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Ehf (BC005520) Mouse Tagged ORF Clone
CAT＃: MR203873
- TrueORF®
Ehf (Myc-DDK-tagged) - Mouse ets homologous factor (cDNA clone MGC:7616 IMAGE:3494644)
ORF Plasmid: tGFP
"BC005520" in other vectors (4)
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CNY 2,400.00
CNY 6,365.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9030625L19Rik; AU019492 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203873 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCTGGAAGGAAGTGGTGTAATGAATCTCAACCCAGCCAACAACCTCCTTCACCAGCAACCAGCCT GGACGGACAGCTACCCCACATGCAATGTTTCCAGCGGTTTTTTTGGAAGCCAGTGGCATGAAATCCACCC TCAGTACTGGACCAAATACCAGGTGTGGGAATGGCTGCAGCACCTCCTGGACACCAACCAGCTGGACGCT AGCTGCATCCCTTTCCAGGAGTTCACGAGGGCAGCAGGCTCAGCTGGGCAGCTGCTCTACAGCAACCTAC AGCATCTCAAGTGGAATGGCCAATGCAGCAGTGACCTTTTCCAGTCCGCACACAATGTCATTGTCAAGAC TGAACAAACCGATCCTTCCATCATGAACACATGGAAAGAAGAAAACTATCTCTATGATCCCAGCTATGGT AGCACAGTAGATCTGTTGGACAGTAAGACTTTCTGCCGGGCTCAGATCTCCATGACAACCTCCAGTCACC TTCCAGTTGCAGAGTCACCTGATATGAAAAAGGAGCAAGACCACCCTGTAAAGTCCCACACCAAAAAGCA CAACCCAAGAGGCACTCACTTATGGGAGTTCATCCGAGACATTCTCCTGAGCCCAGACAAGAACCCAGGA CTGATCAAATGGGAAGACCGTTCGGAAGGCATCTTCAGGTTCCTGAAGTCAGAAGCTGTGGCTCAGCTGT GGGGGAAAAAGAAAAATAACAGTAGCATGACATACGAGAAGCTCAGCCGGGCTATGAGATATTACTACAA ACGAGAAATCCTGGAACGTGTGGATGGACGACGGCTGGTCTATAAGTTTGGGAAGAATGCTCGTGGATGG AGAGAAAATGAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203873 protein sequence
Red=Cloning site Green=Tags(s) MILEGSGVMNLNPANNLLHQQPAWTDSYPTCNVSSGFFGSQWHEIHPQYWTKYQVWEWLQHLLDTNQLDA SCIPFQEFTRAAGSAGQLLYSNLQHLKWNGQCSSDLFQSAHNVIVKTEQTDPSIMNTWKEENYLYDPSYG STVDLLDSKTFCRAQISMTTSSHLPVAESPDMKKEQDHPVKSHTKKHNPRGTHLWEFIRDILLSPDKNPG LIKWEDRSEGIFRFLKSEAVAQLWGKKKNNSSMTYEKLSRAMRYYYKREILERVDGRRLVYKFGKNARGW RENEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC005520 |
| ORF Size | 855 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC005520, AAH05520 |
| RefSeq Size | 3746 bp |
| RefSeq ORF | 857 bp |
| Locus ID | 13661 |
| MW | 33.2 kDa |
| Gene Summary | Transcriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. May act as a repressor for a specific subset of ETS/AP-1-responsive genes, and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. Binds to DNA sequences containing the consensus nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218089 | Ehf (untagged) - Mouse ets homologous factor (cDNA clone MGC:7616 IMAGE:3494644), (10ug) |
CNY 6,940.00 |
|
| MG203873 | Ehf (tGFP-tagged) - Mouse ets homologous factor (cDNA clone MGC:7616 IMAGE:3494644) |
CNY 2,850.00 |
|
| MR203873L3 | Lenti ORF clone of Ehf (Myc-DDK-tagged) - Mouse ets homologous factor (cDNA clone MGC:7616 IMAGE:3494644) |
CNY 4,750.00 |
|
| MR203873L4 | Lenti ORF clone of Ehf (mGFP-tagged) - Mouse ets homologous factor (cDNA clone MGC:7616 IMAGE:3494644) |
CNY 4,750.00 |
