Tm4sf20 (NM_025453) Mouse Tagged ORF Clone
CAT#: MR224367
- TrueORF®
Tm4sf20 (Myc-DDK-tagged) - Mouse transmembrane 4 L six family member 20 (Tm4sf20)
ORF Plasmid: tGFP
"NM_025453" in other vectors (4)
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CNY 3,600.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1810018L02Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR224367 representing NM_025453
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTGCTGTGAAGGGTGGACATCCTGCAATGGCTTCAGCCTGCTCATTCTGATCCTGCTAGGAGTGG TTATCAATTGTATACCCCTGGGAATCAGCTTAGTGGAGGCAGACTCGACTTCTCAAAACCCCATCTCCTG CTATGAGTGGTGGTTTCCAGGAATTATAGGAGCAGGTCTGATGGCCATCCCAGCAACAACAATGTCCTTG GCAGCAAGAAAAAGAGCGTGCTGCAACAATAAGACTGGGATGTTTCTTTCATCACTCTTCAGTGTGATCA CAGTTGTTGGTGCTGTGTATTGCATGTTGGTATCACTCCAGGCTCTCTTGGAAGGACCTCTAATTTGTAA TACTCAGGCCAACAGTACTGTCACTTGTGAATTTTCATTGAAAAACTTAAGTAAATTTGATCCTGAATCC TTCAATCTGCTGTGGTTCTTCAATGGCACTTGTGTTTCTCCTACTGATTTTAAAAACCCCACCATCAATA ACATGGTCAGTAACTGGAAAATACCCAACTCCAACTCTGAAGAAGACAGACACAGGATTTTCCACTTCTC AGTATTTATGAGTCTCCTGCTTGTTGGAATCCTGGAGCTCCTGTTTGGGCTCAGTCAGATACTCATTGGT TTCCTTGGCTGTCTGTGTGGCGTCTCTCAGCGACGGAGTCAAATTGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224367 representing NM_025453
Red=Cloning site Green=Tags(s) MTCCEGWTSCNGFSLLILILLGVVINCIPLGISLVEADSTSQNPISCYEWWFPGIIGAGLMAIPATTMSL AARKRACCNNKTGMFLSSLFSVITVVGAVYCMLVSLQALLEGPLICNTQANSTVTCEFSLKNLSKFDPES FNLLWFFNGTCVSPTDFKNPTINNMVSNWKIPNSNSEEDRHRIFHFSVFMSLLLVGILELLFGLSQILIG FLGCLCGVSQRRSQIV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_025453 |
ORF Size | 678 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025453.4 |
RefSeq Size | 1503 bp |
RefSeq ORF | 681 bp |
Locus ID | 66261 |
UniProt ID | Q9CQY8 |
MW | 25.2 kDa |
Gene Summary | Polytopic transmembrane protein. Inhibits regulated intramembrane proteolysis (RIP) of CREB3L1, inhibiting its activation and the induction of collagen synthesis. In response to ceramide, which alters TM4SF20 membrane topology, stimulates RIP activation of CREB3L1. Ceramide reverses the direction through which transmembrane helices are translocated into the endoplasmic reticulum membrane during translation of TM4SF20, this mechanism is called 'regulated alternative translocation' (RAT) and regulates the function of the transmembrane protein.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210311 | Tm4sf20 (untagged) - Mouse transmembrane 4 L six family member 20 (Tm4sf20), (10ug) |
CNY 3,840.00 |
|
MG224367 | Tm4sf20 (tGFP-tagged) - Mouse transmembrane 4 L six family member 20 (Tm4sf20), (10ug) |
CNY 4,370.00 |
|
MR224367L3 | Lenti ORF clone of Tm4sf20 (Myc-DDK-tagged) - Mouse transmembrane 4 L six family member 20 (Tm4sf20) |
CNY 5,890.00 |
|
MR224367L4 | Lenti ORF clone of Tm4sf20 (mGFP-tagged) - Mouse transmembrane 4 L six family member 20 (Tm4sf20) |
CNY 5,890.00 |
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