Tmem175 (NM_028223) Mouse Tagged ORF Clone
CAT#: MR213249
- TrueORF®
Tmem175 (Myc-DDK-tagged) - Mouse transmembrane protein 175 (Tmem175), transcript variant 1
ORF Plasmid: tGFP
"NM_028223" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 3010001K23Rik; AI504381; mTMEM175 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR213249 representing NM_028223
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGGCTCCAGACTGAGGAGCAGGCAGTGGATTCCGAGGGAGACTCATCCCTACACAGGAGAAATG AGGAGGGGACACAGAGCTCCCACCGCATGCTGGGCTTCAGTGATGCACTGCTGTCCATCATCGCCACTGT CATGATCCTGCCTGTGACCCACACAGAGATCTCACCAGAACAGCAGTTTGACAAAAGTATACAGAAACTC CTAGCAACTAGGATCGCTGTCTACCTCATGACATTTCTAATCGTAACTGTGGCCTGGACAGCACATACCA GATTGTTCCAGGTTGTTGGAAAAATAGATGATACACTTGCCTTGCTCAACCTGGCCTGTATGATGACTAT CACCCTCCTGCCCTACACATTTTCACTAATGGTAACATTCCCTGATGTGCCCCTGGGCATCTTCCTGTTC TGCGTGTGTGTGATCGCCATTGGATCTGTGCAGGCAATGATTGTGGGCTATGCCTTCCACTTCCCACATC TGCTGAATCCACAAATCCAGTGCTCTACACATAGGGATCTATCCCGGCGGCATATCCTGCACCTGGTCCT CCGTGGCCCAGCACTGTGTTTTGTTGCGGCTGTCTTTTCCCTCTTCTTCTTCCCCTTGTCATACCTGCTG ATGGTGACTGTCATCTTCCTCCCTCACATCAGCAAGGCTACCACCTGGTGCAAAGACAAGTTGATGGGCC AAAGGGAGTCTCCAGCTCACGACATGGAGCCCTTCAGTATTGACCTGCATGCGCCCCTCAGCAAAGAGCG GGTGGAAGCTTTCAGCGACGGTGTCTATGCCATCGTGGCCACACTCCTCATCCTGGACATCTGTGAGGAC AATGTTCCGGATCCCAAGGATGTGCAAGAGAAATTCAGTGGCAGCCTTGTGGCTGCCCTGGGTGCATATG GGCCACAATTCTTGGCATACTTCGGCTCCTTCGCCACAGTGGGTCTCCTCTGGTTTGCTCACCATTCACT CTTCCTGCATGTCCGGAAGGCTACACAGACCATGGGGTTACTCAACATACTATCGCTGGCCTTTGTGGGT GGCCTTCCTCTGGCCTACCAGCAGACCTCAGCTTTTGCCCGGCAACCCCATGATGAACTTGAGCGTGTGC GTGTCAGCTGTGCCATCATCTTCTTTGCCAGCATCTTCCAGTTTGCCATCTGGACTACAGCCCTGCTGCA TCAGACAGAAACACTGCAGCCAGCTGTGCAATTTGGTGGGCAGGAGCATGCTTTCATGTTTGCCAAGCTT GCACTGTATCCCTGTGCCAGCCTGCTGGCCTTTGCTGCCACCTGCCTACTGAGTCGTTTCAGCACAGCCA TCTTCCACCTCATGCAGATTTCAGTGCCCTTTGCATTCCTGTTGCTCCGTCTCCTTGTACGCCTTGCCCT GGCAGGCCTTCAAGTCCTACGGGGCCTCTGGCCACACCATCCCCAACAAGACCAGAGTGAACCTGAGGCA CAGTCCCAGCTCTTGCCTGACCCCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR213249 representing NM_028223
Red=Cloning site Green=Tags(s) MSRLQTEEQAVDSEGDSSLHRRNEEGTQSSHRMLGFSDALLSIIATVMILPVTHTEISPEQQFDKSIQKL LATRIAVYLMTFLIVTVAWTAHTRLFQVVGKIDDTLALLNLACMMTITLLPYTFSLMVTFPDVPLGIFLF CVCVIAIGSVQAMIVGYAFHFPHLLNPQIQCSTHRDLSRRHILHLVLRGPALCFVAAVFSLFFFPLSYLL MVTVIFLPHISKATTWCKDKLMGQRESPAHDMEPFSIDLHAPLSKERVEAFSDGVYAIVATLLILDICED NVPDPKDVQEKFSGSLVAALGAYGPQFLAYFGSFATVGLLWFAHHSLFLHVRKATQTMGLLNILSLAFVG GLPLAYQQTSAFARQPHDELERVRVSCAIIFFASIFQFAIWTTALLHQTETLQPAVQFGGQEHAFMFAKL ALYPCASLLAFAATCLLSRFSTAIFHLMQISVPFAFLLLRLLVRLALAGLQVLRGLWPHHPQQDQSEPEA QSQLLPDPC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_028223 |
ORF Size | 1497 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028223.3, NP_082499.3 |
RefSeq Size | 3073 bp |
RefSeq ORF | 1500 bp |
Locus ID | 72392 |
UniProt ID | Q9CXY1 |
MW | 56 kDa |
Gene Summary | Organelle-specific potassium channel specifically responsible for potassium conductance in endosomes and lysosomes. Forms a potassium-permeable leak-like channel, which regulates lumenal pH stability and is required for autophagosome-lysosome fusion. Constitutes the major lysosomal potassium channel.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217019 | Tmem175 (untagged) - Mouse transmembrane protein 175 (Tmem175), transcript variant 1, (10ug) |
CNY 6,460.00 |
|
MG213249 | Tmem175 (tGFP-tagged) - Mouse transmembrane protein 175 (Tmem175) transcript variant 1, (10ug) |
CNY 3,710.00 |
|
MR213249L3 | Lenti ORF clone of Tmem175 (Myc-DDK-tagged) - Mouse transmembrane protein 175 (Tmem175), transcript variant 1 |
CNY 5,230.00 |
|
MR213249L4 | Lenti ORF clone of Tmem175 (mGFP-tagged) - Mouse transmembrane protein 175 (Tmem175), transcript variant 1 |
CNY 5,230.00 |
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