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Hlf (BC057693) Mouse Tagged ORF Clone
CAT＃: MR202293
- TrueORF®
Hlf (Myc-DDK-tagged) - Mouse hepatic leukemia factor (cDNA clone MGC:68394 IMAGE:4196259)
ORF Plasmid: tGFP
"BC057693" in other vectors (3)
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CNY 2,400.00
CNY 2,945.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | E230015K02Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR202293 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTTGGAGGAATTCCTGTCAGAGAATGGCATCCCCCCGAGTCCGTCGCAGCACGACCACAGCCCTC ACCCCCCTGGCTTGCAACCAGCTTCCTCCACGGCGCCCTCGGTCATGGATCTCAGCAGCCGGGCCACAGC GCCCCTCCACCCGGGCATCCCGTCTCCGAACTGTATGCAGAGCCCCATCAGACCAGGCCAGCTGTTGCCA GCAAACCGCAATACACCGAGTCCCATTGACCCTGACACCATCCAGGTACCAGTCGGGTATGAACCAGACC CGGCAGACCTTGCCCTCTCCAGCATCCCGGGGCAGGAAATGTTTGACCCTCGCAAACGGAAGTTCTCTGA GGAAGAACTGAAGCCACAGCCCATGATTAAGAAAGCTCGCAAAGTCTTCATTCCCGATGATTTGAAGCAG GATGACAAGTACTGGGCGAGGCGCAGAAAGAACAACATGGCGGCCAAGCGCTCCCGTGATGCCCGGCGGC TGAAGGAGAACCAGATCGCAATCCGGGCCTCATTCCTGGAGAAGGAGAACTCGGCCCTCCGCCAGGAGGT GGCTGATTTAAGGAAGGAGCTGGGCAAATGCAAGAACATACTTGCCAAGTACGAGGCCAGGCACGGGCCC CTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202293 protein sequence
Red=Cloning site Green=Tags(s) MDLEEFLSENGIPPSPSQHDHSPHPPGLQPASSTAPSVMDLSSRATAPLHPGIPSPNCMQSPIRPGQLLP ANRNTPSPIDPDTIQVPVGYEPDPADLALSSIPGQEMFDPRKRKFSEEELKPQPMIKKARKVFIPDDLKQ DDKYWARRRKNNMAAKRSRDARRLKENQIAIRASFLEKENSALRQEVADLRKELGKCKNILAKYEARHGP L TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC057693 |
| ORF Size | 633 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC057693, AAH57693 |
| RefSeq Size | 2480 bp |
| RefSeq ORF | 635 bp |
| Locus ID | 217082 |
| MW | 23.6 kDa |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Co-occupancy identifies transcription factor co-operation for axon growth
,Venkatesh, I;Mehra, V;Wang, Z;Simpson, MT;Eastwood, E;Chakraborty, A;Beine, Z;Gross, D;Cabahug, M;Olson, G;Blackmore, MG;,
Nature communications
,PubMed ID 33953205
[HLF]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG202293 | Hlf (tGFP-tagged) - Mouse hepatic leukemia factor (cDNA clone MGC:68394 IMAGE:4196259) |
CNY 2,850.00 |
|
| MR202293L3 | Lenti ORF clone of Hlf (Myc-DDK-tagged) - Mouse hepatic leukemia factor (cDNA clone MGC:68394 IMAGE:4196259) |
CNY 4,750.00 |
|
| MR202293L4 | Lenti ORF clone of Hlf (mGFP-tagged) - Mouse hepatic leukemia factor (cDNA clone MGC:68394 IMAGE:4196259) |
CNY 4,750.00 |
