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1110005A23Rik (BC027510) Mouse Tagged ORF Clone
CAT＃: MR202272
- TrueORF®
1110005A23Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110005A23 gene (cDNA clone MGC:41057 IMAGE:1382354)
ORF Plasmid: tGFP
"BC027510" in other vectors (4)
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CNY 2,400.00
CNY 2,945.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110005A23Rik; Cip29 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR202272 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCGAGACGGTGGAGCTCCACAAGCTGAAGCTTGCTGAACTAAAGCAAGAATGTCTTGCTCGTG GTTTAGAGACCAAGGGAATAAAACAAGATCTTATAAATAGGCTGCAGGCCTATCTTGAAGACCATGCAGA AGAAGAAGCAAATGAAGAAGATGTACTGGGAGATGAAACTGAGGAAGAAGAACCAAAGCCCATAGAACTG CCTGTTAAAGAGGAAGAACCTCCTGAAAAGGCTGTTGATATGGCATCGGAAAAGAAGGTGGTAAAGATTA CATCTGGGATACCTCAGACTGAAAGAATGCAGAAGAGGGCTGAACGATTCAATGTACCTGTAAGCCTGGA GAGTAAGAAGGCTGCCCGGGCGGCTAGGTTTGGAATTTCTTCAGTTCCAACAAAAGGTTTATCATCTGAC ACCAAGCCTATGGTTAACCTGGATAAACTAAAGGAAAGAGCACAGAGATTTGGTTTGAATGTCTCTTCCA TCTCTAGAAAGTCTGAGGATGATGAGAAGCTGAAGAAACGGAAGGAGAGATTTGGGATTGTGACAAGTTC AGCTGGAACTGGAACCACAGAGGATACAGAGGCAAAGAAAAGAAAAAGAGCAGAGCGTTTTGGAATTGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202272 protein sequence
Red=Cloning site Green=Tags(s) MAAETVELHKLKLAELKQECLARGLETKGIKQDLINRLQAYLEDHAEEEANEEDVLGDETEEEEPKPIEL PVKEEEPPEKAVDMASEKKVVKITSGIPQTERMQKRAERFNVPVSLESKKAARAARFGISSVPTKGLSSD TKPMVNLDKLKERAQRFGLNVSSISRKSEDDEKLKKRKERFGIVTSSAGTGTTEDTEAKKRKRAERFGIA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC027510 |
| ORF Size | 630 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC027510, AAH27510 |
| RefSeq Size | 908 bp |
| RefSeq ORF | 632 bp |
| Locus ID | 66118 |
| MW | 23.5 kDa |
| Gene Summary | Binds both single-stranded and double-stranded DNA with higher affinity for the single-stranded form. Specifically binds to scaffold/matrix attachment region DNA. Also binds single-stranded RNA. Enhances RNA unwinding activity of DDX39A. May participate in important transcriptional or translational control of cell growth, metabolism and carcinogenesis. Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC206894 | 1110005A23Rik (untagged) - Mouse RIKEN cDNA 1110005A23 gene (cDNA clone MGC:41057 IMAGE:1382354), (10ug) |
CNY 3,230.00 |
|
| MG202272 | 1110005A23Rik (tGFP-tagged) - Mouse RIKEN cDNA 1110005A23 gene (cDNA clone MGC:41057 IMAGE:1382354) |
CNY 2,850.00 |
|
| MR202272L3 | Lenti ORF clone of 1110005A23Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110005A23 gene (cDNA clone MGC:41057 IMAGE:1382354) |
CNY 4,750.00 |
|
| MR202272L4 | Lenti ORF clone of 1110005A23Rik (mGFP-tagged) - Mouse RIKEN cDNA 1110005A23 gene (cDNA clone MGC:41057 IMAGE:1382354) |
CNY 4,750.00 |
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