Plk1 (NM_011121) Mouse Tagged ORF Clone
CAT#: MR209333
- TrueORF®
Plk1 (Myc-DDK-tagged) - Mouse polo-like kinase 1 (Drosophila) (Plk1)
ORF Plasmid: tGFP
"NM_011121" in other vectors (4)
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CNY 6,744.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | P; Plk; STPK; STPK13 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR209333 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGCAGCGGCCAAAGCTGGAAAGCTGGCTCGAGCACCAGCCGACCTCGGGAAAGGTGGGGTTCCGG GAGATGCAGTTCCCGGTGCCCCAGTGGCCGCCCCGCTGGCGAAAGAAATTCCGGAGGTCCTAGTGGACCC ACGCAGCCGGCGGCAGTATGTACGGGGGCGCTTTCTGGGTAAAGGAGGCTTCGCCAAATGCTTCGAGATC TCAGACGCAGACACAAAAGAGGTGTTCGCAGGCAAGATCGTGCCTAAGTCTTTGCTGCTCAAGCCCCACC AGAAGGAGAAGATGTCTATGGAGATCTCAATTCACCGCAGCCTAGCACACCAACACGTCGTAGGCTTCCA TGACTTTTTTGAGGACAGCGACTTTGTATTTGTAGTTTTGGAGCTCTGTCGCAGGAGGTCCCTCCTGGAG CTGCACAAGAGGAGGAAGGCACTGACCGAGCCTGAGGCCCGCTACTACCTGCGACAGATAGTCCTGGGCT GCCAGTACCTGCACCGCAATCAGGTCATTCACAGGGACCTCAAGCTGGGCAACCTCTTCCTGAACGAGGA TCTGGAGGTGAAAATAGGGGATTTTGGCTTGGCAACCAAAGTGGAATATGAAGGGGAACGAAAGAAGACC TTGTGTGGCACTCCTAACTACATAGCTCCTGAGGTGCTGAGCAAGAAGGGACACAGTTTTGAGGTGGATG TGTGGTCCATTGGGTGCATCATGTATACCTTGCTAGTGGGCAAGCCTCCCTTTGAGACCTCGTGCCTAAA AGAGACCTACCTCCGGATCAAGAAAAATGAATACAGTATTCCCAAGCACATCAACCCAGTGGCCGCCTCC CTCATCCAGAAGATGCTTCAGACAGACCCCACTGCCCGCCCCACCATTCACGAGTTGCTCAATGACGAGT TCTTCACTTCTGGCTACATCCCCGCCCGTCTCCCTATTACCTGCCTCACCATCCCACCAAGGTTTTCAAT CGCTCCCAGCAGCCTGGACCCCAGCAGCAGGAAACCTCTCAAAGTCCTCAATAAAGGTGTGGAGAACCCC CTGCCTGACCGTCCCCGGGAGAAAGAGGAACCGGTGGTCCGGGAGACAAATGAGGCCATTGAGTGCCACC TTAGTGACTTGCTACAGCAGCTGACCAGTGTCAACGCCTCCAAGCCCTCGGAGCGCGGGCTGGTGCGGCA AGAGGAGGCTGAGGATCCTGCCTGCATCCCCATCTTCTGGGTCAGCAAGTGGGTGGACTATTCGGACAAG TATGGCCTTGGGTATCAGCTGTGTGACAACAGTGTGGGGGTGCTTTTTAATGACTCAACACGCCTGATTC TCTACAATGACGGGGACAGCCTGCAGTACATAGAGCGTGATGGCACGGAGTCCTATCTCACTGTGAGCTC CCATCCCAATTCCTTGATGAAGAAGATCACTCTCCTCAACTATTTCCGCAATTACATGAGTGAGCACCTG CTGAAGGCAGGGGCCAACATCACACCCCGGGAAGGCGACGAGCTGGCCCGGCTGCCCTACCTACGAACGT GGTTCCGCACACGCAGCGCCATCATCCTGCACCTCAGCAACGGCACCGTGCAGATTAACTTCTTCCAGGA CCACACCAAACTTATCCTGTGCCCCCTGATGGCAGCGGTGACCTACATCAACGAGAAGAGGGACTTCCAA ACGTACCGCCTGAGCCTCCTGGAGGAGTATGGCTGCTGCAAGGAGCTGGCCAGCCGCCTCCGCTACGCCC GCACCATGGTAGACAAGCTGCTGAGCTCGCGCTCCGCCAGCAACCGCCTCAAGGCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209333 protein sequence
Red=Cloning site Green=Tags(s) MNAAAKAGKLARAPADLGKGGVPGDAVPGAPVAAPLAKEIPEVLVDPRSRRQYVRGRFLGKGGFAKCFEI SDADTKEVFAGKIVPKSLLLKPHQKEKMSMEISIHRSLAHQHVVGFHDFFEDSDFVFVVLELCRRRSLLE LHKRRKALTEPEARYYLRQIVLGCQYLHRNQVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYEGERKKT LCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAAS LIQKMLQTDPTARPTIHELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSSRKPLKVLNKGVENP LPDRPREKEEPVVRETNEAIECHLSDLLQQLTSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDK YGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLNYFRNYMSEHL LKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGTVQINFFQDHTKLILCPLMAAVTYINEKRDFQ TYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_011121 |
ORF Size | 1812 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011121.4 |
RefSeq Size | 2203 bp |
RefSeq ORF | 1812 bp |
Locus ID | 18817 |
UniProt ID | Q07832 |
MW | 68.3 kDa |
Gene Summary | The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and may play a role in DNA replication during S phase. This gene is expressed in all embryonic tissues, but restricted to thymus and ovaries in adult tissues. Homozygous knockout mice were embryonic lethal, suggesting that this gene is important for early embryonic development. This gene is thought to be a potential oncogene because it is overexpressed in a variety of tumors and tumor cell lines. Depletion of this protein in cancer cells has been shown to inhibit cell proliferation and suppress oncogenic transformation; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200395 | Plk1 (untagged) - Mouse polo-like kinase 1 (Drosophila) (Plk1), (10ug) |
CNY 4,496.00 |
|
MG209333 | Plk1 (tGFP-tagged) - Mouse polo-like kinase 1 (Drosophila) (Plk1) |
CNY 6,560.00 |
|
MR209333L3 | Lenti ORF clone of Plk1 (Myc-DDK-tagged) - Mouse polo-like kinase 1 (Drosophila) (Plk1) |
CNY 7,890.00 |
|
MR209333L4 | Lenti ORF clone of Plk1 (mGFP-tagged) - Mouse polo-like kinase 1 (Drosophila) (Plk1) |
CNY 7,890.00 |