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Znrf3 (NM_001080924) Mouse Tagged ORF Clone
CAT＃: MG215754
- TrueORF®
Znrf3 (tGFP-tagged) - Mouse zinc and ring finger 3 (Znrf3), (10ug)
ORF Plasmid: DDK
"NM_001080924" in other vectors (4)
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CNY 8,288.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Gm1167 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG215754 representing NM_001080924
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCCACGCTCGGGCGGGCGCCCAGGGGCCCCGGGCCGCCGCCGCCGCCGCCTCCGCCGCGGCCCCC GCGGTCGTCGCCTGCCGCCGCCGCCGCCGCTGCCGCTGCTTCTCGGGCTGCTGCTGGCGGCCGCGGGGCC CGGCGCGGCGCGGGCCAAGGAGACGGCGTTCGTGGAGGTGGTGCTGTTCGAGTCGAGCCCGAGCGGCGAC TATACCACCCACACCACCGGCCTCACGGGCCGCTTCTCGCGGGCCGGGGCCATGCTCAGCGCCGAGGGCG AGATCGTGCAGATGCACCCACTAGGCCTATGTAATAACAATGACGAAGAGGACTTGTATGAATATGGCTG GGTAGGAGTGGTGAAGCTTGAACAGCCAGAATTGGACCCCAAACCATGTCTCACTGTCCTGGGCAAGGCC AAGAGAGCAGTTCAGCGGGGAGCTACTGCAGTTATCTTTGATGTATCTGAAAATCCAGAAGCCATCGACC AGCTAAACCAAGGCTCAGAAGACCCTCTTAAGAGGCCAGTGGTGTATGTGAAGGGTGCAGATGCTATCAA GCTGATGAACATTGTCAACAAGCAGAAAGTAGCTCGAGCAAGGATCCAGCACCTCCCTCCTCGACAACCC ACCGAGTACTTTGACATGGGAATTTTCCTGGCCTTCTTCGTCGTGGTCTCCTTGGTCTGCCTCATTCTCC TTGTCAAAATCAAGCTGAAGCAACGTCGTAGTCAGAATTCCATGAACAGGCTGGCTGTGCAGGCTCTGGA GAAGATGGAAACCAGAAAGTTCAACTCCAAGAGCAAGGGGCGCCGGGAAGGAAGCTGTGGGGCCCTGGAC ACACTGAGCAGCGGCTCCACATCCGACTGTGCCATCTGTCTGGAGAAGTACATTGACGGAGAGGAGCTTC GGGTCATCCCTTGTACTCATCGGTTCCACAGGAAGTGTGTGGATCCATGGCTACTGCAGCACCACACCTG CCCCCACTGTCGGCACAACATCATAGAACAAAAGGGAAATCCAGGTGCTGTTTGTGTGGAGACAAGCAAC CTTACACGTGGTCGGCAGCCAAGAGTGACCCTACCGGTACATTACCCAGGCCGTGTGCATAGGACCAATG CCATCCCAGCCTACCCTACTAGGACAAGTATGGACTCCCATGGCAACCCTGTCACACTGCTAACCATGGA CCGGCATGGGGAGCAGAACCTCTATTCTCCACAAACACCCACCTATGTCCGTGGCTACCCACCCCTGCAC CTGGACCACACTCTGGCCCCTCATCGCTGCAGCCTGGAACACCGGGCCTACTCACCAGCCCATCCCTTCC GCAGGCCCAAGTTCAGCAGCCGCAGCTTCTCCAAGGCAGCTTGCTTCTCCCAGTATGAGACCATGTACCA ACACTACTACTTCCAGGGTCTCAGCTACCCAGAGCAGGAGGGCCAGACAATACCCAGCGTCACACCCAGG GGCCAATCCCGTGCCTTCCCACCAAGTGGTGCCAGCAGCCTGCTCTTCCCCACAATGGTACATGTGGCCC CTCCAACCCACGTGGAGAGTGGCAGCACATCTAGCTTCAGTTGCTATCATGGGCACCGTTCTGTGTGTAG CGGCTACCTGGCTGACTGTCCTGGCAGCGATAGCAGCAGCAACAGCTCTGGCCAGTGCCGCTGCTCTTCC AGTGACTCCGTGGTGGACTGCACAGAAGTCAGCAACCAAGGTGTGTATGGGAGCTGTTCCACCTTCCGAA GCTCCCTCAGCAGCGACTATGACCCCTTCATCTACCGCAGCCGGGGGCCTGCTGTGCACCTTGAGGGCTC CCCTCCACCAGAGGAGCTCCCAGCAGGACACAGTCAAAGTGCTGGGAGGGGCGAGCCATGGCTAGGCCCT GCCTCTCCCTCAGGGGACCAGCTGTCCACCTGCAGCCTGGAGATGAACTACAGCAGCAACTCCTCGCTGG AACCCAGAGGGCCCAACAGCTCTACCTCAGAAGTGGGGCTCGAGGTTTCTCCTGGGGCTGCCCTGGACCT CAGGAGGACCTGGAAAGGGGGTCCAGAGGGACCATCATGTGCTTGCTGCTTCGAGCCCCAGCCCTTCCCG CCGGGCTCTGGGATAGAAACAAGTGCAGGTGGCAGCAGTTTATTCCTGGGTCCTCGGCTCCTCGAGGACT GCAACCCTCCAAGTGGGGAGCCACAGCTGGGGAGCTCCCAGGGCCTGTATGGTCTTCACTCAGACCATTA TCCTAGGACAGATGGGGTGAAATATGAGGGCTTGCCCTGCTGCTTCTATGAAGAGAAGCAGGTGGCCCAC AGTGCTGGCAGGGGCAATGGCTGCTACACTGAGGACTATTCAGTGAGTGTACAGTACACACTCACCGAGG AACCCCCACCCAGCTGTTATGCAGGGCCCCGGGACCTGAGCCAGCGCATCCCCATTATTCCAGAGGATGT AGACTGTGACTTGGGCTTGCCCCAAGACTGCCACGGGATGCACAACCACAGCCCCTGGGGTGGGGCACTG AGCTTGGACGTTCCCCGACTCCACTGGAGTCTAGGCACAACCCGGGAAGAGGAACAGGCTCCATGTTACC AAGCTGAGGTGCAGCCTGGCTGCTCTCCAGAGGAGGCAGGTGCTTCCAGGGCTAGCCTCTCCAGTGCCCC CCAGGACACTCAGGAGTCTCATGCCCTGGCTGCTGAGGCTTCAGGACCAGGATCTGGCCCAGGCATCGGT ACGGGAGCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215754 representing NM_001080924
Red=Cloning site Green=Tags(s) MRPRSGGRPGAPGRRRRRLRRGPRGRRLPPPPPLPLLLGLLLAAAGPGAARAKETAFVEVVLFESSPSGD YTTHTTGLTGRFSRAGAMLSAEGEIVQMHPLGLCNNNDEEDLYEYGWVGVVKLEQPELDPKPCLTVLGKA KRAVQRGATAVIFDVSENPEAIDQLNQGSEDPLKRPVVYVKGADAIKLMNIVNKQKVARARIQHLPPRQP TEYFDMGIFLAFFVVVSLVCLILLVKIKLKQRRSQNSMNRLAVQALEKMETRKFNSKSKGRREGSCGALD TLSSGSTSDCAICLEKYIDGEELRVIPCTHRFHRKCVDPWLLQHHTCPHCRHNIIEQKGNPGAVCVETSN LTRGRQPRVTLPVHYPGRVHRTNAIPAYPTRTSMDSHGNPVTLLTMDRHGEQNLYSPQTPTYVRGYPPLH LDHTLAPHRCSLEHRAYSPAHPFRRPKFSSRSFSKAACFSQYETMYQHYYFQGLSYPEQEGQTIPSVTPR GQSRAFPPSGASSLLFPTMVHVAPPTHVESGSTSSFSCYHGHRSVCSGYLADCPGSDSSSNSSGQCRCSS SDSVVDCTEVSNQGVYGSCSTFRSSLSSDYDPFIYRSRGPAVHLEGSPPPEELPAGHSQSAGRGEPWLGP ASPSGDQLSTCSLEMNYSSNSSLEPRGPNSSTSEVGLEVSPGAALDLRRTWKGGPEGPSCACCFEPQPFP PGSGIETSAGGSSLFLGPRLLEDCNPPSGEPQLGSSQGLYGLHSDHYPRTDGVKYEGLPCCFYEEKQVAH SAGRGNGCYTEDYSVSVQYTLTEEPPPSCYAGPRDLSQRIPIIPEDVDCDLGLPQDCHGMHNHSPWGGAL SLDVPRLHWSLGTTREEEQAPCYQAEVQPGCSPEEAGASRASLSSAPQDTQESHALAAEASGPGSGPGIG TGA TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001080924 |
| ORF Size | 2739 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001080924.2, NP_001074393.1 |
| RefSeq Size | 6306 bp |
| RefSeq ORF | 2742 bp |
| Locus ID | 407821 |
| UniProt ID | Q5SSZ7 |
| Gene Summary | E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone (PubMed:22575959, PubMed:22895187). Along with RSPO2 and RNF43, constitutes a master switch that governs limb specification (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222618 | Znrf3 (untagged) - Mouse zinc and ring finger 3 (Znrf3), (10ug) |
CNY 11,780.00 |
|
| MR215754 | Znrf3 (Myc-DDK-tagged) - Mouse zinc and ring finger 3 (Znrf3) |
CNY 6,688.00 |
|
| MR215754L3 | Lenti ORF clone of Znrf3 (Myc-DDK-tagged) - Mouse zinc and ring finger 3 (Znrf3) |
CNY 9,030.00 |
|
| MR215754L4 | Lenti ORF clone of Znrf3 (mGFP-tagged) - Mouse zinc and ring finger 3 (Znrf3) |
CNY 9,030.00 |
