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Copa (NM_009938) Mouse Tagged ORF Clone
CAT＃: MR222733
- TrueORF®
Copa (Myc-DDK-tagged) - Mouse coatomer protein complex subunit alpha (Copa)
ORF Plasmid: tGFP
"NM_009938" in other vectors (5)
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CNY 12,784.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AU040324; xenin |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222733 representing NM_009938
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAACCAAATTCGAGACGAAGAGCGCGCGGGTCAAAGGCCTTAGTTTTCATCCTAAAAGACCCTGGA TCCTGACCAGTTTACACAATGGGGTCATCCAGTTATGGGACTATCGGATGTGCACCCTAATTGACAAATT TGATGAACATGATGGTCCAGTGCGTGGCATTGACTTCCATAAGCAGCAGCCACTGTTCGTCTCTGGAGGC GATGATTATAAGATTAAGGTTTGGAATTACAAACTTCGGCGCTGCCTGTTCACATTGCTCGGGCACTTGG ATTATATCCGTACAACGTTTTTCCATCATGAATATCCTTGGATTCTGAGCGCCTCAGATGATCAGACCAT TCGGGTGTGGAACTGGCAGTCTAGAACCTGTGTCTGTGTGTTAACGGGGCACAACCATTATGTAATGTGT GCTCAGTTCCACCCCTCTGAAGACCTGGTGGTATCAGCTAGCCTGGACCAGACTGTCCGCGTTTGGGATA TTTCTGGTCTAAGGAAGAAAAACTTGTCTCCTGGTGCGGTGGAGTCGGATGTGAGAGGAATAACTGGCGT TGATCTGTTTGGAACCACGGATGCCGTGGTGAAGCATGTGCTAGAGGGTCATGACCGTGGAGTCAACTGG GCTGCCTTCCACCCGACTATGCCCCTTATTGTATCTGGGGCAGATGACCGGCAAGTGAAGATCTGGCGTA TGAATGAATCAAAGGCATGGGAGGTTGACACCTGCCGGGGCCATTACAACAATGTGTCTTGTGCTGTTTT TCACCCTCGCCAAGAGTTGATCCTCAGCAATTCTGAAGACAAGAGTATTCGAGTCTGGGATATGTCTAAG CGGACTGGGGTTCAGACTTTCCGTAGGGATCATGATCGCTTCTGGGTCCTGGCTGCCCATCCCAACCTTA ACCTCTTTGCAGCAGGCCATGATGGTGGGATGATTGTATTTAAGCTGGAACGTGAGCGTCCTGCCTATGC TGTTCATGGTAACATGCTGCATTATGTAAAGGACCGATTCCTCCGTCAGCTGGATTTCAACAGCTCCAAA GATGTAGCTGTGATGCAGTTGCGGAGCGGTTCTAAGTTTCCAGTATTCAACATGTCATACAATCCAGCAG AAAACGCAGTGCTGCTCTGCACCAGAGCAAGCAATCTAGAGAACAGTACCTACGACCTATACACCATCCC CAAAGACGCAGACTCCCAGAACCCTGACGCCCCTGAAGGGAAACGATCCTCAGGCCTGACAGCGGTTTGG GTTGCTCGGAATCGGTTTGCTGTCCTAGATCGCATGCATTCACTTCTGATTAAAAACCTGAAAAATGAGA TCACCAAGAAAATCCAGGTGCCAAACTGTGATGAAATCTTCTATGCTGGTACAGGCAACCTCCTGCTTCG GGATGCAGATTCCATCACACTCTTTGACGTCCAGCAGAAGCGAACTCTGGCATCAGTGAAGATTTCCAAG GTGAAATATGTTATCTGGTCGGCAGACATGTCTCATGTAGCGTTACTGGCCAAGCATGCCATTGTGATCT GTAACCGCAAGCTGGATGCTCTGTGTAACATTCATGAGAACATTCGTGTCAAGAGTGGGGCCTGGGATGA AAGTGGGGTGTTTATCTATACCACAAGCAACCACATCAAATATGCTGTCACCACTGGGGACCATGGGATT ATCCGAACTCTGGATTTACCCATCTATGTCACCAGAGTGAAGGGCAATAATGTATACTGCCTAGACAGAG AGTGTCGTCCTCGGGTGCTAACCATTGATCCCACTGAGTTCAAGTTCAAACTGGCCCTGATCAACAGAAA GTATGATGAGGTACTCCACATGGTGAGGAACGCTAAGCTGGTAGGCCAGTCAATCATCGCTTATCTCCAG AAGAAGGGCTATCCTGAAGTGGCGCTGCATTTTGTAAAGGATGAGAAAACTCGTTTCAGCCTGGCCCTGG AGTGTGGAAACATTGAGATTGCTCTAGAGGCAGCCAAAGCCCTGGACGACAAGAACTGCTGGGAGAAGCT AGGAGAAGTGGCCCTGCTACAGGGCAATCACCAAATTGTAGAGATGTGCTATCAGCGCACCAAAAACTTT GACAAACTTTCCTTCCTGTACCTTATTACTGGCAACCTGGAGAAACTTCGAAAGATGATGAAGATCGCTG AGATCCGAAAGGACATGAGTGGCCATTATCAGAATGCCCTATACCTAGGTGATGTGTCAGAGAGAGTACG GATCCTGAAGAACTGTGGGCAGAAATCCCTAGCCTATCTCTCAGCTGCTACCCATGGCTTAGATGAAGAA GCTGAGAGCCTGAAGGAAACCTTTGACCCTGAGAAGGAGACAATCCCAGACATTGATCCTAATGCCAAGC TGCTCCAGCCACCTGCACCTATCATGCCATTGGATACAAACTGGCCCTTACTGACCGTGTCCAAAGGGTT CTTTGAAGGCTCCATTGCAAGCAAGGGGAAGGGAGGAGCATTGGCTGCTGACATTGACATCGACACTGTA GGCACTGAAGGCTGGGGAGAGGACGCAGAGCTGCAGCTGGATGAAGATGGGTTTGTGGAGGCTCCAGAAG GTTTGGGAGAGGATGTTCTTGGAAAGGGACAAGAAGAAGGAGGTGGCTGGGATGTAGAAGAAGATTTGGA GCTCCCACCTGAGCTGGATGTACCCTCTGGGGTGTCTGGTAGTGCTGAAGATGGGTTCTTTGTGCCCCCA ACCAAGGGAACAAGCCCAACGCAAATCTGGTGCAATAACTCCCAGCTTCCAGTAGATCACATCTTGGCAG GTTCTTTTGAAACAGCCATGCGGCTTCTCCATGATCAGGTGGGAGTAATCCAGTTTGGCCCCTATAAGCA ACTGTTCCTACAGACATATGCCCGAGGCCGCACCACCTATCAAGCGCTGCCCTGCTTGCCCTCCATGTAT AGTTACCCTAATCGCAATTGGAAGGATGCAGGGCTAAAGAATGGAGTACCAGCTGTAGGCCTAAAGCTTA ATGACCTGATCCAACGTTTGCAACTGTGTTATCAGCTCACCACAGTTGGCAAGTTTGAGGAAGCTGTGGA AAAATTCCGTTCCATCCTACTCAGTGTGCCTCTTCTTGTTGTGGACAATAAACAGGAGATTGCAGAGGCC CAGCAGCTCATCACCATTTGCCGTGAATACATTGTGGGTTTGTGCATGGAGATAGAAAGGAAGAAGCTAC CTAAGGAGACCCTAGACCAGCAGAAGCGCATCTGTGAGATGGCAGCCTACTTCACACACTCAAATCTGCA GCCTGTGCATATGATCTTGGTGCTGCGGACAGCCCTCAACCTTTTCTTCAAGCTCAAGAACTTCAAGACA GCTGCCACCTTTGCCCGACGCCTGCTGGAACTTGGGCCCAAGCCTGAAGTAGCTCAGCAGACAAGAAAAA TCTTATCTGCCTGTGAGAAAAACCCCACAGATGCCTGCCAGCTCAATTATGACATGCACAACCCCTTTGA CATTTGTGCTGCATCTTACCGGCCCATCTACCGTGGAAAACCAGTGGAGAAGTGTCCACTCAGTGGGGCC TGCTATTCCCCTGAGTTCAAGGGGCAAATCTGCAGGGTCACTACAGTGACAGAGATTGGCAAAGATGTGA TTGGGCTGAGGATCAGTCCTCTTCAGTTTCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222733 representing NM_009938
Red=Cloning site Green=Tags(s) MLTKFETKSARVKGLSFHPKRPWILTSLHNGVIQLWDYRMCTLIDKFDEHDGPVRGIDFHKQQPLFVSGG DDYKIKVWNYKLRRCLFTLLGHLDYIRTTFFHHEYPWILSASDDQTIRVWNWQSRTCVCVLTGHNHYVMC AQFHPSEDLVVSASLDQTVRVWDISGLRKKNLSPGAVESDVRGITGVDLFGTTDAVVKHVLEGHDRGVNW AAFHPTMPLIVSGADDRQVKIWRMNESKAWEVDTCRGHYNNVSCAVFHPRQELILSNSEDKSIRVWDMSK RTGVQTFRRDHDRFWVLAAHPNLNLFAAGHDGGMIVFKLERERPAYAVHGNMLHYVKDRFLRQLDFNSSK DVAVMQLRSGSKFPVFNMSYNPAENAVLLCTRASNLENSTYDLYTIPKDADSQNPDAPEGKRSSGLTAVW VARNRFAVLDRMHSLLIKNLKNEITKKIQVPNCDEIFYAGTGNLLLRDADSITLFDVQQKRTLASVKISK VKYVIWSADMSHVALLAKHAIVICNRKLDALCNIHENIRVKSGAWDESGVFIYTTSNHIKYAVTTGDHGI IRTLDLPIYVTRVKGNNVYCLDRECRPRVLTIDPTEFKFKLALINRKYDEVLHMVRNAKLVGQSIIAYLQ KKGYPEVALHFVKDEKTRFSLALECGNIEIALEAAKALDDKNCWEKLGEVALLQGNHQIVEMCYQRTKNF DKLSFLYLITGNLEKLRKMMKIAEIRKDMSGHYQNALYLGDVSERVRILKNCGQKSLAYLSAATHGLDEE AESLKETFDPEKETIPDIDPNAKLLQPPAPIMPLDTNWPLLTVSKGFFEGSIASKGKGGALAADIDIDTV GTEGWGEDAELQLDEDGFVEAPEGLGEDVLGKGQEEGGGWDVEEDLELPPELDVPSGVSGSAEDGFFVPP TKGTSPTQIWCNNSQLPVDHILAGSFETAMRLLHDQVGVIQFGPYKQLFLQTYARGRTTYQALPCLPSMY SYPNRNWKDAGLKNGVPAVGLKLNDLIQRLQLCYQLTTVGKFEEAVEKFRSILLSVPLLVVDNKQEIAEA QQLITICREYIVGLCMEIERKKLPKETLDQQKRICEMAAYFTHSNLQPVHMILVLRTALNLFFKLKNFKT AATFARRLLELGPKPEVAQQTRKILSACEKNPTDACQLNYDMHNPFDICAASYRPIYRGKPVEKCPLSGA CYSPEFKGQICRVTTVTEIGKDVIGLRISPLQFR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009938 |
| ORF Size | 3672 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009938.4, NP_034068.3 |
| RefSeq Size | 4629 bp |
| RefSeq ORF | 3675 bp |
| Locus ID | 12847 |
| UniProt ID | Q8CIE6 |
| MW | 138.9 kDa |
| Gene Summary | The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222733 | Copa (tGFP-tagged) - Mouse coatomer protein complex subunit alpha (Copa), (10ug) |
CNY 13,490.00 |
|
| MR222733L1 | Lenti ORF clone of Copa (Myc-DDK-tagged) - Mouse coatomer protein complex subunit alpha (Copa) |
CNY 14,160.00 |
|
| MR222733L2 | Lenti ORF clone of Copa (mGFP-tagged) - Mouse coatomer protein complex subunit alpha (Copa) |
CNY 14,160.00 |
|
| MR222733L3 | Lenti ORF clone of Copa (Myc-DDK-tagged) - Mouse coatomer protein complex subunit alpha (Copa) |
CNY 14,160.00 |
|
| MR222733L4 | Lenti ORF clone of Copa (mGFP-tagged) - Mouse coatomer protein complex subunit alpha (Copa) |
CNY 14,160.00 |
