Myh8 (NM_177369) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4832426G23Rik; AI327267; MHCp; Myh; MyHC-; MyHC-pn; Myhs; Myhs-p; Myhsp
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR220412 representing NM_177369 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGCGGGTTCGGACGCGGAGATGGCTATTTTTGGCGAAGCTGCTCCTTACCTCCGAAAATCGGAGA AGGAGAGGATTGAGGCCCAAAATAAGCCTTTTGACGCAAAAACATCTGTCTTTGTGGCGGAGCCCAAGGA GTCTTATGTGAAAAGCGTTATACAAAGCAAGGATGGAGGGAAAGTGACCGTGAAGACGGAAAGTGGAGCA ACTCTCACCGTCAAGGAAGATCAAGTCTTCCCCATGAACCCTCCCAAATATGACAAGATCGAGGACATGG CCATGATGACCCACCTCCATGAGCCCGGAGTGCTGTACAACCTCAAAGAGCGTTATGCAGCCTGGATGAT CTATACCTACTCAGGCCTCTTCTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAACCCTGAG GTGGTGGCAGCCTACCGAGGCAAAAAGCGCCAGGAGGCCCCACCCCACATCTTCTCCATCTCTGACAACG CCTACCAGTTCATGCTGACGGATCGTGAGAACCAGTCCATCCTGATCACCGGAGAATCCGGGGCCGGGAA GACTGTGAACACGAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTCACTGGGGACAAGAAGAAGGAG GAGTCTGGCAAAATGCAGGGGACCCTGGAAGATCAAATCATCAGTGCTAACCCCCTGCTGGAGGCCTTTG GGAACGCCAAGACCGTGAGGAATGACAACTCATCTCGCTTTGGCAAGTTCATCAGAATCCACTTCGGTAC CACGGGGAAACTGGCATCAGCTGATATTGAAACCTATCTTCTAGAGAAGTCTAGAGTCACTTTCCAGCTA AAAGCTGAAAGAAGTTACCACATTTTTTATCAGATCACCTCCAATAAGAAGCCAGAGCTAATTGAAATGC TCCTGATCACCACCAACCCGTATGACTACGCCTTTGTCAGTCAGGGGGAGATCACGGTGCCCAGCATTGA TGACCAAGAAGAGTTGATGGCCACAGATAGTGCCATTGATATCCTTGGCTTTTCACCTGAAGAGAAAGTG TCCATCTATAAACTCACAGGGGCTGTGATGCATTATGGGAACATGAAGTTCAAGCAAAAGCAGCGGGAGG AGCAGGCTGAGCCAGATGGCACTGAAGTGGCTGACAAGGCTGCCTATCTCCAGTGTCTGAACTCTGCTGA TCTGCTCAAAGCCCTCTGCTATCCCAGGGTCAAGGTTGGCAATGAATATGTCACCAAAGGCCAGACGGTT CAACAAGTGTATAATGCGGTGGGTGCCCTGGCCAAGGCTGTCTATGAGAAGATGTTCCTGTGGATGGTCA CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCCGGCAGTACTTCATCGGGGTCTTGGACATCGCTGG CTTTGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAATGAGAAACTGCAACAG TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGACCTTCA TCGACTTCGGGATGGACCTGGCGGCCTGCATCGAGCTCATTGAGAAGCCACTGGGCATCTTCTCCATCCT GGAAGAAGAGTGCATGTTCCCTAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTACGACCAACATCTG GGAAAATCCAACAACTTCCAGAAGCCCAAGCCTACCAAAGGCAAGGCCGAGGCCCACTTCTCCCTGGTGC ACTATGCGGGCACTGTGGATTACAACATCACCGGCTGGCTGGACAAGAACAAGGACCCCCTGAATGACAC CGTGGTGGGGCTGTACCAGAAGTCAGCAATGAAAACTCTGGCCAGCCTCTTTTCCACATACGCTAGTGCT GAAGCAGATGGTGGTGCAAAGAAAGGAGCTAAGAAGAAGGGCTCTTCTTTCCAAACTGTGTCAGCCCTTT TCAGGGAAAACTTAAATAAACTGATGACCAATCTGAGAAGTACACACCCTCACTTCGTACGGTGTATCAT TCCCAATGAAACGAAGACTCCCGGTGCCATGGAGCACGAACTGGTCCTGCACCAGCTGAGGTGTAACGGG GTGCTGGAGGGCATCCGCATCTGCAGGAAGGGGTTCCCCAGCCGGATCCTGTATGGGGACTTTAAGCAGA GATACAAGGTTTTAAATGCAAGTGCTATTCCAGAGGGACAGTTCATCGACAGCAAGAAGGCCTCTGAGAA GCTTCTGGGCTCTATTGATATCGATCACACTCAGTATAAATTTGGACACACCAAGGTTTTCTTCAAAGCT GGTCTCCTGGGTCTCCTGGAAGAAATGAGAGATGAAAAGCTGGCCCAGATTATAACAAGAACCCAGGCGG TCTGTAGGGGATACCTAATGAGAGTGGAGTATCAGAAGATGCTGCTGAGAAGAGAATCCATCTTCTGTAT CCAGTACAACGTCCGCGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTCTTCAAGATCAAA CCCCTGCTGAAGAGCGCAGAGACCGAGAAGGAGATGGCCACCATGAAGGAGGAGTTCCAGAAAACCAAGG ATGAGCTGGCCAAGTCGGAGGCAAAGCGGAAGGAGCTGGAGGAGAAGATGGTCACTCTTCTCAAAGAGAA AAATGACCTGCAACTCCAGGTTCAATCTGAAGCAGACAGCTTGGCCGATGCTGAGGAAAGGTGTGAACAA TTGATCAAAAACAAAATCCAGCTGGAGGCCAAAATCAAAGAGGTGACCGAGAGGGCCGAGGACGAGGAGG AGATCAACGCGGAGCTCACGGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCAGAGCTGAAGAAGGACAT CGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACGGAGAACAAGGTGAAAAAC CTGACAGAGGAGATGGCAGGCCTGGATGAAAATATCGCCAAGCTGACCAAGGAGAAGAAGGCCCTCCAGG AGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCTGACCAAAGCCAA AACCAAGCTGGAACAGCAAGTGGATGACCTTGAGGGATCGCTAGAGCAAGAAAAGAAGCTGAGGATGGAC TTAGAAAGAGCCAAGAGGAAACTGGAGGGGGACCTGAAACTGGCCCAAGAGTCCACAATGGACATAGAAA ACGATAAACAGCAGCTTGATGAGAAACTCAAAAAGAAGGAGTTTGAAATCAGCAACCTGATAAGCAAAAT TGAAGATGAGCAAGCTGTGGAGATCCAGCTGCAGAAGAAGATCAAAGAGTTGCAGGCCCGCATCGAGGAG CTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCAGGGCCAAAGCAGAGAAGCAGCGCTCTGACCTCTCCC GGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCCGGCGGGGCCACCTCTGCTCAGGTGGAGATGAA CAAGAAGCGGGAAACTGAGTTTCAGAAACTTCGCCGGGACCTGGAGGAGGCCACGCTGCAGCACGAAGCC ACAGCAGCCGCTCTTCGGAAGAAGCACGCGGACAGTGTGGCTGAGCTCGGGGAGCAGATCGACAACCTGC AGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGCTGAAGATGGAGATCGATGACCTTAGCAGTAA TGCAGAGGCCATCGCCAAAGCCAAGGGAAATCTGGAGAAGATGTGCCGCACTCTGGAGGACCAGGTGAGT GAACTGAAGAGCAAGGAGGAGGAGCAGCAGCGGCTCATCAACGAGCTGACAGCGCAGAGAGCACGCCTGC AGACAGAAGCAGGTGAGTACTCCAGACAGCTGGATGAGAAAGATGCTTTAGTCTCTCAGTTGTCAAGGAG CAAGCAGGCATCCACGCAGCAGATTGAGGAGCTGAAGCGTCAGCTCGAGGAAGAAACCAAGGCCAAGAAC GCGCTGGCCCACGCCCTGCAGTCCTCCCGCCACGACTGTGATCTGCTGCGGGAACAGTATGAAGAGGAGC AGGAAGGCAAAGCCGAGCTGCAGAGGGCACTGTCCAAGGCCAACAGCGAGGTGGCCCAGTGGAGGACCAA ATATGAGACCGATGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAGAAGCTGGCTCAGCGTCTG CAGGCGGCCGAGGAGCACGTAGAAGCCGTGAACGCCAAGTGCGCTTCCCTGGAGAAGACGAAGCAGCGGC TGCAGAACGAGGTGGAGGACCTCATGATTGATGTGGAGAGGACCAACGCGGCCTGCGCCGCCCTGGACAA GAAGCAGAGAAACTTCGACAAGGTTCTATCAGAATGGAGACAGAAGTATGAGGAAACTCAGGCTGAGCTT GAGTCCTGCCAGAAGGAGTCCCGAACTCTCAGCACGGAGCTGTTCAAGGTGAAGAACGCCTACGAGGAGT CTCTGGACCACCTGGAGACGCTGAGGAGAGAGAACAAGAATCTGCAACAGGAGATCTCTGACCTGACTGA GCAGATTGCAGAGGGAGGGAAGCACATCCATGAGCTGGAGAAAATCAAGAAGCAAGTGGAACAGGAGAAG TGTGAGATACAGGCTGCCTTAGAGGAAGCAGAGGCATCTCTGGAGCACGAGGAGGGAAAGATCCTGCGCA TCCAGCTGGAGCTGAACCAAGTCAAGTCTGAGATCGACAGGAAGATTGCTGAGAAGGATGAGGAGATCGA CCAGCTGAAGAGGAACCACGTTAGAGTCGTGGAGACCATGCAGAGCACGCTGGATGCCGAGATCAGGAGC AGGAATGATGCTCTGAGAGTCAAGAAGAAGATGGAGGGAGACCTGAACGAGATGGAGATCCAGCTGAACC ACGCCAACCGCTTGGCTGCTGAAAGTTTGAGGAACTACAGGAACACACAGGGCATCCTCAAGGACACCCA GCTGCATCTGGATGACGCTCTCCGGGGCCAGGAGGATCTGAAGGAGCAGCTGGCCATCGTGGAGCGCAGA GCCAACCTGCTGCAGGCTGAGATTGAGGAGCTGCGGGCCACGCTGGAGCAGACGGAGAGGAGCAGGAAGA TTGCAGAGCAGGAGCTGCTGGACGCCAGTGAGCGCGTGCAGCTCCTCCACACCCAGAACACGAGCCTCAT CAACACCAAGAAGAAGCTGGAAAATGATGTTTCACAGCTGCAGAGTGAAGTGGAAGAAGTGATTCAAGAG TCACGCAATGCAGAAGAGAAGGCTAAGAAAGCCATTACTGATGCCGCCATGATGGCGGAGGAGCTGAAGA AGGAGCAGGACACCAGCGCCCACCTGGAGCGGATGAAGAAGAACATGGAACAGACGGTGAAGGACCTGCA GCACCGTCTGGACGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATCCAGAAACTGGAGGCC AGGGTACGTGAACTTGAAGGAGAGGTCGAAAATGAACAGAAACGCAATGCTGAGGCTGTTAAAGGGTTAA GGAAGCATGAAAGAAGAGTAAAGGAACTTACCTACCAGACTGAGGAAGACCGCAAGAATGTGCTCCGGCT GCAGGACCTGGTGGACAAATTACAGGCGAAGGTGAAATCCTACAAGAGACAGGCTGAGGAGGCTGAGGAA CAATCCAATGCCAACCTGGCCAAGTTCCGCAAGCTGCAGCACGAGCTGGAGGAAGCCGAGGAGCGGGCTG ACATCGCCGAGTCCCAGGTCAACAAGCTGCGGGTGAAGAGCCGGGAGGTTCACACCAAAATCAGCGCAGA G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR220412 representing NM_177369 Red=Cloning site Green=Tags(s) MSAGSDAEMAIFGEAAPYLRKSEKERIEAQNKPFDAKTSVFVAEPKESYVKSVIQSKDGGKVTVKTESGA TLTVKEDQVFPMNPPKYDKIEDMAMMTHLHEPGVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPE VVAAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGDKKKE ESGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQL KAERSYHIFYQITSNKKPELIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIDILGFSPEEKV SIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQCLNSADLLKALCYPRVKVGNEYVTKGQTV QQVYNAVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQ FFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPLGIFSILEEECMFPKATDTSFKNKLYDQHL GKSNNFQKPKPTKGKAEAHFSLVHYAGTVDYNITGWLDKNKDPLNDTVVGLYQKSAMKTLASLFSTYASA EADGGAKKGAKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNG VLEGIRICRKGFPSRILYGDFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKVFFKA GLLGLLEEMRDEKLAQIITRTQAVCRGYLMRVEYQKMLLRRESIFCIQYNVRAFMNVKHWPWMKLFFKIK PLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKMVTLLKEKNDLQLQVQSEADSLADAEERCEQ LIKNKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKN LTEEMAGLDENIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMD LERAKRKLEGDLKLAQESTMDIENDKQQLDEKLKKKEFEISNLISKIEDEQAVEIQLQKKIKELQARIEE LEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQVEMNKKRETEFQKLRRDLEEATLQHEA TAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSELKMEIDDLSSNAEAIAKAKGNLEKMCRTLEDQVS ELKSKEEEQQRLINELTAQRARLQTEAGEYSRQLDEKDALVSQLSRSKQASTQQIEELKRQLEEETKAKN ALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRL QAAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERTNAACAALDKKQRNFDKVLSEWRQKYEETQAEL ESCQKESRTLSTELFKVKNAYEESLDHLETLRRENKNLQQEISDLTEQIAEGGKHIHELEKIKKQVEQEK CEIQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHVRVVETMQSTLDAEIRS RNDALRVKKKMEGDLNEMEIQLNHANRLAAESLRNYRNTQGILKDTQLHLDDALRGQEDLKEQLAIVERR ANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLENDVSQLQSEVEEVIQE SRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQHRLDEAEQLALKGGKKQIQKLEA RVRELEGEVENEQKRNAEAVKGLRKHERRVKELTYQTEEDRKNVLRLQDLVDKLQAKVKSYKRQAEEAEE QSNANLAKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKISAE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_177369
ORF Size	5811 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_177369.3, NP_796343.2
RefSeq Size	6149 bp
RefSeq ORF	5814 bp
Locus ID	17885
UniProt ID	P13542
MW	222.7 kDa
Gene Summary	This gene encodes a myosin heavy chain. The encoded protein forms a hexamer with two heavy chains, two alkali light chains, and two regulatory light chain components. This complex functions in muscle contraction. This gene is located in a cluster of related genes on chromosome 11. [provided by RefSeq, Jun 2013]

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