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Myh8 (NM_177369) Mouse Tagged ORF Clone
CAT＃: MG220412
- TrueORF®
Myh8 (tGFP-tagged) - Mouse myosin heavy polypeptide 8 skeletal muscle perinatal (Myh8), (10ug)
ORF Plasmid: DDK
"NM_177369" in other vectors (2)
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CNY 23,370.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4832426G23Rik; AI327267; MHCp; Myh; MyHC-; MyHC-pn; Myhs; Myhs-p; Myhsp |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220412 representing NM_177369
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTACGGGTTCGGACGCAGAGATGGCTATTTTTGGCGAAGCTGCTCCTTACCTCCGAAAATCGGAGA AGGAGAGGATTGAGGCCCAAAATAAGCCTTTTGACGCAAAAACATCTGTCTTTGTGGCGGAGCCCAAGGA GTCTTATGTGAAAAGCGTTATACAAAGCAAGGATGGAGGGAAAGTGACCGTGAAGACGGAAAGTGGAGCA ACTCTCACCGTCAAGGAAGATCAAGTCTTCCCCATGAACCCTCCCAAATATGACAAGATCGAGGACATGG CCATGATGACCCACCTCCATGAGCCCGGAGTGCTGTACAACCTCAAAGAGCGTTATGCAGCCTGGATGAT CTATACCTACTCAGGCCTCTTCTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAACCCTGAG GTGGTGGCAGCCTACCGAGGCAAAAAGCGCCAGGAGGCCCCACCCCACATCTTCTCCATCTCTGACAACG CCTACCAGTTCATGCTGACGGATCGTGAGAACCAGTCCATCCTGATCACCGGAGAATCCGGGGCCGGGAA GACTGTGAACACGAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTCACTGGGGACAAGAAGAAGGAG GAGTCTGGCAAAATGCAGGGGACCCTGGAGGATCAAATCATCAGCGCTAACCCCCTGCTGGAGGCCTTTG GGAACGCCAAGACCGTGAGGAACGACAACTCGTCTCGCTTTGGCAAGTTCATCAGAATCCACTTTGGTAC CACGGGGAAACTGGCATCAGCTGATATTGAAACCTATCTTCTAGAGAAGTCTAGAGTCACTTTCCAGCTA AAAGCTGAAAGAAGTTACCACATTTTTTATCAGATCACCTCCAATAAGAAGCCAGAGCTAATTGAAATGC TCCTGATCACCACCAACCCATACGACTACGCCTTTGTCAGTCAGGGGGAGATCACGGTGCCCAGCATTGA TGACCAAGAAGAGTTGATGGCCACAGATAGTGCCATTGATATCCTTGGCTTCTCACCTGAAGAGAAAGTG TCCATCTATAAACTCACAGGGGCTGTGATGCATTATGGGAACATGAAGTTCAAGCAAAAGCAGCGGGAGG AGCAGGCTGAGCCAGATGGCACTGAAGTGGCTGACAAGGCTGCCTATCTCCAGTGTCTGAACTCTGCTGA CCTGCTCAAAGCCCTCTGCTATCCCAGGGTCAAGGTTGGCAATGAATATGTCACCAAAGGCCAGACGGTT CAACAAGTGTATAATGCGGTGGGTGCCCTGGCCAAGGCTGTCTATGAGAAGATGTTCCTGTGGATGGTCA CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCCGGCAGTACTTCATCGGGGTCTTGGACATCGCTGG CTTTGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAATGAGAAACTGCAACAG TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGACCTTCA TCGACTTCGGGATGGACCTGGCGGCCTGCATCGAGCTCATTGAGAAGCCACTGGGCATCTTCTCCATCCT GGAAGAAGAGTGCATGTTCCCTAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTACGACCAACATCTG GGAAAATCCAACAACTTCCAGAAGCCCAAGCCTGCTAAAGGCAAGGCCGAGGCCCACTTCTCCCTGGTGC ACTATGCGGGCACTGTGGATTACAACATCACCGGCTGGCTGGACAAGAACAAGGACCCCCTGAATGACAC CGTGGTGGGGCTGTACCAGAAGTCAGCAATGAAAACTCTGGCCAGCCTCTTTTCCACATATGCTAGTGCT GAAGCAGATGGTGGTGCAAAGAAAGGAGCTAAGAAGAAGGGCTCTTCTTTCCAAACTGTGTCAGCCCTTT TCAGGGAAAATTTAAATAAACTGATGACCAATCTGAGAAGTACACACCCTCACTTCGTACGGTGTATCAT TCCCAATGAAACGAAGACTCCCGGTGCCATGGAGCACGAACTGGTCCTGCACCAGCTGAGGTGTAACGGG GTGCTGGAGGGCATCCGCATCTGCAGGAAGGGGTTCCCCAGCCGGATCCTGTATGGGGACTTTAAGCAGA GATACAAGGTTTTAAATGCAAGTGCTATTCCAGAGGGACAGTTCATCGACAGCAAGAAGGCCTCTGAGAA GCTTCTGGGCTCTATTGATATCGATCACACTCAGTATAAATTTGGACACACCAAGGTTTTCTTCAAAGCT GGTCTCCTGGGTCTCCTGGAAGAAATGAGAGATGAAAAGCTGGCCCAGATTATAACAAGAACCCAGGCGG TCTGTAGGGGATACCTAATGAGAGTTGAGTATCAGAAGATGCTGCTGAGAAGAGAATCCATCTTCTGCAT CCAGTACAACGTCCGCGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTCTTCAAGATCAAG CCCCTGCTGAAGAGCGCAGAGACTGAGAAGGAGATGGCCACCATGAAGGAGGAGTTCCAGAAAACCAAGG ATGAGCTGGCCAAGTCGGAGGCAAAGCGGAAGGAGCTGGAGGAGAAGATGGTCACTCTTCTCAAAGAGAA AAATGACCTGCAACTCCAGGTTCAGTCTGAAGCAGACAGCTTGGCCGATGCTGAGGAAAGGTGTGAACAA TTGATCAAAAACAAAATCCAGCTGGAGGCCAAAATCAAAGAGGTGACCGAGAGAGCCGAGGACGAGGAGG AGATCAACGCAGAGCTCACGGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCAGAGCTAAAGAAGGACAT CGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACAGAGAACAAGGTGAAAAAC CTGACAGAGGAGATGGCAGGCCTGGACGAAACTATCGCCAAGCTGACCAAGGAGAAGAAGGCCCTCCAGG AGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCTGACCAAAGCCAA AACTAAGCTGGAACAGCAAGTGGATGATCTTGAGGGATCGCTAGAGCAAGAAAAGAAGCTGAGGATGGAC TTAGAAAGAGCCAAGAGGAAACTGGAGGGGGACCTGAAACTGGCCCAAGAGTCCACAATGGACATAGAAA ACGATAAACAGCAGCTCGATGAGAAACTCAAAAAGAAGGAGTTTGAAATCAGCAACCTGATAAGCAAAAT TGAAGATGAGCAAGCCGTGGAGATCCAGCTGCAGAAGAAGATCAAAGAGTTGCAGGCCCGCATCGAGGAG CTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCAGGGCCAAAGCAGAGAAGCAGCGCTCTGACCTCTCCC GGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCCGGCGGGGCCACCTCTGCTCAGGTGGAGATGAA CAAGAAGCGGGAAACTGAGTTTCAGAAACTTCGCCGGGACCTGGAGGAGGCCACGCTGCAGCACGAAGCC ACAGCAGCCGCTCTTCGGAAGAAGCACGCGGACAGCGTGGCTGAGCTTGGGGAGCAGATCGACAACCTGC AGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGCTGAAGATGGAGATCGATGACCTTAGCAGTAA TGCAGAGGCCATCGCCAAAGCCAAGGGAAACCTGGAGAAGATGTGCCGCACTCTGGAGGACCAGGTGAGT GAACTGAAGAGCAAGGAGGAGGAGCAGCAGCGGCTCATCAACGAGCTGACAGCGCAGAGAGCACGCCTGC AGACAGAAGCAGGTGAGTACTCCAGACAGCTGGATGAGAAAGATGCTTTAGTCTCTCAGTTGTCAAGGAG CAAGCAGGCATCCACGCAGCAGATTGAGGAGCTGAAGCGTCAGCTCGAGGAAGAAACCAAGGCCAAGAAC GCGCTGGCCCACGCCCTGCAGTCCTCCCGCCACGACTGTGACCTGCTGCGGGAACAGTATGAAGAGGAGC AGGAAGGCAAAGCCGAGCTGCAGAGGGCACTGTCCAAGGCCAACAGCGAGGTGGCCCAGTGGAGGACCAA ATATGAGACCGATGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAGAAGCTGGCTCAGCGTCTG CAGGCGGCCGAGGAGCACGTAGAAGCCGTGAACGCCAAGTGCGCTTCCCTGGAGAAGACGAAACAGCGGC TGCAGAACGAGGTGGAGGACCTCATGATTGATGTGGAGAGGACCAACGCGGCCTGCGCTGCCCTGGACAA AAAGCAGAGAAACTTCGACAAGGTTCTATCAGAATGGAGACAGAAGTATGAGGAAACTCAGGCTGAGCTT GAGTCCTGCCAGAAGGAGTCCCGAACTCTCAGCACAGAGCTGTTCAAGGTGAAGAACGCCTACGAGGAGT CTCTGGACCACCTGGAGACGCTGAGGAGAGAGAACAAGAATCTGCAACAGGAGATCTCTGACCTGACTGA GCAGATTGCAGAGGGAGGGAAACACATCCATGAGCTGGAGAAAATCAAGAAGCAAGTGGAACAGGAGAAG TGTGAGATACAGGCTGCCTTAGAGGAAGCAGAGGCATCTCTGGAGCACGAGGAGGGAAAGATCCTGCGCA TCCAGCTGGAGCTGAACCAAGTCAAGTCTGAGATCGACAGGAAGATTGCTGAGAAGGATGAGGAGATCGA CCAGCTGAAGAGAAACCACATTAGAGTCGTGGAGTCCATGCAGAGCACTCTGGATGCCGAGATCAGGAGC AGGAATGATGCTCTGAGAGTCAAGAAGAAGATGGAGGGAGACCTGAACGAGATGGAGATCCAGCTGAACC ACGCCAACCGCTTGGCTGCTGAGAGTTTGAGGAACTACAGGAACACACAGGGCATCCTCAAGGACACCCA GCTGCATCTGGATGACGCTCTCCGGGGCCAGGAGGATCTGAAGGAGCAGCTGGCCATCGTGGAGCGCAGA GCCAACCTGCTGCAGGCTGAGATTGAGGAGCTGCGGGCCACGCTGGAGCAGACGGAGAGGAGCAGGAAGA TTGCAGAGCAGGAGCTGCTGGACGCCAGTGAGCGCGTGCAGCTCCTCCACACCCAGAACACGAGCCTCAT CAACACCAAGAAGAAGCTGGAAAATGATGTTTCACAGCTGCAGAGTGAAGTGGAAGAAGTGATTCAAGAG TCACGCAATGCAGAAGAGAAGGCTAAGAAAGCCATTACTGATGCCGCCATGATGGCAGAGGAACTGAAGA AGGAGCAGGACACCAGCGCCCACCTGGAGCGGATGAAGAAGAACATGGAGCAGACCGTGAAGGACCTGCA GCACCGTCTGGACGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATCCAGAAACTGGAGGCC AGGGTACGTGAACTTGAAGGAGAGGTCGAAAATGAACAGAAACGCAATGCTGAGGCTGTTAAAGGGTTAA GGAAGCATGAAAGAAGAGTAAAAGAACTTACCTACCAGACTGAGGAGGACCGCAAGAATGTGCTCCGGCT GCAGGACCTGGTGGACAAATTACAGGCGAAGGTGAAATCCTACAAGAGACAGGCTGAGGAGGCTGAGGAA CAATCCAACGCCAACCTGGCCAAGTTCCGCAAGCTGCAGCATGAGCTGGAGGAAGCCGAGGAGCGGGCTG ACATCGCCGAGTCCCAGGTCAACAAGCTGCGGGTGAAGAGCCGGGAGGTTCACACCAAAATCAGCGCAGA G ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220412 representing NM_177369
Red=Cloning site Green=Tags(s) MSTGSDAEMAIFGEAAPYLRKSEKERIEAQNKPFDAKTSVFVAEPKESYVKSVIQSKDGGKVTVKTESGA TLTVKEDQVFPMNPPKYDKIEDMAMMTHLHEPGVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPE VVAAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGDKKKE ESGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQL KAERSYHIFYQITSNKKPELIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIDILGFSPEEKV SIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQCLNSADLLKALCYPRVKVGNEYVTKGQTV QQVYNAVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQ FFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPLGIFSILEEECMFPKATDTSFKNKLYDQHL GKSNNFQKPKPAKGKAEAHFSLVHYAGTVDYNITGWLDKNKDPLNDTVVGLYQKSAMKTLASLFSTYASA EADGGAKKGAKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNG VLEGIRICRKGFPSRILYGDFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKVFFKA GLLGLLEEMRDEKLAQIITRTQAVCRGYLMRVEYQKMLLRRESIFCIQYNVRAFMNVKHWPWMKLFFKIK PLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKMVTLLKEKNDLQLQVQSEADSLADAEERCEQ LIKNKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKN LTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMD LERAKRKLEGDLKLAQESTMDIENDKQQLDEKLKKKEFEISNLISKIEDEQAVEIQLQKKIKELQARIEE LEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQVEMNKKRETEFQKLRRDLEEATLQHEA TAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSELKMEIDDLSSNAEAIAKAKGNLEKMCRTLEDQVS ELKSKEEEQQRLINELTAQRARLQTEAGEYSRQLDEKDALVSQLSRSKQASTQQIEELKRQLEEETKAKN ALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRL QAAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERTNAACAALDKKQRNFDKVLSEWRQKYEETQAEL ESCQKESRTLSTELFKVKNAYEESLDHLETLRRENKNLQQEISDLTEQIAEGGKHIHELEKIKKQVEQEK CEIQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRVVESMQSTLDAEIRS RNDALRVKKKMEGDLNEMEIQLNHANRLAAESLRNYRNTQGILKDTQLHLDDALRGQEDLKEQLAIVERR ANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLENDVSQLQSEVEEVIQE SRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQHRLDEAEQLALKGGKKQIQKLEA RVRELEGEVENEQKRNAEAVKGLRKHERRVKELTYQTEEDRKNVLRLQDLVDKLQAKVKSYKRQAEEAEE QSNANLAKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKISAE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177369 |
| ORF Size | 5811 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177369.3, NP_796343.2 |
| RefSeq Size | 6149 bp |
| RefSeq ORF | 5814 bp |
| Locus ID | 17885 |
| UniProt ID | P13542 |
| Gene Summary | This gene encodes a myosin heavy chain. The encoded protein forms a hexamer with two heavy chains, two alkali light chains, and two regulatory light chain components. This complex functions in muscle contraction. This gene is located in a cluster of related genes on chromosome 11. [provided by RefSeq, Jun 2013] |
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