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Col4a2 (NM_009932) Mouse Tagged ORF Clone
CAT＃: MG219758
- TrueORF®
Col4a2 (tGFP-tagged) - Mouse collagen type IV alpha 2 (Col4a2), (10ug)
ORF Plasmid: DDK
"NM_009932" in other vectors (2)
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CNY 18,720.00
货期*
9周
规格
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经常一起买 (3)
Mouse monoclonal turboGFP antibody, clone OTI2H8
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Col4; Col4a-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG219758 representing NM_009932
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCGAGTGCGGTTCAAAGCGTCTGGCCCTCCCCTGCGAGGGTGGCTGCTGCTAGCAACTGTGACAG TGGGACTCCTGGCTCAGAGCGTCTTGGGGGGTGTGAAGAAGTTGGATGTGCCCTGCGGAGGGAGAGACTG CAGTGGGGGTTGCCAGTGCTACCCGGAGAAAGGAGCAAGGGGTCAGCCAGGGGCAGTGGGCCCCCAGGGG TACAATGGCCCCCCAGGGTTGCAAGGATTCCCAGGACTACAGGGCCGCAAAGGAGACAAGGGCGAACGGG GAGTTCCTGGACCAACTGGACCAAAAGGAGATGTGGGAGCGAGAGGCGTCTCTGGATTCCCCGGTGCAGA TGGAATTCCTGGGCATCCAGGGCAAGGCGGACCCCGAGGAAGGCCAGGCTACGATGGTTGCAACGGAACC AGGGGAGACGCGGGTCCACAGGGGCCCTCTGGATCTGGGGGCTTCCCTGGCCTCCCTGGGCCCCAAGGAC CCAAGGGGCAGAAAGGCGAGCCTTACGCACTCTCTAAAGAAGACCGTGACAAATACCGGGGTGAACCTGG AGAGCCTGGCCTGGTTGGCTACCAGGGACCTCCTGGCCGCCCAGGGCCCATAGGACAGATGGGTCCCATG GGAGCACCTGGAAGACCGGGACCACCGGGACCCCCTGGACCCAAGGGACAACCAGGCAACCGAGGGCTCG GTTTTTACGGACAGAAGGGTGAAAAGGGTGACATAGGACAGCCAGGACCCAACGGGATCCCATCTGACAT CACACTTGTTGGGCCCACAACATCAACGATCCACCCGGATCTGTACAAGGGTGAAAAAGGAGACGAAGGA GAGCAAGGGATACCCGGCGTAATCTCAAAAGGCGAGGAAGGCATCATGGGGTTCCCAGGAATACGGGGTT TTCCTGGCCTTGATGGAGAAAAAGGAGTCGTAGGACAGAAAGGAAGCAGAGGCCTGGATGGTTTCCAAGG CCCCAGTGGACCTCGAGGACCCAAGGGAGAACGGGGGGAACAAGGACCCCCAGGACCCTCTGTCTACTCG CCCCATCCATCCCTGGCAAAAGGTGCCCGAGGTGACCCAGGATTCCAAGGTGCTCATGGGGAGCCAGGAA GCCGAGGCGAACCGGGAGAGCCTGGAACCGCGGGTCCTCCAGGCCCATCCGTCGGAGATGAAGATTCAAT GAGAGGCCTTCCAGGGGAGATGGGACCCAAAGGCTTCTCAGGAGAACCAGGGTCCCCTGCACGCTATCTT GGCCCACCAGGAGCAGATGGAAGACCAGGTCCCCAAGGAGTCCCTGGACCTGCAGGCCCACCTGGACCAG ATGGCTTCCTGTTTGGCCTGAAAGGATCAGAAGGGAGAGTGGGCTACCCGGGACCTTCAGGTTTCCCAGG AACACGAGGGCAGAAAGGATGGAAAGGTGAAGCTGGAGACTGCCAATGTGGCCAGGTCATCGGGGGTCTT CCGGGACTGCCAGGACCCAAAGGTTTTCCCGGTGTCAATGGGGAGCTCGGAAAGAAAGGCGACCAAGGAG ACCCAGGCCTGCACGGTATCCCTGGGTTCCCAGGATTCAAGGGAGCCCCGGGCGTTGCTGGAGCTCCTGG ACCCAAAGGAATAAAGGGAGACTCTAGAACAATCACCACCAAAGGGGAGCGAGGACAGCCAGGTATCCCT GGTGTACACGGAATGAAAGGTGATGACGGCGTCCCAGGACGGGATGGGCTGGATGGATTCCCTGGCCTGC CTGGCCCCCCGGGCGATGGCATCAAAGGCCCTCCAGGGGATGCAGGTCTCCCTGGTGTACCCGGAACCAA GGGCTTTCCAGGAGACATCGGTCCTCCAGGACAGGGCTTACCAGGTCCGAAAGGCGAGCGTGGTTTCCCT GGAGACGCTGGGTTGCCTGGACCTCCGGGCTTCCCTGGTCCTCCAGGTCCCCCAGGAACCCCAGGACAGA GAGATTGTGACACGGGTGTGAAAAGACCTATCGGAGGCGGCCAGCAAGTGGTCGTTCAGCCAGGTTGCAT TGAAGGCCCCACAGGATCACCTGGCCAGCCAGGACCCCCAGGCCCCACAGGTGCCAAAGGCGTCAGAGGA ATGCCAGGATTCCCAGGAGCTTCTGGAGAACAAGGGCTCAAGGGCTTTCCAGGAGACCCAGGTCGAGAAG GCTTCCCAGGGCCCCCAGGGTTCATGGGACCCCGAGGATCCAAAGGTACCACAGGCCTCCCTGGCCCAGA TGGACCCCCAGGCCCCATTGGACTTCCAGGACCAGCTGGACCCCCTGGGGACAGAGGGATTCCAGGAGAA GTGCTAGGGGCCCAGCCTGGGACACGAGGAGATGCTGGACTTCCAGGACAGCCTGGGCTGAAAGGGCTCC CAGGAGAAACAGGAGCACCGGGATTCAGAGGAAGCCAAGGGATGCCCGGGATGCCAGGGCTTAAAGGTCA GCCAGGCTTCCCAGGACCTTCGGGACAGCCAGGACAGTCTGGGCCCCCAGGACAACATGGATTCCCAGGA ACTCCTGGCCGAGAGGGGCCTTTGGGGCAGCCAGGCTCCCCTGGCCTTGGAGGTTTGCCTGGAGACAGGG GCGAGCCTGGTGACCCTGGTGTCCCTGGCCCTGTGGGCATGAAAGGTCTGTCTGGGGACAGAGGTGACGC CGGTATGTCAGGCGAGCGTGGTCACCCTGGAAGCCCTGGATTTAAAGGAATGGCAGGGATGCCTGGTATC CCTGGGCAGAAAGGTGATCGAGGGTCGCCTGGAATGGACGGTTTCCAGGGCATGTTGGGACTTAAGGGAA GACAGGGCTTCCCAGGGACCAAAGGAGAAGCTGGATTTTTTGGAGTTCCTGGTTTGAAGGGCCTGCCTGG CGAGCCAGGGGTTAAAGGAAACCGAGGAGACCGGGGGCCCCCAGGACCACCTCCTCTCATCCTGCCGGGG ATGAAGGACATCAAGGGAGAAAAGGGAGATGAAGGACCAATGGGCCTGAAAGGGTATCTGGGCTTAAAAG GCATCCAAGGAATGCCCGGAGTCCCCGGAGTGTCTGGATTCCCTGGGCTACCTGGAAGGCCTGGCTTCAT CAAAGGAGTCAAGGGAGACATCGGAGTCCCTGGCACACCAGGCTTGCCGGGATTCCCTGGGGTGTCTGGC CCTCCTGGAATTACCGGGTTTCCAGGATTCACAGGCAGCCGGGGCGAGAAGGGTACTCCAGGAGTAGCAG GAGTTTTTGGCGAGACCGGCCCTACTGGGGACTTTGGTGACATTGGGGACACTGTGGACTTACCAGGGAG CCCAGGCCTGAAGGGGGAACGGGGCATCACGGGAATTCCAGGTCTCAAGGGATTCTTTGGAGAGAAAGGA GCTGCGGGTGACATTGGATTTCCTGGGATAACGGGCATGGCTGGAGCTCAGGGCTCTCCTGGACTTAAAG GACAGACAGGCTTTCCAGGGCTCACAGGGCTGCAAGGGCCACAGGGAGAGCCTGGACGGATCGGAATACC CGGTGACAAGGGTGATTTCGGCTGGCCAGGCGTACCAGGTCTACCAGGTTTTCCTGGGATCCGGGGCATC AGCGGATTGCACGGCCTGCCAGGCACCAAAGGCTTCCCTGGATCACCAGGTGTTGATGCTCATGGAGACC CAGGGTTCCCAGGCCCCACTGGAGACAGGGGTGACCGGGGAGAGGCCAACACACTTCCAGGCCCCGTGGG AGTTCCAGGGCAGAAAGGGGAGCGGGGAACCCCAGGGGAACGTGGCCCAGCTGGAAGCCCAGGACTTCAG GGTTTCCCTGGTATCTCTCCACCATCCAACATCTCTGGGTCACCTGGTGATGTAGGCGCACCAGGAATAT TTGGCTTGCAAGGCTACCAAGGCCCTCCAGGACCACCTGGGCCAAACGCACTTCCTGGAATCAAAGGAGA TGAGGGGAGCTCTGGAGCTGCAGGATTCCCTGGACAGAAAGGATGGGTTGGGGACCCAGGGCCCCAGGGC CAGCCTGGTGTACTCGGTCTTCCGGGGGAGAAAGGGCCCAAGGGTGAACAAGGATTCATGGGCAACACCG GGCCCTCTGGGGCCGTGGGTGACAGAGGCCCCAAAGGACCCAAAGGCGACCAAGGATTCCCAGGTGCTCC TGGCTCTATGGGGTCCCCAGGAATTCCTGGCATCCCCCAGAAGATTGCTGTCCAGCCTGGAACGCTGGGT CCCCAGGGCAGGAGAGGCCTTCCTGGGGCCCTGGGAGAGATAGGGCCGCAGGGCCCTCCTGGAGATCCAG GCTTCCGTGGGGCCCCAGGCAAGGCTGGGCCTCAGGGCAGAGGTGGCGTGTCTGCTGTTCCAGGGTTCCG GGGAGACCAAGGGCCCATGGGACACCAGGGTCCAGTTGGCCAGGAAGGGGAGCCAGGCCGTCCAGGGAGC CCAGGCCTGCCAGGGATGCCAGGCCGCAGTGTGAGCATCGGCTACCTCCTGGTGAAGCACAGCCAAACGG ACCAGGAACCCATGTGCCCTGTGGGCATGAACAAGCTCTGGAGTGGGTACAGCCTGCTATATTTTGAGGG CCAGGAGAAAGCGCACAACCAGGACCTAGGACTGGCAGGCTCCTGCCTGGCACGCTTCAGCACCATGCCT TTCCTGTACTGCAATCCGGGTGACGTCTGCTACTATGCCAGCCGCAACGACAAGTCCTACTGGCTCTCCA CCACGGCCCCTCTGCCCATGATGCCCGTGGCTGAGGAGGAAATCAAGCCCTACATCAGCCGCTGCTCTGT GTGCGAGGCTCCGGCCGTGGCCATTGCCGTGCACAGCCAGGATACCTCTATACCCCACTGCCCGGCTGGG TGGCGGAGTTTGTGGATCGGATATTCATTCCTCATGCACACTGCAGCCGGGGATGAAGGCGGTGGCCAGT CACTGGTGTCGCCGGGCAGCTGTCTAGAGGACTTCCGTGCAACGCCATTTATCGAGTGTAACGGGGGCCG TGGTACCTGCCACTACTTCGCTAACAAGTACAGCTTCTGGCTGACCACGATCCCAGAGCAGAACTTCCAG AGCACACCATCCGCTGACACGCTCAAGGCTGGCCTCATCCGCACGCACATCAGCCGCTGCCAAGTGTGCA TGAAGAATCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219758 representing NM_009932
Red=Cloning site Green=Tags(s) MDRVRFKASGPPLRGWLLLATVTVGLLAQSVLGGVKKLDVPCGGRDCSGGCQCYPEKGARGQPGAVGPQG YNGPPGLQGFPGLQGRKGDKGERGVPGPTGPKGDVGARGVSGFPGADGIPGHPGQGGPRGRPGYDGCNGT RGDAGPQGPSGSGGFPGLPGPQGPKGQKGEPYALSKEDRDKYRGEPGEPGLVGYQGPPGRPGPIGQMGPM GAPGRPGPPGPPGPKGQPGNRGLGFYGQKGEKGDIGQPGPNGIPSDITLVGPTTSTIHPDLYKGEKGDEG EQGIPGVISKGEEGIMGFPGIRGFPGLDGEKGVVGQKGSRGLDGFQGPSGPRGPKGERGEQGPPGPSVYS PHPSLAKGARGDPGFQGAHGEPGSRGEPGEPGTAGPPGPSVGDEDSMRGLPGEMGPKGFSGEPGSPARYL GPPGADGRPGPQGVPGPAGPPGPDGFLFGLKGSEGRVGYPGPSGFPGTRGQKGWKGEAGDCQCGQVIGGL PGLPGPKGFPGVNGELGKKGDQGDPGLHGIPGFPGFKGAPGVAGAPGPKGIKGDSRTITTKGERGQPGIP GVHGMKGDDGVPGRDGLDGFPGLPGPPGDGIKGPPGDAGLPGVPGTKGFPGDIGPPGQGLPGPKGERGFP GDAGLPGPPGFPGPPGPPGTPGQRDCDTGVKRPIGGGQQVVVQPGCIEGPTGSPGQPGPPGPTGAKGVRG MPGFPGASGEQGLKGFPGDPGREGFPGPPGFMGPRGSKGTTGLPGPDGPPGPIGLPGPAGPPGDRGIPGE VLGAQPGTRGDAGLPGQPGLKGLPGETGAPGFRGSQGMPGMPGLKGQPGFPGPSGQPGQSGPPGQHGFPG TPGREGPLGQPGSPGLGGLPGDRGEPGDPGVPGPVGMKGLSGDRGDAGMSGERGHPGSPGFKGMAGMPGI PGQKGDRGSPGMDGFQGMLGLKGRQGFPGTKGEAGFFGVPGLKGLPGEPGVKGNRGDRGPPGPPPLILPG MKDIKGEKGDEGPMGLKGYLGLKGIQGMPGVPGVSGFPGLPGRPGFIKGVKGDIGVPGTPGLPGFPGVSG PPGITGFPGFTGSRGEKGTPGVAGVFGETGPTGDFGDIGDTVDLPGSPGLKGERGITGIPGLKGFFGEKG AAGDIGFPGITGMAGAQGSPGLKGQTGFPGLTGLQGPQGEPGRIGIPGDKGDFGWPGVPGLPGFPGIRGI SGLHGLPGTKGFPGSPGVDAHGDPGFPGPTGDRGDRGEANTLPGPVGVPGQKGERGTPGERGPAGSPGLQ GFPGISPPSNISGSPGDVGAPGIFGLQGYQGPPGPPGPNALPGIKGDEGSSGAAGFPGQKGWVGDPGPQG QPGVLGLPGEKGPKGEQGFMGNTGPSGAVGDRGPKGPKGDQGFPGAPGSMGSPGIPGIPQKIAVQPGTLG PQGRRGLPGALGEIGPQGPPGDPGFRGAPGKAGPQGRGGVSAVPGFRGDQGPMGHQGPVGQEGEPGRPGS PGLPGMPGRSVSIGYLLVKHSQTDQEPMCPVGMNKLWSGYSLLYFEGQEKAHNQDLGLAGSCLARFSTMP FLYCNPGDVCYYASRNDKSYWLSTTAPLPMMPVAEEEIKPYISRCSVCEAPAVAIAVHSQDTSIPHCPAG WRSLWIGYSFLMHTAAGDEGGGQSLVSPGSCLEDFRATPFIECNGGRGTCHYFANKYSFWLTTIPEQNFQ STPSADTLKAGLIRTHISRCQVCMKNL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009932 |
| ORF Size | 5121 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009932.4, NP_034062.3 |
| RefSeq Size | 6426 bp |
| RefSeq ORF | 5124 bp |
| Locus ID | 12827 |
| UniProt ID | P08122 |
| Gene Summary | This gene encodes the alpha-2 subunit of the type IV collagens, an essential component of basement membranes. The encoded protein forms a triple helical heterotrimer comprised of alpha-1 and alpha-2 subunits that assembles into a type IV collagen network. Canstatin, a peptide derived fom the C-terminus of the collagen chain, is a matrikine that has been shown to inhibit angiogenesis. Homozygous knockout mice for this gene exhibit impaired basement membrane integrity and embryonic lethality. This gene shares a bi-directional promoter with a related gene on chromosome 8. [provided by RefSeq, Nov 2015] |
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