Trim25 (BC006908) Mouse Tagged ORF Clone

CAT#: MR209551

  • TrueORF®

Trim25 (Myc-DDK-tagged) - Mouse tripartite motif protein 25 (cDNA clone MGC:6886 IMAGE:2651664)

ORF Plasmid: DDK tGFP



  "BC006908" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 4,664.00


货期*
现货

规格
    • 10 ug

Cited in 2 publications.

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AA960166; AL022677; EFP; Zfp147
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR209551 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGCTGAATCCTCTGGCCGAGGAGCTGTCCTGCTCCGTCTGCCTGGAGCTCTTCAAGGAACCTG
TCACCACCCCGTGCGGCCACAACTTCTGCATGTCATGCCTGGATGAGACGTGGGTCGTCCAGGGCCCGCC
GTACCGGTGCCCGCAGTGTCGCAAAGTGTACCAGGTGCGCCCGCAGCTGCAAAAGAACACGGTAATGTGC
GCGGTGGTGGAGCAGTTCCTGCAGGCTGAGCAGGCGCGGACCCCGGTGAACGACTGGACGCCCCCTGCCC
GCTCCTCCGCTTCCAGCGCAGCCACCCAGGTGGCCTGCGACCACTGCCTGACGGAGATCGCAGTGAAGAC
GTGCCTCGTGTGCATGGCCTCTTTCTGCCAGGAGCACTTGAGGCCTCACTTCGACAGTCCGGCCTTCCAG
GATCACCCCCTGCAGTCGCCCATCCGTGACCTTCTGCGCCGCAAATGTACCCAGCACAACCGCCTTCGGG
AATTGTTTTGTCCCGAGCATGGCGAGTGCATCTGCCATATCTGCTTGGTAGAGCACAAGACCTGCTCCCC
TACGACCCTAAGTCAAGCCAGCGCCGACCTGGAGTACAAACTGAGGAATAAACTCACTATCATGCACAGC
CACATCAATGGGGCAACCAAAGCATTGGAGGATGTGAGATCCAAGCAACAGTGTGTGCAGGATTCTATGA
AGAGGAAGATGGAACAGCTGAGACAGGAGTATATGGAAATGAAGGCTGTCATCGACGCAGCAGAGACCAG
CTCCTTGCGCAGGTTGAAGGAGGAGGAGAAGAGAGTCTACGGAAAATTCGACACCATCTACCAGGTTCTC
GTCAAGAAGAAGAGTGAGATGCAGAAACTGAAGGCAGAGGTTGAGCTCATTATGGACAAAGGGGATGAGT
TTGAGTTTCTGGAGAAAGCTGCAAAATTGCAAGGAGAGTCAACAAAACCAGTCTACATCCCTAAGATAGA
TCTGGATCATGATTTGATAATGGGAATCTACCAAGGGGCGGCGGACCTCAAGAGTGAACTGAAGCACTCC
ATTAAGAAGCTACAGAAGAAGTCTGAGGAGCACAATGGCTCAGGTAACAAGGGAGACCAGACACAGTCCA
CTTTTAAACCCGTACAACCTTCGAAGAAGACCATTCCTGTTGCTCCCGGCTCTCCCTCCCACTTCTCACC
TAACAAGTTGCCCACCTTTGGAGCTCCTGGCCAATCACTGGATTCAAAAGCAACTTCCCCTGATGCCGCA
CCCAAAGCCAGTGCTGCACAGCCAGACTCTGTAGGAGTCAAGGCTAAGGTGCTAGAAAACTTCTTAACCA
AGTCCAGAACGGAGCTTCTGGAGTATTTCGTGAAAGTCATCTTCGACTACAATACCGCCCACAACAAAGT
GTCTCTGTCAAACAAGTACACCACCGCCTCTGTGTCTGATGGGCTCCAGCACTACCGGTCCCATCCCCAG
AGGTTCACCTACTGCTCTCAGGTCCTGGGGCTGCACTGCTACAAGAACGGCATTCACTACTGGGAGGTGG
AACTACAGAAGAACAACTTCTGCGGCGTAGGCATCTGCTACGGCAGCATGGAACGGCAGGGCCCCGAGAG
CCGGCTGGGCCGCAACCCCAACTCATGGTGTGTGGAGTGGTTCAATAACAAGATCTCCGCCTGGCACAAC
AACGTGGAGAAGACTCTGCCCTCCACCAAGGCCACTCGAGTCGGCGTGCTGCTCAACTGTGACCACGGCT
TCGTCATTTTCTTTGCGGTCACCGAAAAGGTGCACCTGATGTACAAGTTCAAGGTGGACTTCACCGAAGC
TCTGTACCCAGCCTTCTGGGTGTTCTCGGCCGGTACCACCCTCTCTATCTGCTCCAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR209551 protein sequence
Red=Cloning site Green=Tags(s)

MAELNPLAEELSCSVCLELFKEPVTTPCGHNFCMSCLDETWVVQGPPYRCPQCRKVYQVRPQLQKNTVMC
AVVEQFLQAEQARTPVNDWTPPARSSASSAATQVACDHCLTEIAVKTCLVCMASFCQEHLRPHFDSPAFQ
DHPLQSPIRDLLRRKCTQHNRLRELFCPEHGECICHICLVEHKTCSPTTLSQASADLEYKLRNKLTIMHS
HINGATKALEDVRSKQQCVQDSMKRKMEQLRQEYMEMKAVIDAAETSSLRRLKEEEKRVYGKFDTIYQVL
VKKKSEMQKLKAEVELIMDKGDEFEFLEKAAKLQGESTKPVYIPKIDLDHDLIMGIYQGAADLKSELKHS
IKKLQKKSEEHNGSGNKGDQTQSTFKPVQPSKKTIPVAPGSPSHFSPNKLPTFGAPGQSLDSKATSPDAA
PKASAAQPDSVGVKAKVLENFLTKSRTELLEYFVKVIFDYNTAHNKVSLSNKYTTASVSDGLQHYRSHPQ
RFTYCSQVLGLHCYKNGIHYWEVELQKNNFCGVGICYGSMERQGPESRLGRNPNSWCVEWFNNKISAWHN
NVEKTLPSTKATRVGVLLNCDHGFVIFFAVTEKVHLMYKFKVDFTEALYPAFWVFSAGTTLSICSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC006908
ORF Size 1878 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC006908, AAH06908
RefSeq Size 2602 bp
RefSeq ORF 1880 bp
Locus ID 217069
MW 70.7 kDa
Gene Summary Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58 and IFIH1. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Mediates 'Lys-63'-linked polyubiquitination of IFIH1. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (2)

Other Versions

Customer Reviews 
Loading...