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Epsti1 (BC020120) Mouse Tagged ORF Clone
CAT＃: MR204487
- TrueORF®
Epsti1 (Myc-DDK-tagged) - Mouse epithelial stromal interaction 1 (breast) (cDNA clone MGC:28189 IMAGE:3988503)
ORF Plasmid: tGFP
"BC020120" in other vectors (3)
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CNY 2,400.00
CNY 6,365.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2310046K10Rik; 5033415K03Rik; BRESI1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204487 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACACCCGCAGTAAGGTGGTGGGCCCAGGCTTGGGCACTTCCTCCATCTCTCGGGACCACGCTGGAG CTGGGCAGCGCAGGGAGCTGGGCTTGCAGCAAAACCGGAGACAGAGTTTGGAGGTGGCAGCTCCCGAGGG CCCCAAGATGGAGAGGCAGGGGCATGCTGACCAGGGAAGCGCAGGCACATACACCCTGATAGCACCGAAC GAGAGCAGAAGGCAGAAGATACAGAGAATTGCTGAGCAAGAACTGGCAGACCTGGAGAGATGGAAGCAAC AGAACAGGGCAAAACCAGTCTACTTGGTGCCACAACGGCTGGGTGGAAGCCAGTCTGAGGCTGAAGTCAG ACAGAAACAACAGCTGCAACAGATGCGTTCTAAATACCAGCAAAAGCTCAAGAGGGATGAATCTATTCGA ATCAGGAAGGAAGCTGAGGAAGCCAAGTTCCAAAAAATGAAAGCAATCCAGAGGGAGAAGAGTAATAAAC TAGAGGAGAAAAAGCAACTTCAAGAAGACATCAGAAGAGCCAAGTTTCGAGAGCATCATCAGTCCAAAAC GGCTGAGTTCTTGAGCAGACTGGACACAGAGCGGAGAAACAGAAGTGCCTGTCCGATTGCTCCTCCTGCC ACCCAGTCCTCGAGATGGAAACTTCCAGTCCTGCTTAAAGATCCCAGCTGGGCCGGCAGCCAAGCACACA GGGATTCTCCGCAGAAGGAAGACAACCCCAGGCTGCAAAAGACGAGGGATGGACATCAGAAGAATAAATT ACTGGAAACTAAAGGACAGCACCAGGAGGAAGAAAGAGCCCAAATCCATCAAGCTGAACACTGGAGGGTA AATAATGCTTTTCTGGACCGACTCCAAGGCAAAAGTCAACCAGGTGGCCTCAAGCAGTCTGGAGGCTGTT GCAATATGAATAGTACTGACAGCTGGGGTATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204487 protein sequence
Red=Cloning site Green=Tags(s) MYTRSKVVGPGLGTSSISRDHAGAGQRRELGLQQNRRQSLEVAAPEGPKMERQGHADQGSAGTYTLIAPN ESRRQKIQRIAEQELADLERWKQQNRAKPVYLVPQRLGGSQSEAEVRQKQQLQQMRSKYQQKLKRDESIR IRKEAEEAKFQKMKAIQREKSNKLEEKKQLQEDIRRAKFREHHQSKTAEFLSRLDTERRNRSACPIAPPA TQSSRWKLPVLLKDPSWAGSQAHRDSPQKEDNPRLQKTRDGHQKNKLLETKGQHQEEERAQIHQAEHWRV NNAFLDRLQGKSQPGGLKQSGGCCNMNSTDSWGI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC020120 |
| ORF Size | 942 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC020120, AAH20120 |
| RefSeq Size | 1719 bp |
| RefSeq ORF | 944 bp |
| Locus ID | 108670 |
| MW | 36.1 kDa |
| Gene Summary | Plays a role in M1 macrophage polarization and is required for the proper regulation of gene expression during M1 versus M2 macrophage differentiation (PubMed:29217193). Might play a role in RELA/p65 and STAT1 phosphorylation and nuclear localization upon activation of macrophages (PubMed:29217193).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG204487 | Epsti1 (tGFP-tagged) - Mouse epithelial stromal interaction 1 (breast) (cDNA clone MGC:28189 IMAGE:3988503) |
CNY 2,850.00 |
|
| MR204487L3 | Lenti ORF clone of Epsti1 (Myc-DDK-tagged) - Mouse epithelial stromal interaction 1 (breast) (cDNA clone MGC:28189 IMAGE:3988503) |
CNY 4,750.00 |
|
| MR204487L4 | Lenti ORF clone of Epsti1 (mGFP-tagged) - Mouse epithelial stromal interaction 1 (breast) (cDNA clone MGC:28189 IMAGE:3988503) |
CNY 4,750.00 |
