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Upf2 (NM_001081132) Mouse Tagged ORF Clone
CAT＃: MG226926
- TrueORF®
Upf2 (tGFP-tagged) - Mouse UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), (10ug). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_001081132" in other vectors (4)
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CNY 13,870.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226926 representing NM_001081132, codon optimized.
Due to the complexity of NM_001081132, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGCCGAGCGCAAGAAATCCGCCTCAATGGAAGAAAAGGAATCCCTGCTGAACAATAAGGAGAAAG ACTGCTCCGAGAGACGCCCTGTGAGCTCAAAGGAAAAGCCAAGAGATGATCTGAAAGTTACAGCCAAAAA GGAGGTCAGCAAAGTACCCGAGGATAAAAAAAAGAGACTCGAAGAGGATAAAAGGAAAAAAGAGGATAAA GAGCGGAAAAAGAAAGAGGAAGAGAAGGTGAAGGCCGAAGAGGAATTGAAAAAGAAGGAGGAGGAAGAGA AGAAAAAGCAAGAAGAGGAGGAGCGCAAAAAACAGGAGGAACAGGCTAAACGGCAACAGGAGGAGGCCGC TGCCCAGCTGAAGGAGAAGGAGGAATCCCTTCAGCTGCACCAAGAGGCCTGGGAAAGACACCAACTGAGA AAAGAGCTGAGATCCAAAAACCAGAACGCCCCTGATAATAGACCCGAGGAGAACTTCTTCAGTAGACTGG ATTCTTCCCTGAAGAAGAATACCGCCTTCGTTAAGAAATTGAAAACAATCACCGAGCAACAGCGCGACAG CCTGAGTCACGATTTCAACGGTCTTAACCTGTCAAAGTATATTGCCGAAGCAGTAGCATCAATTGTTGAG GCAAAACTGAAACTGAGCGATGTGAACTGTGCGGCTCACTTGTGCTCCCTCTTCCACCAGAGATATTCCG ATTTTGCTCCGAGTTTGCTGCAGGTGTGGAAAAAGCACTTTGAGGCGAGGAAGGAAGAGAAGACACCCAA CATCACTAAGCTCAGGACAGACCTGCGGTTCATTGCCGAACTGACCATAGTTGGTATCTTCACAGACAAA GAGGGCCTCAGTCTCATCTACGAGCAGCTGAAGAGCATCATTAATGCCGATCGGGAGTCACACACGCACG TCTCAGTCGTGATCAGTTTCTGTCGCCACTGCGGCGACGACATCGCAGGACTGGTTCCAAGGAAGGTTAA GTCCGCGGCGGAGAAGTTTAACCTGTCCTTCCCACCATCTGAAATCATTTCACCTGAAAAGCAGCAGCCA TTCCAGAATCTCCTCAAGGAGTACTTTACGAGCTTGACAAAGCACCTGAAGAGGGACCACCGGGAGCTTC AAAACACAGAACGCCAGAACCGGCGCATACTGCATTCTAAAGGAGAACTGAGTGAGGATCGACACAAGCA GTATGAAGAATTCGCTATGTCCTACCAGAAGCTCTTGGCTAACAGCCAGTCACTTGCGGACTTGCTGGAC GAGAACATGCCAGATCTGCCCCAAGACAAACCCACCCCTGAGGAGCACGGTCCAGGTATCGATATTTTCA CACCAGGGAAACCTGGCGAGTATGATCTCGAGGGCGGGATCTGGGAAGATGAAGATGCCAGAAATTTCTA CGAGAACCTGATTGACCTGAAGGCCTTCGTGCCAGCCATCCTGTTCAAAGATAACGAGAAATCTCAGAAC AAGGACAGTAACAAGGATGACAGTAAAGAGGCCAAAGAACCCAAGGACAATAAAGAGGCTAGCTCCCCAG ACGACCTTGAACTCGAACTTGAGAACCTGGAGATCAACGATGACACGCTTGAGCTGGAAGGAGCAGACGA GGCGGAAGATCTGACCAAGAAGCTGCTTGATGAGCAGGAACAAGAGGACGAAGAAGCCTCTACAGGGAGC CACCTGAAATTGATCGTAGACGCCTTCCTGCAGCAGCTCCCCAACTGCGTGAACCGAGATCTCATAGACA AGGCAGCCATGGATTTCTGTATGAACATGAACACAAAGGCAAATCGCAAGAAGCTGGTGCGGGCTCTCTT CATCGTACCCCGCCAGAGGCTGGATTTGCTTCCCTTCTATGCTAGGTTGGTGGCGACCCTTCACCCTTGT ATGAGTGACGTGGCAGAAGATTTGTGTAGCATGCTGCGAGGGGATTTTCGATTCCATGTGAGAAAGAAAG ATCAGATAAACATTGAGACTAAGAATAAGACCGTTAGATTTATTGGGGAGCTCACCAAATTTAAAATGTT CACCAAAAACGATACACTCCATTGCCTGAAAATGCTCTTGAGTGACTTCTCTCATCACCATATCGAAATG GCGTGCACACTGCTGGAGACGTGCGGTCGGTTTCTGTTTCGGAGCCCGGAATCTCATCTGAGGACCTCAG TGTTGCTGGAACAGATGATGAGGAAAAAGCAGGCAATGCACTTGGATGCTCGGTATGTGACCATGGTGGA AAACGCCTACTATTATTGCAACCCACCGCCTGCCGAAAAGACAGTGCGGAAGAAAAGGCCCCCCCTGCAG GAGTATGTCCGGAAACTTTTGTACAAGGATCTGAGTAAGGTAACCACTGAGAAGGTCTTGCGGCAGATGC GCAAGCTGCCTTGGCAAGATCAGGAGGTTAAGGATTATGTCATATGTTGTATGATAAATATCTGGAATGT GAAGTACAATTCCATTCATTGCGTGGCAAACCTGCTCGCGGGCCTCGTACTTTACCAGGAGGATGTCGGC ATACATGTTGTGGACGGAGTACTCGAAGACATCCGGTTGGGCATGGAAGTGAATCAGCCGAAGTTCAACC AGCGCAGGATATCTTCCGCTAAGTTTTTGGGCGAACTGTATAATTACCGCATGGTTGAAAGTGCAGTGAT CTTCAGAACGTTGTACTCCTTCACTTCCTTCGGAGTGAACCCCGACGGCAGCCCTTCTTCTCTCGACCCT CCTGAGCACCTGTTTCGGATCAGGCTGGTGTGTACCATCCTCGACACGTGTGGCCAATACTTTGATCGAG GCTCTAGTAAACGCAAGCTCGACTGTTTTCTCGTATACTTCCAGCGGTACGTCTGGTGGAAGAAAAGTCT GGAGGTGTGGACAAAGGACCACCCTTTCCCTATTGACATTGACTACATGATCTCCGACACTCTGGAACTG TTGCGACCCAAAATAAAGCTGTGTAATAGCTTGGAGGAGAGCATCCGCCAGGTACAGGATCTTGAACGGG AATTCCTCATAAAGCTGGGCCTGGTAAACGACAAAGAGTCTAAGGACTCCATGACAGAAGGGGAAAATCT GGAGGAGGACGAGGAAGAAGAGGAGGGAGGCGCCGAGACAGAAGAGCAATCTGGCAACGAGAGTGAGGTG AACGAGCCTGAGGAGGAGGAAGGCTCTGAAGAGGAAGAAGAGGGAGAAGAAGAGGAGGAGGAGAACACTG ATTACCTGACAGATTCTAATAAGGAGAATGAAACCGACGAGGAAAACGCGGAGGTGATGATTAAAGGTGG AGGATTGAAGCACGTTCCTTGCGTTGAGGACGAGGACTTCATTCAGGCCCTGGATAAAATGATGTTGGAG AACCTTCAGCAGAGAAGCGGAGAGTCCGTTAAAGTGCATCAGCTGGACGTGGCCATCCCCCTGCATCTGA AGTCCCAACTCCGCAAGGGGCCGCCTCTGGGTGGCGGCGAAGGAGAGACAGAGTCTGCTGACACAATGCC ATTTGTGATGTTGACAAGAAAGGGTAATAAGCAGCAGTTCAAGATCTTGAATGTCCCCATGTCTTCCCAG TTGGCCGCCAATCACTGGAATCAGCAACAGGCCGAACAGGAGGAGAGAATGAGGATGAAAAAGCTGACAC TTGACATTAACGAGAGACAGGAACAGGAGGACTATCAAGAGATGTTGCAGTCACTGGCCCAGCGCCCAGC CCCTGCAAATACAAATAGAGAGCGCAGGCCTAGGTATCAACACCCTAAGGGGGCACCAAACGCTGATCTG ATTTTCAAGACAGGTGGCAGAAGAAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226926 representing NM_001081132
Red=Cloning site Green=Tags(s) MPAERKKSASMEEKESLLNNKEKDCSERRPVSSKEKPRDDLKVTAKKEVSKVPEDKKKRLEEDKRKKEDK ERKKKEEEKVKAEEELKKKEEEEKKKQEEEERKKQEEQAKRQQEEAAAQLKEKEESLQLHQEAWERHQLR KELRSKNQNAPDNRPEENFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNGLNLSKYIAEAVASIVE AKLKLSDVNCAAHLCSLFHQRYSDFAPSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIVGIFTDK EGLSLIYEQLKSIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKSAAEKFNLSFPPSEIISPEKQQP FQNLLKEYFTSLTKHLKRDHRELQNTERQNRRILHSKGELSEDRHKQYEEFAMSYQKLLANSQSLADLLD ENMPDLPQDKPTPEEHGPGIDIFTPGKPGEYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKSQN KDSNKDDSKEAKEPKDNKEASSPDDLELELENLEINDDTLELEGADEAEDLTKKLLDEQEQEDEEASTGS HLKLIVDAFLQQLPNCVNRDLIDKAAMDFCMNMNTKANRKKLVRALFIVPRQRLDLLPFYARLVATLHPC MSDVAEDLCSMLRGDFRFHVRKKDQINIETKNKTVRFIGELTKFKMFTKNDTLHCLKMLLSDFSHHHIEM ACTLLETCGRFLFRSPESHLRTSVLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVRKKRPPLQ EYVRKLLYKDLSKVTTEKVLRQMRKLPWQDQEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQEDVG IHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFTSFGVNPDGSPSSLDP PEHLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLEVWTKDHPFPIDIDYMISDTLEL LRPKIKLCNSLEESIRQVQDLEREFLIKLGLVNDKESKDSMTEGENLEEDEEEEEGGAETEEQSGNESEV NEPEEEEGSEEEEEGEEEEEENTDYLTDSNKENETDEENAEVMIKGGGLKHVPCVEDEDFIQALDKMMLE NLQQRSGESVKVHQLDVAIPLHLKSQLRKGPPLGGGEGETESADTMPFVMLTRKGNKQQFKILNVPMSSQ LAANHWNQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADL IFKTGGRRR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081132 |
| ORF Size | 3807 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081132.1, NP_001074601.1 |
| RefSeq Size | 5174 bp |
| RefSeq ORF | 3810 bp |
| Locus ID | 326622 |
| UniProt ID | A2AT37 |
| Gene Summary | Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). Recruited by UPF3B associated with the EJC core at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF3B stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224119 | Upf2 (untagged) - Mouse UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), (10ug) |
CNY 16,340.00 |
|
| MR226926 | Upf2 (Myc-DDK-tagged) - Mouse UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2). Note: ORF is codon optimized |
CNY 8,840.00 |
|
| MR226926L3 | Lenti ORF clone of Upf2 (Myc-DDK-tagged) - Mouse UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2). Note: ORF is codon optimized |
CNY 14,540.00 |
|
| MR226926L4 | Lenti ORF clone of Upf2 (mGFP-tagged) - Mouse UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2). Note: ORF is codon optimized |
CNY 14,540.00 |
