Myt1 (NM_001171680) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Nzf; NZF-; Nzf2; Nzf2a; Nzf2b; Nztf; Nztf2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR223108 representing NM_001171680 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTCTTCAAGGCCCCGTTGTTTCTTGTTTTATGCAGGAAAAAGCCTCATCAAGCCCCATTTTGACT CCAACCCCACAAGCAGCCCTTCTGGCTTCTCCAAGAGCAGCTACAGTAGCTACCAGGGGATCATTGCAAC TTCCCTCCTAAATCTGGGCCAAATTGCTGAAGAGGCCCTTGTGAAGGAGGATTCCGTCTCAGTAGCTAAG TTGAGCCCCACTGTTGTTCATCAGCTTCAGGATGAGGCTGCAATGGGGGTCAACAGTGACGAGGGTGAAA AGGACCTCTTTATACAGCCAGAAGATGTGGAGGAGGTCATTGAAGTCACAAGTGAACGTTCCCAGGAGCC ATGCCCCCAGTCTCTGAAGGATATGGTTAGTGAAGAGTCAAGTAAGCAGAAAGGAGTCCTAGGTCATGAG GAGGAGGGAGAGGAGGAGGAAGAAGATGAAGAAGAAGAGGATGAGGAGGAGGAGGAGGAGGGAGAGGAAG GAGAAGAAGAAGAGGAGGAGGAGGAGGAAGAAGAGGAGGAGGAAGATGAAGAGGAAGAGGAGGAGGAGGA GGAAGCAGCTCCAAATGTTATCTTTGGGGAAGACACCTCCCATACCTCTGTCCAGAAGGCGTCTCCTGAG TTCCGAGGCCCAGAGCTATCTAGTCCTAAACCTGAGTACTCAGTCATCGTGGAGGTTCGCTCTGATGATG ACAAGGATGAGGATTCACGCTCCCAGAAGTCAGCAGTCACAGATGAATCAGAAATGTATGACATGATGAC CCGTGGGAATTTAGGCCTTCTGGAACAGGCCATTGCCCTGAAGGCTGAGCAGGTGCGAGCGGTCTGTGAG TCTGGCTGTCCACCTGCTGAGCAGGGCCATCTGGGCCCAGGAGAGCCAGGGAAAATGGCAAAGCCCCTGG ACGTGGTGAGGAAGAGCTGCTACAGCAAAGATCCTTCCAGGGTGGAGAAGCGTGAAATCAAGTGTCCGAC ACCTGGCTGTGATGGCACTGGCCATGTTACTGGATTGTACCCTCATCACCGCAGCCTGTCTGGCTGTCCC CACAAGGATAGAATCCCTCCAGAGATCTTGGCCATGCATGAAAATGTACTGAAGTGCCCTACTCCTGGCT GCACGGGCCAAGGCCATGTGAACAGCAACCGCAATACTCACAGAAGTTTATCTGGATGTCCCATTGCTGC TGCTGAAAAATTAGCCAAATCTCATGAGAAGCAGCAATTGCAGACAGGAGATCCTCCCAAAAATAACTCC AATTCAGATCGGATCCTCAGGCCCATGTGTTTTGTGAAGCAGCTTGAGGTTCCACCTTATGGGAGCTACA GACCCAATGTGGCTCCCGCCACGCCCAGGGCCAACTTGGCCAAGGAGCTGGAGAAGTTCTCCAAAGTCAC CTTTGACTACGCAAGTTTTGATGCTCAGGTTTTTGGCAAACGTATGCTTGCCCCAAAGATTCAGACCAGC GAAACCTCACCCAAAGCCTTCCAATGCTTCGATTACTCTCATGATGCTGAGGCTGCACACATGGCTGCCA CAGCCATCCTGAACCTCTCCACTCGATGTTGGGAGATGCCAGAGAACCTCAGCACAAAGCCACAGGACCT CCCCAGCAAGGCTGTGGACATTGAGGTAGATGAAAATGGAACTCTGGACTTGAGCATGCATAAACATCGC AAGCGGGAAAACACTTTCCCCAGCAGTAGCAGCTGCAGTAGCAGCCCTGGTGTCAAGTCTCCTGATGTCT CTCAACGTCAGAGCAGTACCAGTGCCCCCAGCAGCTCTATGACCTCACCCCAGTCCAGCCAGGCCTCTCG CCAGGATGAGTGGGACCGTCCTCTAGACTACACCAAACCTAGTCGGCTTCGAGAGGAAGAGCCTGAGGAG TCAGAGCCAGCAGCACACTCTTTTGCTTCTTCTGAAGCAGATGATCAGGAGGTGTCAGAAGAGAACTTTG AGGAGCGGAAGTATCCAGGGGAAGTCACCCTAACCAACTTTAAGCTGAAGTTTCTTTCCAAGGACATAAA GAAGGAACTTCTCACCTGTCCCACCCCCGGCTGTGACGGCAGTGGCCATATCACCGGGAACTATGCCTCC CATCGCAGCCTCTCTGGTTGCCCTCTTGCTGACAAGAGCCTCAGAAACCTCATGGCTGCCCACTCTGCTG ACCTCAAGTGCCCCACACCTGGCTGTGATGGCTCTGGCCACATAACAGGGAACTATGCTTCACACCGGAG TTTGTCAGGCTGCCCACGTGCCAAGAAGAGTGGACTCAAGGTGGCGCCTACCAAGGATGACAAGGAGGAC CCCGAGTTGATGAAGTGCCCAGTTCCAGGCTGTGTGGGGCTCGGCCACATCAGCGGCAAATATGCCTCTC ACAGGAGTGCTTCTGGCTGCCCACTGGCTGCTCGGAGGCAGAAAGAGGGAGCTCTCAATGGTTCATCTTT CTCGTGGAAGTCGCTGAAGAATGAGGGCCCTACTTGCCCCACCCCAGGTTGCGACGGCTCTGGCCATGCC AACGGCAGTTTCCTCACCCACCGAAGTTTGTCTGGCTGTCCCAGAGCAACCTTTGCTGGAAAGAAAGGAA AACTCTCAGGGGATGAGATTCTCAGCCCAAAGTTCAAGACAAGTGATGTGCTGGAGAATGATGAGGAGAT CAAGCAGCTAAATCAGGAGATACGAGACTTAAATGAGTCCAATTCGGAAATGGAGGCTGCCATGGTGCAG CTGCAGTCTCAGATCTCGTCCATGGAGAAGAACCTGAAGAACATCGAGGAGGAGAACAAGCTCATTGAGG AGCAGAATGAGGCCCTGTTTCTGGAATTGTCCGGGCTTAGCCAAGCCCTCATCCAAAGTCTTGCCAATAT TCGCCTTCCACACATGGAACCAATATGTGAACAGAATTTTGACGCCTATGTGAACACCCTCACTGACATG TACTCCAATCAGGACTGCTACCAGAATCCGGAGAACAAAGGCCTTCTGGAAACGATCAAGCAAGCTGTGA GGGGCATTCAGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR223108 representing NM_001171680 Red=Cloning site Green=Tags(s) MLSSRPRCFLFYAGKSLIKPHFDSNPTSSPSGFSKSSYSSYQGIIATSLLNLGQIAEEALVKEDSVSVAK LSPTVVHQLQDEAAMGVNSDEGEKDLFIQPEDVEEVIEVTSERSQEPCPQSLKDMVSEESSKQKGVLGHE EEGEEEEEDEEEEDEEEEEEGEEGEEEEEEEEEEEEEEDEEEEEEEEEAAPNVIFGEDTSHTSVQKASPE FRGPELSSPKPEYSVIVEVRSDDDKDEDSRSQKSAVTDESEMYDMMTRGNLGLLEQAIALKAEQVRAVCE SGCPPAEQGHLGPGEPGKMAKPLDVVRKSCYSKDPSRVEKREIKCPTPGCDGTGHVTGLYPHHRSLSGCP HKDRIPPEILAMHENVLKCPTPGCTGQGHVNSNRNTHRSLSGCPIAAAEKLAKSHEKQQLQTGDPPKNNS NSDRILRPMCFVKQLEVPPYGSYRPNVAPATPRANLAKELEKFSKVTFDYASFDAQVFGKRMLAPKIQTS ETSPKAFQCFDYSHDAEAAHMAATAILNLSTRCWEMPENLSTKPQDLPSKAVDIEVDENGTLDLSMHKHR KRENTFPSSSSCSSSPGVKSPDVSQRQSSTSAPSSSMTSPQSSQASRQDEWDRPLDYTKPSRLREEEPEE SEPAAHSFASSEADDQEVSEENFEERKYPGEVTLTNFKLKFLSKDIKKELLTCPTPGCDGSGHITGNYAS HRSLSGCPLADKSLRNLMAAHSADLKCPTPGCDGSGHITGNYASHRSLSGCPRAKKSGLKVAPTKDDKED PELMKCPVPGCVGLGHISGKYASHRSASGCPLAARRQKEGALNGSSFSWKSLKNEGPTCPTPGCDGSGHA NGSFLTHRSLSGCPRATFAGKKGKLSGDEILSPKFKTSDVLENDEEIKQLNQEIRDLNESNSEMEAAMVQ LQSQISSMEKNLKNIEEENKLIEEQNEALFLELSGLSQALIQSLANIRLPHMEPICEQNFDAYVNTLTDM YSNQDCYQNPENKGLLETIKQAVRGIQV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001171680
ORF Size	3024 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001171680.1, NP_001165151.1
RefSeq Size	5495 bp
RefSeq ORF	3027 bp
Locus ID	17932
MW	111.1 kDa
Gene Summary	This gene is a member of the myelin transcription factor 1 gene family. The encoded protein, a zinc finger DNA-binding protein, is involved in regulation of oligodendrocyte differentiation and proliferation in the developing central nervous system. The gene product has a role in remyelination through regeneration of oligodendrocyte lineage cells in response to demyelination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010]

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