Cldn11 (NM_008770) Mouse Tagged ORF Clone
CAT#: MR225523
- TrueORF®
Cldn11 (Myc-DDK-tagged) - Mouse claudin 11 (Cldn11)
ORF Plasmid: tGFP
"NM_008770" in other vectors (3)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Claudin-11; Claudin11; Osp; Ot; Otm |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR225523 representing NM_008770
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTAGCCACTTGCCTTCAGGTGGTGGGTTTCGTCACGAGCTTCGTGGGTTGGATTGGCATCATCGTCA CAACGTCCACCAATGACTGGGTGGTGACCTGCAGCTACACCATCCCCACCTGCCGAAAAATGGACGAACT GGGCTCCAAGGGCCTGTGGGCTGACTGCGTCATGGCCACTGGTCTCTACCACTGCAAACCCCTGGTGGAC ATCCTCATCCTTCCAGGCTACGTGCAGGCTTGTAGAGCCCTCATGATTGCTGCCTCCGTTCTGGGCCTGC CCGCCATCTTGCTGCTGTTGACAGTTCTCCCCTGCATCCGAATGGGCCACGAGCCTGGAGTGGCCAAGTA CAGGCGAGCCCAGCTGGCTGGGGTGCTCCTTATTCTGCTGGCTCTCTGCGCCATTGTCGCCACCATCTGG TTTCCTGTATGTGCCCACCGCGAGATCACCATCGTGAGCTTTGGCTACTCGCTGTACGCAGGTTGGATCG GTGCTGTGATGTGCCTGGTGGGTGGCTGTGTCATCGTCTGCTGCTCCGGGGATGCACAGTCATTTGGAGA AAACCGTTTCTATTACTCTTCTGGTTCCAGCTCGCCAACGCATGCCAAGAGTGCCCATGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225523 representing NM_008770
Red=Cloning site Green=Tags(s) MVATCLQVVGFVTSFVGWIGIIVTTSTNDWVVTCSYTIPTCRKMDELGSKGLWADCVMATGLYHCKPLVD ILILPGYVQACRALMIAASVLGLPAILLLLTVLPCIRMGHEPGVAKYRRAQLAGVLLILLALCAIVATIW FPVCAHREITIVSFGYSLYAGWIGAVMCLVGGCVIVCCSGDAQSFGENRFYYSSGSSSPTHAKSAHV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_008770 |
ORF Size | 621 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008770.3, NP_032796.1 |
RefSeq Size | 1872 bp |
RefSeq ORF | 624 bp |
Locus ID | 18417 |
UniProt ID | Q60771 |
MW | 22.6 kDa |
Gene Summary | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is a major component of CNS (central nervous system) myelin and plays an important role in regulating proliferation and migration of oligodendrocytes. The basal cell tight junctions in stria vascularis are primarily composed of this protein, and the gene-null mice suffer severe deafness. This protein is also an obligatory protein for tight junction formation and barrier integrity in the testis and the gene deficiency results in loss of the Sertoli cell epithelial phenotype in the testis. [provided by RefSeq, Aug 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG225523 | Cldn11 (tGFP-tagged) - Mouse claudin 11 (Cldn11), (10ug) |
CNY 2,850.00 |
|
MR225523L3 | Lenti ORF clone of Cldn11 (Myc-DDK-tagged) - Mouse claudin 11 (Cldn11) |
CNY 4,750.00 |
|
MR225523L4 | Lenti ORF clone of Cldn11 (mGFP-tagged) - Mouse claudin 11 (Cldn11) |
CNY 4,750.00 |