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Vps53 (BC034371) Mouse Tagged ORF Clone
CAT＃: MR209806
- TrueORF®
Vps53 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 53 (yeast) (cDNA clone MGC:19253 IMAGE:3964008)
ORF Plasmid: tGFP
"BC034371" in other vectors (4)
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CNY 4,880.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RP23-147P4.2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209806 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGAGGAGGAGGAACTGGAGTTTGTGGAGGAGCTGGAGGCTGTGCTGCAGCTCACGCCAGAGGTGC AACTCGCCATCGAGCAGGTATTCCCAAGCCAGGATCCTCTTGATCAAGCTGACTTCAATGCCGTTGAGTA TATCAATACTCTGTTCCCAACAGAGCAATCTCTGGCAAACATTGACGATGTTGTGAACAAAATTAGACTG AAAATAAGGAGACTGGATGACAATATCCGGACTGTCGTAAGAGGTCAGACAAATGTGGGGCAGGATGGAA GGCAAGCACTTGAAGAGGCCCAGAAAGCCATTCAGCAACTCTTTGGCAAAATCAAGGATATCAAAGACAA AGCAGAAAAGTCAGAACAAATGGTGAAAGAGATCACCCGAGATATTAAGCAGTTAGATCACGCCAAGCGC CACCTGACCACCTCAATCACGACCCTGAACCACCTGCACATGCTGGCAGGCGGGGTGGACTCCCTGGAAG CCATGACCAGGCGCAGACAGTATGGAGAAGTTGCTAATCTCCTTCAGGGTGTAATGAATGTTCTGGAGCA CTTTCACAAATACATGGGAATCCCACAGATCCGACAGCTTTCTGAAAGGGTGAAGGCTGCACAGACAGAG TTAGGACAGCAAATCCTGGCTGACTTTGAAGAAGCGTTTCCTTCGCAGGGTACCAAGCCGAAGAAGCCCA AGGCCCCGGACAACCCGTTCCACGGCATCGTTTCCAAGTGTTTTGAGCCTCATCTCTACGTGTACATTGA GTCCCAGGACAAAAACCTCAGCGAGCTGATAGACCGGTTTGTGGCAGATTTCAAAGCCCAGGGCCCACCC AAGCCCAACACTGACGAAGGGGGTGCTGTGCTCCCCAGCTGCGCTGACCTCTTTGTCTACTACAAGAAGT GCATGGTGCAGTGCTCACAGCTCAGCACAGGCGAGCCCATGATCGCCCTGACTACCATCTTCCAGAAGTA CCTCCGAGAATACGCCTGGAAAATCCTCTCTGGCAACCTGCCTAAAACCAGCAGCAGCAGTGGGGGGCTG ACCATCAGCAGCCTCCTCAAAGAGAAGGAGGGCTCAGAAGTGGCCAGGTTCACTCTGGAGGAGCTGTGTC TCATCTGCAGCATCCTGAGCACAGCCGAGTATTGTCTGGCTACCACCCAGCAGCTAGAAGAAAAACTCAA AGAAAAAGTTGATGTGAGCCTGACTGAACGAATTAATCTCACAGGAGAAATGGACACATTCAGCACTGTC ATCTCCAGCAGCATCCAGCTGTTGGTTCAAGATCTGGATGCAGCCTGTGACCCTGCCCTGATTGCCATGA GCAAGATGCCTTGGCAGAACGTGGAGCATGTTGGTGACCAGAGCCCTTATGTCACTTCTGTTATTCTTCA CATTAAGCAAAACGTCCCCATCATCCGTGACAACCTGGCTTCCACACGGAAATACTTCACACAGTTCTGC ATTAAATTTGCAAATTCGTTCATTCCCAAATTCATCACCCACCTCTTCAAGTGTAAGCCGATCAGCATGG TGGGAGCAGAGCAGCTTCTGCTGGACACCCACTCCCTGAAGATGGTCCTGCTCGACCTTCCGTCCATTGG CTCCCAGGTGGTGAGGAAGGCCCCTGCCAGTTACACTAAGATTGTCGTGAAAGGCATGACCCGGGCTGAA ATGATCCTTAAGGTGGTGATGGCCCCTCATGAACCGTTGGTGGTATTTGTTGACAACTACATCAAACTCC TTACGGACTGCAACTCAGAAACCTTCCAGAAAATACTGGATATGAAGGGGCTGAAGAGGAGTGAGCAGAG CAGCATGCTGGAACTTTTGCGGCAGAGGCTCCCTGCCCCGCCCTCGGGGACAGAAGGTTCCAGCACCCTG TCGCTGATTGCCCCAACACCGGAGCAGGAGTCATCCCGAATCCGCAAACTGGAGAAACTGATTAAAAAGA GACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209806 protein sequence
Red=Cloning site Green=Tags(s) MMEEEELEFVEELEAVLQLTPEVQLAIEQVFPSQDPLDQADFNAVEYINTLFPTEQSLANIDDVVNKIRL KIRRLDDNIRTVVRGQTNVGQDGRQALEEAQKAIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKR HLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTE LGQQILADFEEAFPSQGTKPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLSELIDRFVADFKAQGPP KPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTSSSSGGL TISSLLKEKEGSEVARFTLEELCLICSILSTAEYCLATTQQLEEKLKEKVDVSLTERINLTGEMDTFSTV ISSSIQLLVQDLDAACDPALIAMSKMPWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFC IKFANSFIPKFITHLFKCKPISMVGAEQLLLDTHSLKMVLLDLPSIGSQVVRKAPASYTKIVVKGMTRAE MILKVVMAPHEPLVVFVDNYIKLLTDCNSETFQKILDMKGLKRSEQSSMLELLRQRLPAPPSGTEGSSTL SLIAPTPEQESSRIRKLEKLIKKRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC034371 |
| ORF Size | 1965 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC034371, AAH34371 |
| RefSeq Size | 2225 bp |
| RefSeq ORF | 1967 bp |
| Locus ID | 68299 |
| MW | 73.7 kDa |
| Gene Summary | Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218399 | Vps53 (untagged) - Mouse vacuolar protein sorting 53 (yeast) (cDNA clone MGC:19253 IMAGE:3964008), (10ug) |
CNY 6,940.00 |
|
| MG209806 | Vps53 (tGFP-tagged) - Mouse vacuolar protein sorting 53 (yeast) (cDNA clone MGC:19253 IMAGE:3964008) |
CNY 4,090.00 |
|
| MR209806L3 | Lenti ORF clone of Vps53 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 53 (yeast) (cDNA clone MGC:19253 IMAGE:3964008) |
CNY 5,610.00 |
|
| MR209806L4 | Lenti ORF clone of Vps53 (mGFP-tagged) - Mouse vacuolar protein sorting 53 (yeast) (cDNA clone MGC:19253 IMAGE:3964008) |
CNY 5,610.00 |
