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Snx1 (NM_019727) Mouse Tagged ORF Clone
CAT＃: MR208351
- TrueORF®
Snx1 (Myc-DDK-tagged) - Mouse sorting nexin 1 (Snx1)
ORF Plasmid: tGFP
"NM_019727" in other vectors (4)
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CNY 3,888.00
CNY 4,560.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208351 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCAGGAGGTGGTGGCTGTAGCGCTTCGGAGCGACTCCCTCCGCCCTTCCCTGGCATGGATCCGG AGTCGGAAGGGGCTGCCGGGGGGTCAGAACCCGAGGCTGGGGACAGCGACACCGAGGGGGAGGATATTTT CACTGGCGCCGCGGCAGCCACTAAGCCCCAGTCTCCAAAGAAAACCACATCCCTTTTTCCTATCAAAAAT GGTTCCAAAGAGAATGGGATCCATGAGGACCAAGACCAAGAGCCACAGGATCTCTTTGCAGATGCCACAG TGGAACTATCCCTGGACAGCACACAGAATAACCAGAAGACAATGCCAGGCAAAACCCTTACTTCTCATCC TCCGCAAGAAGCCACAAATTCTCCGAAGCCACAGCCAAGCTATGAGGAGCTGGAGGAAGAACAGGAGGAT CAGTTTGATTTGACAGTTGGTATCACCGATCCTGAGAAGATCGGGGATGGTATGAATGCCTATGTAGCCT ACAAAGTCACCACACAGACAAGCTTACCGATGTTCAGAAGTAGGCAGTTTGCAGTGAAAAGAAGATTCAG TGACTTCCTGGGTCTTTATGAGAAGCTTTCAGAGAAGCACTCCCAGAATGGTTTCATTGTGCCTCCTCCA CCCGAGAAGAGCCTAATAGGAATGACAAAGGTGAAGGTTGGGAAGGAAGATTCTTCTTCCGCAGAGTTTC TTGAGAAACGGAGGGCTGCTTTGGAAAGGTACCTTCAAAGAATTGTGAATCATCCCACCATGTTACAGGA CCCAGATGTCAGGGAGTTTCTGGAAAAAGAAGAGCTGCCTCGAGCTGTGGGCACGCAGGCTTTGAGTGGC GCTGGTCTCCTTAAGATGTTCAACAAAGCTACAGATGCAGTCAGCAAAATGACCATCAAGATGAATGAAT CAGACATTTGGTTCGAGGAGAAACTCCAGGAGGTAGAGTGTGAGGAGCAGCGCCTACGGAAGCTGCATGC TGTGGTGGAGACTCTAGTCAACCATAGGAAAGAACTAGCGCTGAACACAGCCCTGTTTGCCAAGAGTCTA GCCATGCTTGGGAGCTCCGAGGACAACACAGCACTTTCTCGAGCGCTCTCCCAACTGGCTGAGGTGGAAG AAAAGATTGAGCAGCTCCACCAGGAACAGGCCAACAATGACTTCTTCCTCCTTGCTGAACTCCTGAGTGA CTATATTCGCCTCCTGGCCATTGTCCGAGCTGCCTTCGACCAGCGCATGAAGACATGGCAGCGCTGGCAG GATGCTCAAGCCACGCTGCAGAAGAAGCGGGAGTCTGAGGCACGGCTGCTGTGGGCCAACAAGCCTGACA AACTGCAGCAGGCTAAGGATGAGATCACAGAGTGGGAATCTCGGGTGACTCAGTATGAAAGGGACTTTGA AAGGATTTCCACAGTTGTCCGAAAGGAAGTGACACGATTTGAGAAAGAGAAGTCCAAGGATTTCAAAAAC CACGTGATGAAGTACCTGGAGACACTCCTACATTCCCAGCAGCAGCTGGCAAAATACTGGGAAGCCTTCC TTCCTGAGGCAAAGGCCATCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208351 protein sequence
Red=Cloning site Green=Tags(s) MASGGGGCSASERLPPPFPGMDPESEGAAGGSEPEAGDSDTEGEDIFTGAAAATKPQSPKKTTSLFPIKN GSKENGIHEDQDQEPQDLFADATVELSLDSTQNNQKTMPGKTLTSHPPQEATNSPKPQPSYEELEEEQED QFDLTVGITDPEKIGDGMNAYVAYKVTTQTSLPMFRSRQFAVKRRFSDFLGLYEKLSEKHSQNGFIVPPP PEKSLIGMTKVKVGKEDSSSAEFLEKRRAALERYLQRIVNHPTMLQDPDVREFLEKEELPRAVGTQALSG AGLLKMFNKATDAVSKMTIKMNESDIWFEEKLQEVECEEQRLRKLHAVVETLVNHRKELALNTALFAKSL AMLGSSEDNTALSRALSQLAEVEEKIEQLHQEQANNDFFLLAELLSDYIRLLAIVRAAFDQRMKTWQRWQ DAQATLQKKRESEARLLWANKPDKLQQAKDEITEWESRVTQYERDFERISTVVRKEVTRFEKEKSKDFKN HVMKYLETLLHSQQQLAKYWEAFLPEAKAIS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019727 |
| ORF Size | 1566 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019727.2, NP_062701.2 |
| RefSeq Size | 2079 bp |
| RefSeq ORF | 1566 bp |
| Locus ID | 56440 |
| UniProt ID | Q9WV80 |
| MW | 58.9 kDa |
| Gene Summary | Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC). Can sense membrane curvature and has in vitro vesicle-to-membrane remodeling activity. Involved in retrograde endosome-to-TGN transport of lysosomal enzyme receptors (IGF2R, M6PR and SORT1). Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Involvement in retromer-independent endocytic trafficking of P2RY1 and lysosomal degradation of protease-activated receptor-1/F2R. Promotes KALRN- and RHOG-dependent but retromer-independent membrane remodeling such as lamellipodium formation; the function is dependent on GEF activity of KALRN. Required for endocytosis of DRD5 upon agonist stimulation but not for basal receptor trafficking (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A critical role for Sorting Nexin 1 in the trafficking of metabotropic glutamate receptors
,Sharma, R;Gulia, R;Bhattacharyya, S;,
J. Neurosci.
,PubMed ID 30143569
[SNX1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202614 | Snx1 (untagged) - Mouse sorting nexin 1 (Snx1), (10ug) |
CNY 3,888.00 |
|
| MG208351 | Snx1 (tGFP-tagged) - Mouse sorting nexin 1 (Snx1) |
CNY 5,040.00 |
|
| MR208351L3 | Lenti ORF clone of Snx1 (Myc-DDK-tagged) - Mouse sorting nexin 1 (Snx1) |
CNY 6,460.00 |
|
| MR208351L4 | Lenti ORF clone of Snx1 (mGFP-tagged) - Mouse sorting nexin 1 (Snx1) |
CNY 6,288.00 |
