Hr (BC049182) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	N, AU, ba, rh, ALUNC, rhino
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211766 representing BC049182 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGTATGCCCAGCTTCCTGAAGGACACCCCAGCCTGGGAGAAGACAGCCCCTGTGAACGGCATTG TGGGACAGGAGCCTGGTACCTCACCACAGGATGGCTTGCGCCATGGGGCACTGTGCCTAGGAGAACCTGC TCCCTTTTGGAGGGGTGTTCTGAGCACCCCAGACTCCTGGCTCCCTCCTGGCTTCCTCCAAGGCCCCAAG GACACACTCTCACTGGTGGAGGGTGAGGGTCCTCGGAACGGGGAGAGGAAGGGCAGCTGGCTAGGCGGCA AGGAAGGACTGCGCTGGAAGGAAGCGATGCTGGCCCATCCACTGGCCTTTTGTGGGCCGGCATGCCCACC TCGCTATGGGCCCCTGATACCTGAGCATAGTGGTGGCCATCCCAAGAGTGACCCTGTGGCCTTCCGGCCC TTGCACTGCCCTTTCCTGCTGGAGACCAAGATTCTAGAGCGAGCTCCCTTCTGGGTTCCTACCTGCTTGC CTCCCTACCTGATGTCCAGCCTGCCCCCAGAGCGTCCATATGACTGGCCTTTGGCCCCAAACCCATGGGT ATACTCTGGGAGCCAGCCAAAAGTGCCCTCTGCCTTTGGCTTAGGCAGCAAGGGCTTTTACCACAAGGAT CCGAACATCCTCAGGCCAGCAAAGGAGCCCTTGGCAGAGTCTGGGATGTTGGGCTTAGCCCCTGGTGGGC ATCTCCAGCAAGCCTGTGAATCAGAAGGCCCCTCACTTCACCAGAGGGATGGGGAGACAGGAGCTGGCAG GCAGCAGAATCTTTGCCCAGTTTTCTTGGGGTACCCAGACACTGTTCCTCGGGCCCCCTGGCCTTCTTGT CCCCCAGGCCTGGTTCACAGTCTTGGCAACATTTGGGCTGGGCCAGGAAGTAATAGCCTTGGGTACCAAC TAGGACCACCAGCCACACCAAGGTGCCCATCTCCTGGGCCTCCTACCCCTCCAGGGGGCTGTTGCTCATC CCATCTACCTGCCAGAGAGGGGGATCTTGGCCCTTGTAGGAAATGCCAGGATAGCCCAGAGGGAGGTAGC AGTGGGCCAGGGGAATCCAGTGAAGAAAGGAACAAAGCTGATTCCAGGGCCTGTCCCCCAAGCCATCACA CCAAGCTGAAAAAGACCTGGCTCACACGCCACTCGGAACAGTTTGAGTGCCCAGGTGGCTGTTCAGGGAA GGAAGAGAGCCCAGCCACTGGGCTCCGGGCACTCAAGAGGGCAGGCAGTCCAGAGGTCCAAGGAGCATCA AGGGGCCCAGCTCCCAAACGCCCATCCCACCCTTTCCCAGGCACTGGGAGGCAGGGGGCCAGGGCTTGGC AGGAGACACCGGAGACAATCATAGGAAGCAAGGCGGAGGCAGAGCAACAAGAGGAACAGAGAGGGCCCCG AGATGGCAGGATTAGGCTCCAGGAGTCCAGACTTGTGGATACATCCTGCCAGCATCACTTAGCAGGTGTC ACCCAGTGCCAAAGCTGTGTCCAGGCAGCTGGAGAGGTAGGGGTACTGACCGGCCACTCCCAGAAATCAC GTAGGTCACCCCTGGAGGAGAAGCAGTTGGAGGAGGAGGATTCCTCTGCCACTTCCGAAGAAGGAGGAGG AGGGCCTGGCCCAGAAGCTTCACTCAACAAGGGCCTGGCCAAGCACCTGCTGAGTGGTTTGGGGGACCGA CTCTGCCGCCTGCTGCGGAAGGAGCGGGAGGCCCTTGCCTGGGCACAGCGAGAAGGCCAGGGGCCAGCCA TGACAGAGGACAGCCCAGGCATTCCACATTGCTGCAGTCGATGCCACCACGGACTCTTCAACACCCACTG GAGATGTTCCCACTGTAGCCACCGGCTGTGTGTAGCCTGTGGTCGCATAGCCGGTGCTGGAAAGAACAGG GAGAAAACAGGTTCTCAGGAACAGCACACAGATGACTGCGCCCAGGAGGCTGGGCATGCTGCCTGTTCCC TGATCCTGACCCAGTTTGTCTCCAGCCAGGCGCTGGCAGAACTGAGCACTGTGATGCACCAAGTCTGGGC CAAGTTTGACATTCGGGGGCACTGTTTCTGCCAGGTTGATGCCCGTGTGTGGGCCCCCGGGGATGGGGGT CAGCAGAAGGAACCAACAGAGAAAACTCCCCCAACTCCACAACCTTCCTGCAATGGAGATTCCAATCGGA CCAAGGACATCAAAGAAGAGACCCCAGACTCCACTGAGAGCCCAGCAGAGGATGGTGCTGGCCGGTCACC CCTTCCTTGTCCCTCTCTCTGTGAGCTGCTAGCCTCTACTGCTGTCAAACTCTGCCTGGGGCATGACCGG ATTCACATGGCCTTTGCTCCGGTCACCCCAGCTCTGCCCAGTGATGACCGCATTACCAACATCCTGGACA GCATTATTGCGCAGGTAGTAGAACGGAAGATCCAAGAGAAAGCCCTGGGGCCAGGCCTGCGAGCAGGGTC AGGCTTACGCAAGGGCCTGAGCCTTCCATTGTCACCAGTGCGAACCCGGCTGTCTCCTCCTGGAGCTTTG CTGTGGCTGCAGGAGCCCAGGCCTAAGCATGGCTTCCATCTCTTCCAGGAACACTGGCGGCAGGGCCAGC CCGTGTTAGTGTCAGGCATCCAGAAGACATTGAGACTTAGCCTGTGGGGAATGGAAGCCCTTGGGACACT TGGTGGCCAGGTGCAGACACTGACTGCCCTTGGGCCTCCCCAGCCCACAAACCTGGACAGCACAGCATTC TGGGAGGGATTCTCTCATCCTGAGACACGTCCAAAGTTAGATGAGGGCTCTGTCCTCCTGCTACACCGAA CCCTGGGGGATAAGGACGCTAGCAGGGTGCAGAACCTTGCCTCCAGCCTTCCACTCCCAGAATACTGTGC CCACCAAGGGAAACTCAACCTAGCGTCCTACCTCCCCCTGGGCCTCACACTGCATCCACTGGAGCCCCAG CTCTGGGCGGCCTATGGTGTGAACTCACACCGTGGACACCTGGGGACCAAGAATCTATGCGTGGAGGTGT CTGACCTAATCAGTATCCTGGTGCACGCCGAGGCCCAGCTGCCTCCCTGGTATCGAGCACAGAAAGATTT CCTCTCAGGCCTGGATGGGGAAGGACTCTGGTCTCCAGGGAGCCAGACCAGCACTGTGTGGCATGTGTTC CGGGCCCAGGATGCCCAGCGCATCCGTCGCTTTCTCCAGATGGTGTGCCCAGCTGGAGCAGGAACCTTGG AGCCTGGTGCCCCAGGCAGCTGCTACTTGGATGCAGGGTTGCGCCGACGGCTAAGAGAAGAGTGGGGTGT GAGCTGCTGGACCCTGCTGCAGGCTCCTGGGGAAGCGGTGCTGGTCCCGGCTGGGGCGCCCCATCAGGTG CAGGGCCTGGTGAGCACAATCAGTGTCACTCAGCACTTTCTGTCTCCTGAGACCTCTGCCCTCTCTGCTC AGCTCTACCACCAGGGAGCCAGCCTACCCCCTGACCACCGTATGCTTTATGCCCAGATGGACCGGGCTGT GTTCCAAGCAGTAAAGGCGGCTGTGGGGGCGTTACAGGAAGCTAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211766 representing BC049182 Red=Cloning site Green=Tags(s) MESMPSFLKDTPAWEKTAPVNGIVGQEPGTSPQDGLRHGALCLGEPAPFWRGVLSTPDSWLPPGFLQGPK DTLSLVEGEGPRNGERKGSWLGGKEGLRWKEAMLAHPLAFCGPACPPRYGPLIPEHSGGHPKSDPVAFRP LHCPFLLETKILERAPFWVPTCLPPYLMSSLPPERPYDWPLAPNPWVYSGSQPKVPSAFGLGSKGFYHKD PNILRPAKEPLAESGMLGLAPGGHLQQACESEGPSLHQRDGETGAGRQQNLCPVFLGYPDTVPRAPWPSC PPGLVHSLGNIWAGPGSNSLGYQLGPPATPRCPSPGPPTPPGGCCSSHLPAREGDLGPCRKCQDSPEGGS SGPGESSEERNKADSRACPPSHHTKLKKTWLTRHSEQFECPGGCSGKEESPATGLRALKRAGSPEVQGAS RGPAPKRPSHPFPGTGRQGARAWQETPETIIGSKAEAEQQEEQRGPRDGRIRLQESRLVDTSCQHHLAGV TQCQSCVQAAGEVGVLTGHSQKSRRSPLEEKQLEEEDSSATSEEGGGGPGPEASLNKGLAKHLLSGLGDR LCRLLRKEREALAWAQREGQGPAMTEDSPGIPHCCSRCHHGLFNTHWRCSHCSHRLCVACGRIAGAGKNR EKTGSQEQHTDDCAQEAGHAACSLILTQFVSSQALAELSTVMHQVWAKFDIRGHCFCQVDARVWAPGDGG QQKEPTEKTPPTPQPSCNGDSNRTKDIKEETPDSTESPAEDGAGRSPLPCPSLCELLASTAVKLCLGHDR IHMAFAPVTPALPSDDRITNILDSIIAQVVERKIQEKALGPGLRAGSGLRKGLSLPLSPVRTRLSPPGAL LWLQEPRPKHGFHLFQEHWRQGQPVLVSGIQKTLRLSLWGMEALGTLGGQVQTLTALGPPQPTNLDSTAF WEGFSHPETRPKLDEGSVLLLHRTLGDKDASRVQNLASSLPLPEYCAHQGKLNLASYLPLGLTLHPLEPQ LWAAYGVNSHRGHLGTKNLCVEVSDLISILVHAEAQLPPWYRAQKDFLSGLDGEGLWSPGSQTSTVWHVF RAQDAQRIRRFLQMVCPAGAGTLEPGAPGSCYLDAGLRRRLREEWGVSCWTLLQAPGEAVLVPAGAPHQV QGLVSTISVTQHFLSPETSALSAQLYHQGASLPPDHRMLYAQMDRAVFQAVKAAVGALQEAK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC049182
ORF Size	3548 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC049182, AAH49182
RefSeq Size	5599 bp
RefSeq ORF	3548 bp
Locus ID	15460
Gene Summary	This gene encodes a protein that is involved in hair growth. This protein functions as a transcriptional corepressor of multiple nuclear receptors, including thyroid hormone receptor, the retinoic acid receptor-related orphan receptors and the vitamin D receptors, and it interacts with histone deacetylases. The translation of this protein is modulated by a regulatory ORF that exists upstream of the primary ORF. Mutations in this upstream ORF, U2HR, cause Marie Unna hereditary hypotrichosis (MUHH), an autosomal dominant form of genetic hair loss in human. [provided by RefSeq, Oct 2014]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.