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Trim25 (BC006908) Mouse Tagged ORF Clone
CAT＃: MR209551
- TrueORF®
Trim25 (Myc-DDK-tagged) - Mouse tripartite motif protein 25 (cDNA clone MGC:6886 IMAGE:2651664)
ORF Plasmid: tGFP
"BC006908" in other vectors (4)
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CNY 4,664.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA960166; AL022677; EFP; Zfp147 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209551 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCTGAATCCTCTGGCCGAGGAGCTGTCCTGCTCCGTCTGCCTGGAGCTCTTCAAGGAACCTG TCACCACCCCGTGCGGCCACAACTTCTGCATGTCATGCCTGGATGAGACGTGGGTCGTCCAGGGCCCGCC GTACCGGTGCCCGCAGTGTCGCAAAGTGTACCAGGTGCGCCCGCAGCTGCAAAAGAACACGGTAATGTGC GCGGTGGTGGAGCAGTTCCTGCAGGCTGAGCAGGCGCGGACCCCGGTGAACGACTGGACGCCCCCTGCCC GCTCCTCCGCTTCCAGCGCAGCCACCCAGGTGGCCTGCGACCACTGCCTGACGGAGATCGCAGTGAAGAC GTGCCTCGTGTGCATGGCCTCTTTCTGCCAGGAGCACTTGAGGCCTCACTTCGACAGTCCGGCCTTCCAG GATCACCCCCTGCAGTCGCCCATCCGTGACCTTCTGCGCCGCAAATGTACCCAGCACAACCGCCTTCGGG AATTGTTTTGTCCCGAGCATGGCGAGTGCATCTGCCATATCTGCTTGGTAGAGCACAAGACCTGCTCCCC TACGACCCTAAGTCAAGCCAGCGCCGACCTGGAGTACAAACTGAGGAATAAACTCACTATCATGCACAGC CACATCAATGGGGCAACCAAAGCATTGGAGGATGTGAGATCCAAGCAACAGTGTGTGCAGGATTCTATGA AGAGGAAGATGGAACAGCTGAGACAGGAGTATATGGAAATGAAGGCTGTCATCGACGCAGCAGAGACCAG CTCCTTGCGCAGGTTGAAGGAGGAGGAGAAGAGAGTCTACGGAAAATTCGACACCATCTACCAGGTTCTC GTCAAGAAGAAGAGTGAGATGCAGAAACTGAAGGCAGAGGTTGAGCTCATTATGGACAAAGGGGATGAGT TTGAGTTTCTGGAGAAAGCTGCAAAATTGCAAGGAGAGTCAACAAAACCAGTCTACATCCCTAAGATAGA TCTGGATCATGATTTGATAATGGGAATCTACCAAGGGGCGGCGGACCTCAAGAGTGAACTGAAGCACTCC ATTAAGAAGCTACAGAAGAAGTCTGAGGAGCACAATGGCTCAGGTAACAAGGGAGACCAGACACAGTCCA CTTTTAAACCCGTACAACCTTCGAAGAAGACCATTCCTGTTGCTCCCGGCTCTCCCTCCCACTTCTCACC TAACAAGTTGCCCACCTTTGGAGCTCCTGGCCAATCACTGGATTCAAAAGCAACTTCCCCTGATGCCGCA CCCAAAGCCAGTGCTGCACAGCCAGACTCTGTAGGAGTCAAGGCTAAGGTGCTAGAAAACTTCTTAACCA AGTCCAGAACGGAGCTTCTGGAGTATTTCGTGAAAGTCATCTTCGACTACAATACCGCCCACAACAAAGT GTCTCTGTCAAACAAGTACACCACCGCCTCTGTGTCTGATGGGCTCCAGCACTACCGGTCCCATCCCCAG AGGTTCACCTACTGCTCTCAGGTCCTGGGGCTGCACTGCTACAAGAACGGCATTCACTACTGGGAGGTGG AACTACAGAAGAACAACTTCTGCGGCGTAGGCATCTGCTACGGCAGCATGGAACGGCAGGGCCCCGAGAG CCGGCTGGGCCGCAACCCCAACTCATGGTGTGTGGAGTGGTTCAATAACAAGATCTCCGCCTGGCACAAC AACGTGGAGAAGACTCTGCCCTCCACCAAGGCCACTCGAGTCGGCGTGCTGCTCAACTGTGACCACGGCT TCGTCATTTTCTTTGCGGTCACCGAAAAGGTGCACCTGATGTACAAGTTCAAGGTGGACTTCACCGAAGC TCTGTACCCAGCCTTCTGGGTGTTCTCGGCCGGTACCACCCTCTCTATCTGCTCCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209551 protein sequence
Red=Cloning site Green=Tags(s) MAELNPLAEELSCSVCLELFKEPVTTPCGHNFCMSCLDETWVVQGPPYRCPQCRKVYQVRPQLQKNTVMC AVVEQFLQAEQARTPVNDWTPPARSSASSAATQVACDHCLTEIAVKTCLVCMASFCQEHLRPHFDSPAFQ DHPLQSPIRDLLRRKCTQHNRLRELFCPEHGECICHICLVEHKTCSPTTLSQASADLEYKLRNKLTIMHS HINGATKALEDVRSKQQCVQDSMKRKMEQLRQEYMEMKAVIDAAETSSLRRLKEEEKRVYGKFDTIYQVL VKKKSEMQKLKAEVELIMDKGDEFEFLEKAAKLQGESTKPVYIPKIDLDHDLIMGIYQGAADLKSELKHS IKKLQKKSEEHNGSGNKGDQTQSTFKPVQPSKKTIPVAPGSPSHFSPNKLPTFGAPGQSLDSKATSPDAA PKASAAQPDSVGVKAKVLENFLTKSRTELLEYFVKVIFDYNTAHNKVSLSNKYTTASVSDGLQHYRSHPQ RFTYCSQVLGLHCYKNGIHYWEVELQKNNFCGVGICYGSMERQGPESRLGRNPNSWCVEWFNNKISAWHN NVEKTLPSTKATRVGVLLNCDHGFVIFFAVTEKVHLMYKFKVDFTEALYPAFWVFSAGTTLSICSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC006908 |
| ORF Size | 1878 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC006908, AAH06908 |
| RefSeq Size | 2602 bp |
| RefSeq ORF | 1880 bp |
| Locus ID | 217069 |
| MW | 70.7 kDa |
| Gene Summary | Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58 and IFIH1. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Mediates 'Lys-63'-linked polyubiquitination of IFIH1. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3.[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Sendai virus V protein decreases nitric oxide production by inhibiting RIG-I signaling in infected RAW264.7 macrophages
,Morita, N;Tanaka, Y;Odkhuu, E;Naiki, Y;Komatsu, T;Koide, N;,
Microbes Infect.
,PubMed ID 32032681
[TRIM25]
|
|
The Ubiquitin Ligase RNF125 Targets Innate Immune Adaptor Protein TRIM14 for Ubiquitination and Degradation
,Jia, X;Zhou, H;Wu, C;Wu, Q;Ma, S;Wei, C;Cao, Y;Song, J;Zhong, H;Zhou, Z;Wang, J;,
J. Immunol.
,PubMed ID 28476934
[TRIM25]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218581 | Trim25 (untagged) - Mouse tripartite motif protein 25 (cDNA clone MGC:6886 IMAGE:2651664), (10ug) |
CNY 6,940.00 |
|
| MG209551 | Trim25 (tGFP-tagged) - Mouse tripartite motif protein 25 (cDNA clone MGC:6886 IMAGE:2651664) |
CNY 3,990.00 |
|
| MR209551L3 | Lenti ORF clone of Trim25 (Myc-DDK-tagged) - Mouse tripartite motif protein 25 (cDNA clone MGC:6886 IMAGE:2651664) |
CNY 5,510.00 |
|
| MR209551L4 | Lenti ORF clone of Trim25 (mGFP-tagged) - Mouse tripartite motif protein 25 (cDNA clone MGC:6886 IMAGE:2651664) |
CNY 5,510.00 |
