Pitpnm2 (BC070452) Mouse Tagged ORF Clone

CAT#: MR211631

  • TrueORF®

Pitpnm2 (Myc-DDK-tagged) - Mouse phosphatidylinositol transfer protein, membrane-associated 2 (cDNA clone MGC:99420 IMAGE:30621943)

ORF Plasmid: DDK tGFP



  "BC070452" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 7,648.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms NIR3, RDGBA2, mKIAA1457
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211631 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCATAAAGGAATACCGGATTCCCCTGCCAATGACCGTGGATGAATACCGCATTGCCCAGCTGTACA
TGATACAGAAGAAGAGCCGGAATGAGACCCACGGGCAAGGCAGCGGGGTAGAGATCCTGGAGAACCGGCC
CTACACAGACGGCCCTGGCGGCTCCGGGCAATACACCCACAAGGTGTATCATGTGGGCATGCACATTCCT
GGCTGGTTTCGCTCCATCTTGCCCAAAGCAGCCTTGCGGGTGGTGGAGGAGTCCTGGAATGCCTACCCCT
ATACCCGAACAAGGTTCACCTGCCCCTTTGTGGAGAAGTTCTCCATTGACATTGAAACCTTTTATAAGAC
AGACACTGGGGAAAACAATAATGTGTTCAACCTGTCTCCTGTGGAAAAGAGCCAGCTGATAACAGACATC
ATCGACATCGTCAAGGATCCTGTGCCCCCCAGTGAGTATAAGACAGAAGAAGACCCCAAGCTGTTCCAGT
CAGTCAAGACTTGCCGGGGACCTCTTTCTGAAAACTGGATTCAGGAGTACAAGAAGCGGCTCCTCCCCAT
CATGTGCGCCTACAAGCTCTGCAAGGTGGAGTTCCGATACTGGGGCATGCAGTCCAAGATTGAAAGGTTC
ATCCATGACACAGGTCTGCGGCGGGTGATGGTGAGGGCCCACCGGCAGGCCTGGTGCTGGCAGGACGAGT
GGTACGGACTGACCATGGAGAAAATCCGAGAGCTGGAGAGGGAGGTGCAGCTCATGCTGTCCCGCAAAAT
GGCCCAGTTTTCGGAGGAAGGCCCCTCAGAACTAAGCAAGGACAGTGCCACCAAGGACCAGGCATCTGGA
ACAACCTCTGATCCTGGCAGCAAAAACGGGGAGCCTCTGGGGCGGGGCCTGAAGAAGCAGTGGTCCACCT
CCTCCAAGTCCTCAAGGTCCTCCAAGCGGGGAGCCAGCCCCTCCCGACATAGCATCTCAGAGTGGAGGAT
GCAGAGTATCGCCAGGGACTCCGACGAAGGCTCGGAAGAGGAGTTCTTCGATGCACATGAGAACCTGTAC
TGCACAGAGGAAAAACAAGCCAAGGACATGACCAAGTGGAACTCCAACGACCTCATGGACAAAATGGAGA
GTCCGGAGCCTGAGGAATCACAAGATGAAATCTACCAGCAGAGTGGCTCTGAGTTCAGGGTGGCCTCCAG
CTTGGAGCAGCTGAACATCATCGAGGATGAGGTGAGCCAGCCACTAGCCGCGCCACCCTCCAAGATTCAC
GTGCTGCTGCTGGTGCTGCATGGAGGCACCATCCTGGACACTGGAGCGGGGGACCCCAGCTCCAAGCAGG
GGGACACCAATACTATCACCAACGTGTTCGACACCGTCATGCGCGTGCACTACCCCAGTGCCCTAGGCCA
CCTAGCCATCCGCCTGGTGCCCTGCCCACCCATCTGTGCTGATGCCTTCGCCCTCGTCTCCAACCTCAGT
CCCTATGGCCATGATGAGGGCTGTCTGTCTAGCAGCCAGGACCACATCCCCCTAGCTGCCCTGCCCCTGC
TGGCCACTTCCTCACCCCAGTACCAGGAGGCAGTTGCTACAGTGATTCAGCGGGCCAACCTGGCCTACGG
AGACTTCATCAAGTCCCAGGAAGGTGTGACCTTCAATGGTCAGGTCTGCCTGATTGGAGACTGTGTTGGG
GGCATCCTGGCATTTGATGCCTTATGCTACAGTGGCCAGCCAGTGTCTGAGAGTCAAAGCAGTAGCCTGC
CGCCTTTCAGCATCCCTCGGTACCAGCGCTACCCGCTGGGGGACGGCTGCTCCACACTGCTGGCGGATGT
GCTTCAGACCCACAACACAGTCTTCCAAGAGCATGCAGCCCCCTCGTCACCTGGCACAGCCCCTGCTGGC
CGTGGCTTCCGCAGAGCCAGTGAGATCAGTATCGCCAGTCAGGTGTCAGGCATGGCCGAGAGCTACACAG
CGTCCAGCATCGCCCAGATTGCTGCCAAGTGGTGGGGACAGAAGCGGATTGACTATGCCCTGTATTGCCC
GGACGCCCTCACGGCCTTCCCCACGGTGGCCTTGCCCCACCTCTTCCACGCCAGCTACTGGGAGTCAACC
GACGTGGTCTCCTTTCTGCTGAGACAGGTCATGCGGCACGACAGCTCGAGCATCCTAGAGCTGGACGGCA
AGGAGGTGTCCGTGTTCACGCCTTCACAGCCCAGGGAAAGGTGGCAGCGCAAGAGGACCCACGTGAAGCT
GCGGAACGTGGCAGCCAACCACCGGATCAATGACGCAGTTGCCAATGAGGATGGCCCGCAGGTTGTGACG
GGCCGGTTCATGTATGGGCCCTTAGACATGGTCACCCTGACTGGGGAAAAGGTGGATGTGCACATCATGA
CGCAACCACCTTCAGGTGAGTGGCTGCACCTAGACACTCTAGTGACCAACAGCAGTGGGCGCGTCTCCTA
CACCATTCCAGAAACACACCGCCTGGGGGTGGGCGTCTACCCCATCAAGATGGTGGTCAGGGGAGACCAC
ACATTCGCCGACAGCTACATCACTGTGCTCCCCAGGGGCACAGAGTTTGTGGTCTTCAGTATAGACGGCT
CCTTTGCCGCCAGTGTGTCCATCATGGGCAGCGACCCCAAGGTGCGCGCCGGGGCTGTGGACGTGGTGCG
ACACTGGCAGGACCTGGGCTACCTCATCATCTATGTGACCGGCCGGCCCGACATGCAAAAGCAGCGGGTG
GTGGCATGGCTGGCTCAGCACAATTTCCCTCATGGTGTGGTGTCCTTCTGTGATGGCCTGGTGCACGACC
CACTGCGCCACAAGGCTAACTTTCTGAAGCTGCTCATCTCTGAGCTTCACCTGCGCGCACATGCGGCCTA
CGGCTCCACGAAGGATGTCGCAGTCTATAACTCCATCAGCCTCTCTCCCATGCACATCTACATTGTGGGC
CGCCCCACCAAGAAGCTGCAGCAGCAGTGCCAGTTCATCACGGACGGCTATGCGGCCCACCTGGCCCAGC
TCAAGTACAATCACCGGGCTCGCCCAGCCCGGAACACGGCCACGCGCATGGCTTTGCGCAAGGGCAGCTT
CGGCCTACCTGGCCAGAGTGACTTCCTTCGCTCTCGGAATCACCTGCTCCGCACCATCTCGGCCCAGCCC
AGTGGGCCCAGCCATCGGCACGATCGGACACAGACCCAGATGGACAGCGAGCAGCGGGGCCAGCGCAGCA
TGAGCGTGGCAGCCAGCTGCTGGGGCCGAGCCATGGCAGGCCGGCTTGAACCAGGGGCAGCCACGGGACC
CAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211631 protein sequence
Red=Cloning site Green=Tags(s)

MIIKEYRIPLPMTVDEYRIAQLYMIQKKSRNETHGQGSGVEILENRPYTDGPGGSGQYTHKVYHVGMHIP
GWFRSILPKAALRVVEESWNAYPYTRTRFTCPFVEKFSIDIETFYKTDTGENNNVFNLSPVEKSQLITDI
IDIVKDPVPPSEYKTEEDPKLFQSVKTCRGPLSENWIQEYKKRLLPIMCAYKLCKVEFRYWGMQSKIERF
IHDTGLRRVMVRAHRQAWCWQDEWYGLTMEKIRELEREVQLMLSRKMAQFSEEGPSELSKDSATKDQASG
TTSDPGSKNGEPLGRGLKKQWSTSSKSSRSSKRGASPSRHSISEWRMQSIARDSDEGSEEEFFDAHENLY
CTEEKQAKDMTKWNSNDLMDKMESPEPEESQDEIYQQSGSEFRVASSLEQLNIIEDEVSQPLAAPPSKIH
VLLLVLHGGTILDTGAGDPSSKQGDTNTITNVFDTVMRVHYPSALGHLAIRLVPCPPICADAFALVSNLS
PYGHDEGCLSSSQDHIPLAALPLLATSSPQYQEAVATVIQRANLAYGDFIKSQEGVTFNGQVCLIGDCVG
GILAFDALCYSGQPVSESQSSSLPPFSIPRYQRYPLGDGCSTLLADVLQTHNTVFQEHAAPSSPGTAPAG
RGFRRASEISIASQVSGMAESYTASSIAQIAAKWWGQKRIDYALYCPDALTAFPTVALPHLFHASYWEST
DVVSFLLRQVMRHDSSSILELDGKEVSVFTPSQPRERWQRKRTHVKLRNVAANHRINDAVANEDGPQVVT
GRFMYGPLDMVTLTGEKVDVHIMTQPPSGEWLHLDTLVTNSSGRVSYTIPETHRLGVGVYPIKMVVRGDH
TFADSYITVLPRGTEFVVFSIDGSFAASVSIMGSDPKVRAGAVDVVRHWQDLGYLIIYVTGRPDMQKQRV
VAWLAQHNFPHGVVSFCDGLVHDPLRHKANFLKLLISELHLRAHAAYGSTKDVAVYNSISLSPMHIYIVG
RPTKKLQQQCQFITDGYAAHLAQLKYNHRARPARNTATRMALRKGSFGLPGQSDFLRSRNHLLRTISAQP
SGPSHRHDRTQTQMDSEQRGQRSMSVAASCWGRAMAGRLEPGAATGPK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC070452
ORF Size 3294 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC070452, AAH70452
RefSeq Size 6235 bp
RefSeq ORF 3296 bp
Locus ID 19679
MW 122.6 kDa
Gene Summary Catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes (in vitro). Binds calcium ions (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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