Myt1 (NM_001171616) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Nzf; NZF-; Nzf2; Nzf2a; Nzf2b; Nztf; Nztf2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR224914 representing NM_001171616 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTCAGAAAGTGATGACAAGCGAGCCCGCACACGGTCCAAGACCCTCCGAGGACCCCCAGAGACCA CAGGTGCAGACCTCAGTTGTCCTACCCCAGGATGTACTGGTTCAGGACATGTGCGGGGCAAGTACTCCAG ACATCGAAGCTTACAGAGTTGCCCTCTGGCCAAGAAGAGAAAACTGGAGGATGCTGAGACTGAGCACCTG GTATCCAAGAGGAAGTCACACCCTCTGAGGCTGGCTCTGGATGAGGGTTACCGCATGGACAGTGATGGCA GTGAGGATGCTGAAGTGAAGGACGTCTCTGTTTCAGATGAATCAGAAGGACCCCTGGAGGAGGCTGAGGC TGAGATGTCAGGACAGGAGGAGATTCATCACCCACAGACAGCTGAAGGAAAAAGCCTCATCAAGCCCCAT TTTGACTCCAACCCCACAAGCAGCCCTTCTGGCTTCTCCAAGAGCAGCTACAGTAGCTACCAGGGGATCA TTGCAACTTCCCTCCTAAATCTGGGCCAAATTGCTGAAGAGGCCCTTGTGAAGGAGGATTCCGTCTCAGT AGCTAAGTTGAGCCCCACTGTTGTTCATCAGCTTCAGGATGAGGCTGCAATGGGGGTCAACAGTGACGAG GGTGAAAAGGACCTCTTTATACAGCCAGAAGATGTGGAGGAGGTCATTGAAGTCACAAGTGAACGTTCCC AGGAGCCATGCCCCCAGTCTCTGAAGGATATGGTTAGTGAAGAGTCAAGTAAGCAGAAAGGAGTCCTAGG TCATGAGGAGGAGGGAGAGGAGGAGGAAGAAGATGAAGAAGAAGAGGATGAGGAGGAGGAGGAGGAGGGA GAGGAAGGAGAAGAAGAAGAGGAGGAGGAGGAGGAAGAAGAGGAGGAGGAAGATGAAGAGGAAGAGGAGG AGGAGGAGGAAGCAGCTCCAAATGTTATCTTTGGGGAAGACACCTCCCATACCTCTGTCCAGAAGGCGTC TCCTGAGTTCCGAGGCCCAGAGCTATCTAGTCCTAAACCTGAGTACTCAGTCATCGTGGAGGTTCGCTCT GATGATGACAAGGATGAGGATTCACGCTCCCAGAAGTCAGCAGTCACAGATGAATCAGAAATGTATGACA TGATGACCCGTGGGAATTTAGGCCTTCTGGAACAGGCCATTGCCCTGAAGGCTGAGCAGGTGCGAGCGGT CTGTGAGTCTGGCTGTCCACCTGCTGAGCAGGGCCATCTGGGCCCAGGAGAGCCAGGGAAAATGGCAAAG CCCCTGGACGTGGTGAGGAAGAGCTGCTACAGCAAAGATCCTTCCAGGGTGGAGAAGCGTGAAATCAAGT GTCCGACACCTGGCTGTGATGGCACTGGCCATGTTACTGGATTGTACCCTCATCACCGCAGCCTGTCTGG CTGTCCCCACAAGGATAGAATCCCTCCAGAGATCTTGGCCATGCATGAAAATGTACTGAAGTGCCCTACT CCTGGCTGCACGGGCCAAGGCCATGTGAACAGCAACCGCAATACTCACAGAAGGCCCATGTGTTTTGTGA AGCAGCTTGAGGTTCCACCTTATGGGAGCTACAGACCCAATGTGGCTCCCGCCACGCCCAGGGCCAACTT GGCCAAGGAGCTGGAGAAGTTCTCCAAAGTCACCTTTGACTACGCAAGTTTTGATGCTCAGGTTTTTGGC AAACGTATGCTTGCCCCAAAGATTCAGACCAGCGAAACCTCACCCAAAGCCTTCCAATGCTTCGATTACT CTCATGATGCTGAGGCTGCACACATGGCTGCCACAGCCATCCTGAACCTCTCCACTCGATGTTGGGAGAT GCCAGAGAACCTCAGCACAAAGCCACAGGACCTCCCCAGCAAGGCTGTGGACATTGAGGTAGATGAAAAT GGAACTCTGGACTTGAGCATGCATAAACATCGCAAGCGGGAAAACACTTTCCCCAGCAGTAGCAGCTGCA GTAGCAGCCCTGGTGTCAAGTCTCCTGATGTCTCTCAACGTCAGAGCAGTACCAGTGCCCCCAGCAGCTC TATGACCTCACCCCAGTCCAGCCAGGCCTCTCGCCAGGATGAGTGGGACCGTCCTCTAGACTACACCAAA CCTAGTCGGCTTCGAGAGGAAGAGCCTGAGGAGTCAGAGCCAGCAGCACACTCTTTTGCTTCTTCTGAAG CAGATGATCAGGAGGTGTCAGAAGAGAACTTTGAGGAGCGGAAGTATCCAGGGGAAGTCACCCTAACCAA CTTTAAGCTGAAGTTTCTTTCCAAGGACATAAAGAAGGAACTTCTCACCTGTCCCACCCCCGGCTGTGAC GGCAGTGGCCATATCACCGGGAACTATGCCTCCCATCGCAGCCTCTCTGGTTGCCCTCTTGCTGACAAGA GCCTCAGAAACCTCATGGCTGCCCACTCTGCTGACCTCAAGTGCCCCACACCTGGCTGTGATGGCTCTGG CCACATAACAGGGAACTATGCTTCACACCGGAGTTTGTCAGGCTGCCCACGTGCCAAGAAGAGTGGACTC AAGGTGGCGCCTACCAAGGATGACAAGGAGGACCCCGAGTTGATGAAGTGCCCAGTTCCAGGCTGTGTGG GGCTCGGCCACATCAGCGGCAAATATGCCTCTCACAGGAGTGCTTCTGGCTGCCCACTGGCTGCTCGGAG GCAGAAAGAGGGAGCTCTCAATGGTTCATCTTTCTCGTGGAAGTCGCTGAAGAATGAGGGCCCTACTTGC CCCACCCCAGGTTGCGACGGCTCTGGCCATGCCAACGGCAGTTTCCTCACCCACCGAAGTTTGTCTGGCT GTCCCAGAGCAACCTTTGCTGGAAAGAAAGGAAAACTCTCAGGGGATGAGATTCTCAGCCCAAAGTTCAA GACAAGTGATGTGCTGGAGAATGATGAGGAGATCAAGCAGCTAAATCAGGAGATACGAGACTTAAATGAG TCCAATTCGGAAATGGAGGCTGCCATGGTGCAGCTGCAGTCTCAGATCTCGTCCATGGAGAAGAACCTGA AGAACATCGAGGAGGAGAACAAGCTCATTGAGGAGCAGAATGAGGCCCTGTTTCTGGAATTGTCCGGGCT TAGCCAAGCCCTCATCCAAAGTCTTGCCAATATTCGCCTTCCACACATGGAACCAATATGTGAACAGAAT TTTGACGCCTATGTGAACACCCTCACTGACATGTACTCCAATCAGGACTGCTACCAGAATCCGGAGAACA AAGGCCTTCTGGAAACGATCAAGCAAGCTGTGAGGGGCATTCAGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR224914 representing NM_001171616 Red=Cloning site Green=Tags(s) MSSESDDKRARTRSKTLRGPPETTGADLSCPTPGCTGSGHVRGKYSRHRSLQSCPLAKKRKLEDAETEHL VSKRKSHPLRLALDEGYRMDSDGSEDAEVKDVSVSDESEGPLEEAEAEMSGQEEIHHPQTAEGKSLIKPH FDSNPTSSPSGFSKSSYSSYQGIIATSLLNLGQIAEEALVKEDSVSVAKLSPTVVHQLQDEAAMGVNSDE GEKDLFIQPEDVEEVIEVTSERSQEPCPQSLKDMVSEESSKQKGVLGHEEEGEEEEEDEEEEDEEEEEEG EEGEEEEEEEEEEEEEEDEEEEEEEEEAAPNVIFGEDTSHTSVQKASPEFRGPELSSPKPEYSVIVEVRS DDDKDEDSRSQKSAVTDESEMYDMMTRGNLGLLEQAIALKAEQVRAVCESGCPPAEQGHLGPGEPGKMAK PLDVVRKSCYSKDPSRVEKREIKCPTPGCDGTGHVTGLYPHHRSLSGCPHKDRIPPEILAMHENVLKCPT PGCTGQGHVNSNRNTHRRPMCFVKQLEVPPYGSYRPNVAPATPRANLAKELEKFSKVTFDYASFDAQVFG KRMLAPKIQTSETSPKAFQCFDYSHDAEAAHMAATAILNLSTRCWEMPENLSTKPQDLPSKAVDIEVDEN GTLDLSMHKHRKRENTFPSSSSCSSSPGVKSPDVSQRQSSTSAPSSSMTSPQSSQASRQDEWDRPLDYTK PSRLREEEPEESEPAAHSFASSEADDQEVSEENFEERKYPGEVTLTNFKLKFLSKDIKKELLTCPTPGCD GSGHITGNYASHRSLSGCPLADKSLRNLMAAHSADLKCPTPGCDGSGHITGNYASHRSLSGCPRAKKSGL KVAPTKDDKEDPELMKCPVPGCVGLGHISGKYASHRSASGCPLAARRQKEGALNGSSFSWKSLKNEGPTC PTPGCDGSGHANGSFLTHRSLSGCPRATFAGKKGKLSGDEILSPKFKTSDVLENDEEIKQLNQEIRDLNE SNSEMEAAMVQLQSQISSMEKNLKNIEEENKLIEEQNEALFLELSGLSQALIQSLANIRLPHMEPICEQN FDAYVNTLTDMYSNQDCYQNPENKGLLETIKQAVRGIQV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001171616
ORF Size	3267 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001171616.1, NP_001165087.1
RefSeq Size	5415 bp
RefSeq ORF	3270 bp
Locus ID	17932
UniProt ID	Q8CFC2
MW	120 kDa
Gene Summary	This gene is a member of the myelin transcription factor 1 gene family. The encoded protein, a zinc finger DNA-binding protein, is involved in regulation of oligodendrocyte differentiation and proliferation in the developing central nervous system. The gene product has a role in remyelination through regeneration of oligodendrocyte lineage cells in response to demyelination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010]

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