAACAAGGAGAACAGCAGCAACATCCTGGACAACCTGCTGTCACG CATGGAACAGTACGCCAACAACCTGGAGGAACTGGTAGAGGAGAGAACACAGGCTTATCTGGAGGAGAAG CGCAAAGCTGAGGCCCTGCTTTACCAGATTCTGCCTCACTCTGTGGCTGAGCAGCTGAAGAGAGGCGAGA CAGTCCAGGCTGAGGCATTTGATAGTGTTACTATCTACTTCAGTGATATCGTGGGCTTTACAGCTCTTTC AGCAGAGAGCACACCCATGCAGGTGGTCACCCTGCTCAATGATCTGTACACCTGTTTTGATGCTGTCATA GACAACTTTGATGTGTACAAGGTAGAGACCATTGGTGATGCTTACATGGTGGTATCAGGGCTCCCAGTGA GGAATGGACAGCTCCATGCCCGAGAGGTAGCCCGAATGGCACTTGCACTGCTCGATGCTGTACGCTCCTT CCGCATCCGCCATAGGCCCCAGGAACAGCTGCGCTTGCGCATTGGAATTCACACAGGTCCCGTGTGTGCT GGTGTGGTAGGGCTAAAGATGCCCCGATACTGCCTCTTTGGAGACACAGTCAACACAGCTTCAAGAATGG AGTCTAATGGGGAAGCCCTCAGGATCCACTTGTCTTCGGAGACCAAGGCTGTGCTGGAAGAGTTCGATGG TTTTGAGCTGGAGCTCCGAGGTGACGTGGAAATGAAGGGCAAAGGCAAGGTTCGAACCTATTGGCTCCTC GGGGAGCGGGGATGCAGCACTCGAGGCACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR226811 representing NM_008727
Red=Cloning site Green=Tags(s) MPGSRRVRPRLRALLLLPPLLLLRSGHASDLTVAVVLPLTNTSYPWSWARVGPAVELALGRVKARPDLLP GWTXXRMVLGSSENAAGVCSDTAAPLAAVDLKWEHSPAVFLGPGCVYSAAPVGRFTAHWRVPLLTAGAPA LGIGVKDEYALTTRTGPSHVKLGDFVTALHRRLGWEHQALVLYADRLGDDRPCFFIVEGLYMRVRERLNI TVNHQEFVEGDPDHYTKLLRXXVQRKGRVIYICSSPDAFRNLMLLALDAGLTGEDYVFFHLDVFGQSLQG AQGPVPRKPWERDDGQDRRARQAFQAAKIITYKEPDNPEYLEFLKQLKLLADKKFNFTMEDGLKNIIPAS FHDGLLLYVQAVTETLAQGGTVTDGENITQRMWNRSFQGVTGYLKIDRNGDRDTDFSLWDMDPETGAFRV VLNFNGTSQELMAVSEHRLYWPLGYPPPDIPKCGFDNEDPACNQDHFSTLEVLALVGSLSLVSFLIVSFF IYRKMQLEKELVSELWRVRWEDLQPSSLERHLRSAGSRLTLSGRGSNYGSLLTTEGQFQVFAKTAYYKGN LVAVKRVNRKRIELTRKVLFELKHMRDVQNEHLTRFVGACTDPPNICILTEYCPRGSLQDILENESITLD WMFRYSLTNDIVKGMLFLHNGAIGSHGNLKSSNCVVDGRFVLKITDYGLESFRDPEPEQGHTLFAKKLWT APELLRMASPPARGSQAGDVYSFGIILQEIALRSGVFYVEGLDLSPKEIIERVTRGEQPPFRPSMDLQSH LEELGQLMQRCWAEDPQERPPFQQIRLALRKFNKENSSNILDNLLSRMEQYANNLEELVEERTQAYLEEK RKAEALLYQILPHSVAEQLKRGETVQAEAFDSVTIYFSDIVGFTALSAESTPMQVVTLLNDLYTCFDAVI DNFDVYKVETIGDAYMVVSGLPVRNGQLHAREVARMALALLDAVRSFRIRHRPQEQLRLRIGIHTGPVCA GVVGLKMPRYCLFGDTVNTASRMESNGEALRIHLSSETKAVLEEFDGFELELRGDVEMKGKGKVRTYWLL GERGCSTRGTRPLEQKLISEED SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-NotI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008727 |
| ORF Size | 3171 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008727.5, NP_032753.5 |
| RefSeq Size | 4065 bp |
| RefSeq ORF | 3174 bp |
| Locus ID | 18160 |
| UniProt ID | P18293 |
| MW | 119.1 kDa |
| Gene Summary | Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The Virtually Mature BNP (BNP1-32) is a Precursor for the More Effective BNP1-30
,Schwiebs, A;Wang, Y;Moore, AM;Zhu, X;Pankow, K;Siems, WE;Walther, T;,
Br. J. Pharmacol.
,PubMed ID 31691951
[NPR1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223390 | Npr1 (untagged) - Mouse natriuretic peptide receptor 1 (Npr1), (10ug) |
CNY 11,048.00 |
|
| MG226811 | Npr1 (tGFP-tagged) - Mouse natriuretic peptide receptor 1 (Npr1), (10ug) |
CNY 12,640.00 |
|
| MR226811L1 | Lenti ORF clone of Npr1 (Myc-DDK-tagged) - Mouse natriuretic peptide receptor 1 (Npr1) |
CNY 12,730.00 |
|
| MR226811L2 | Lenti ORF clone of Npr1 (mGFP-tagged) - Mouse natriuretic peptide receptor 1 (Npr1) |
CNY 13,440.00 |
|
| MR226811L3 | Lenti ORF clone of Npr1 (Myc-DDK-tagged) - Mouse natriuretic peptide receptor 1 (Npr1) |
CNY 12,730.00 |
|
| MR226811L4 | Lenti ORF clone of Npr1 (mGFP-tagged) - Mouse natriuretic peptide receptor 1 (Npr1) |
CNY 12,730.00 |
